標簽內參抗體
標簽抗體
標簽抗體，是指能夠高度特異性識別通過重組技術構建的融合蛋白上特殊表位的抗體，可用于檢測和純化多種具有相同標記的蛋白質。抗原表位，又稱抗原決定簇，是抗原分子中決定抗原特異性的特殊區域或基團，是與抗體特異性結合的結構或序列。隨著生物技術的發展，科研人員可以通過DNA重組技術，構建包含目的基因以及表位標記的融合蛋白，進而通過特異性標簽抗體對其鑒定與純化，以達到研究的需求。
華美生物提供6*His、GFP、c-MYC、GST、E-Tag、HA-Tag、Flag Tag、Sumo Tag等標簽抗體，通過抗原親和純化，可與相應的標記蛋白特異性結合，從而鑒定和純化目標蛋白。種類齊全、特異性高，可保證您實驗的順利進行。
| Target | Applications | Target | Applications |
|---|---|---|---|
| 6*His | ELISA, WB | GFP | ELISA,WB,IP |
| c-MYC | ELISA, WB, IF, IP | GST | ELISA,WB |
| E-Tag | ELISA, WB, IP | HA-Tag | ELISA, WB, IF, FC, IP |
| Flag Tag | ELISA, WB, IP | Sumo tag | ELISA,WB |
內參抗體
內參即是內部參照（Internal Control），對于哺乳動物細胞表達來說一般是指由管家基因編碼表達的蛋白(Housekeeping Proteins)，它們在各組織和細胞中的表達相對恒定，在檢測蛋白的表達水平變化時常用它來做參照物。
華美生物提供的內參抗體包括Beta-actin、GAPDH、PCNA、TUBAIA等，性能穩定，質量可靠，可長期穩定儲存，可在進行Western blot實驗時檢測目的產物、校正上樣誤差，也可作為空白對照，檢測蛋白轉膜是否完全、整個顯色或者發光體系是否正常。
| Target | Applications | Target | Applications |
|---|---|---|---|
| GAPDH | ELISA, WB, IHC, IP, IF, FC | TUBA1A | ELISA, WB, IHC, IF, FC, IP |
| Beta-Actin | ELISA | PCNA | ELISA |
產品優勢
- 種類齊全，特異性高
- 高靈敏度，可檢測較低表達水平蛋白
Western Blot analysis of HA-Tag Monoclonal Antibody (CSB-MA000141M0m)
Western Blot analysis of E-Tag Monoclonal Antibody (CSB-MA000151M0m)
Western Blot analysis of GAPDH Monoclonal Antibody (CSB-MA000071M0m)
- 高穩定性，批內差/批間差較小
- 經過WB、FC、IP、IF、IHC等多重驗證
HA-Tag Monoclonal Antibody (CSB-MA000141M0m)
Western Blot (WB)
Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA-Tag antibody at 1:1000
Flow cytometry (FC)
Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100.
Immunoprecipitation (IP)
Immunoprecipitating HA-Tag in 293F transfected whole cell lysate.
Lane 1: Mouse control IgG (1μg); Lane 2: CSB-MA000141M0m (5μg) + 293F transfected whole cell lysate (500μg); Lane 3: 293F transfected whole cell lysate (20μg)
Immunofluorescence (IF)
Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI.
GAPDH Monoclonal Antibody (CSB-MA000071M0m)
Western Blot (WB)
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000
Immunoprecipitation (IP)
Immunoprecipitating GAPDH in Hela whole cell lysate. Lane 1: Mouse control IgG; Lane 2: CSB-MA000071M0m (3μl) + Hela whole cell lysate (500μg); Lane 3: Hela whole cell lysate (20μg)
Flow cytometry (FC)
Overlay histogram showing Hela cells stained with CSB-MA000141M0m (red line) at 1:200.
Immunofluorescence (IF)
Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI.
Immunohistochemistry (IHC)
IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.
產品目錄
| 產品名稱 | 貨號 | 規格 |
|---|---|---|
| Avi-Tag Monoclonal Antibody | CSB-MA000101M0m | 100μl/50μl |
| ACTB Monoclonal Antibody | CSB-MA000091M2m | 100μl/50μl |
| Flag-Tag Monoclonal Antibody | CSB-MA249923 | 100μl/50μl |
| His Monoclonal Antibody | CSB-MA000011M1m | 100μl/50μl |
| His Monoclonal Antibody, Biotin conjugated | CSB-MA000011D0m | 100μl/50μl |
引用文獻
Myc tag Monoclonal Antibody referenced in "Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex". The Journal of Cell Biology, 2019
His-Tag Monoclonal Antibody referenced in "When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell". Toxicon, 2019
Flag Tag Monoclonal Antibody referenced in "The role of the cytoplasmic tail of the influenza virus M2 protein in viral morphology and replication". University of Kent, 2018
His-Tag Monoclonal Antibody referenced in "Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus". Nature Plants, 2018
