GAPDH Monoclonal Antibody

文獻引用 (5) 說明書

中文名稱：

GAPDH鼠單克隆抗體
貨號：

CSB-MA000071M0m
規格：

￥400
圖片：
Western Blot
Positive WB detected in: 15μg hela whole cell lysate
GAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: Hela whole cell lysate at 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
All lanes: GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 30s

Western Blot
Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 30s

Western Blot
Positive WB detected in: Rat heart tissue, Rat kidney tissue, Rat skeletal muscle tissue, Rat liver tissue, Rat brain tissue tissue, Rat spleen tissue
All lanes GAPDH antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 1min

Western Blot
Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min

IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of HepG2 cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunoprecipitating GAPDH in Hela whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA000071M0m in Hela whole cell lysate.
Lane 2: CSB-MA000071M0m (1μl) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
For western blotting, the blot was detected with CSB-MA000071M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

Overlay histogram showing Hela cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Overlay histogram showing Jurkat cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

1.Exosomes extracted from HEPG2 cells
2. Exosomes extracted from PC-3 cells
3. Exosomes extracted from Hela cells
4. Exosomes extracted from U87 cells
5. Hela cell Lysate

1.Exosomes extracted from MG63 cells
2. Exosomes extracted from Ntera-2 cells
3. MG63 cell Lysate

1. Exosomes extracted from Raji cells
2. Exosomes extracted from U251 cells
3. Raji cell Lysate
其他：

產品詳情
產品評價
引用文獻
相關產品
問答及客戶評論
靶點詳情

產品詳情

產品描述：

CUSABIO貨號：CSB-MA000071M0m號GAPDH單克隆抗體是針對糖酵解關鍵酶甘油醛-3-磷酸脫氫酶（GAPDH）研發的高特異性科研工具，適用于人、大鼠、兔等多物種檢測。該抗體經嚴格驗證可支持ELISA、Western Blot、免疫組化、免疫沉淀、免疫熒光及流式細胞術六大實驗平臺，其36kDa特征性檢測條帶能為蛋白質定量提供可靠參照。作為廣泛認可的內參指標，GAPDH抗體在基因表達分析、代謝通路研究及疾病機制探索中發揮重要作用，特別適用于腫瘤生物學、神經科學等領域的基礎研究。產品采用高純度抗原免疫制備，通過蛋白A親和純化工藝確保批間一致性，為科研工作者提供穩定可靠的實驗數據支持。
產品名稱：

Mouse anti-Homo sapiens (Human) GAPDH Monoclonal antibody
Uniprot No.：

P04406
基因名：

GAPDH
別名：

GAPDH; G3PD; GAPD; MGC88685
宿主：

Mouse
反應種屬：

Human, Rat, Rabbit
免疫原：

Recombinant Human GAPDH protein (3-335AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG1
純化方式：

>95%, Protein G purified
克隆號：

14C2F11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IP, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:5000-1:1600000
IHC	1:50-1:500
IF	1:50-1:200
IP	1µl-2µl
FC	1:100-1:300

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

Senescence of endothelial cells increases susceptibility to Kaposi's sarcoma-associated herpesvirus infection via CD109-mediated viral entry MJ Lee, JH Yeon, J Lee, YH Kang, BS Park,Journal of Clinical Investigation,2024
Salidroside ameliorates diabetic retinopathy and Müller cell inflammation via the PI3K/Akt/GSK-3β/NF-?B pathway Z Feng,/,2024
CXCL1 confers a survival advantage in Kaposi's sarcoma‐associated herpesvirus‐infected human endothelial cells through STAT3 phosphorylation MJ Lee,Journal of medical virology,2023
Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane B Lee.et al,Biochimica et biophysica acta-molecular cell research,2017
A fluorescence method for determination of glucose transport by intestinal BBMV of common carp Yang LP.et al,Anal Biochem,2017

產品評價

問答及客戶評論

■ 客戶評論

Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: Culture cell (L-02)
Sample volume: 15μg
Primary antibody dilution ratio: 1:50,000
Review: 1:5w requires a long exposure time and is noisy. It is expected that the effect of using 1:5k should be better. Overall, the WB effect is satisfactory. It is hoped that other label antibodies with high dilution ratio can be offered for trial.
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: Human ovarian granulosa cell carcinoma
Sample volume: 12μg, 10μg, 8μg
Primary antibody dilution ratio: 1:1200
Review: Experiment success, antibody is usable!
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: KELLY
Sample volume: 30μg
Primary antibody dilution ratio: 1:1000
Review: GAPDH has good results in this experiment, with correct size and single band.
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: 293T Cell
Sample volume: 20μL
Primary antibody dilution ratio: 1:2000
Review: The protein is normal with clear bands, meeting the requirements of the experiment, with correct size and single bands.
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: Human ovarian granulosa cell carcinoma
Sample volume: 10μg
Primary antibody dilution ratio: 1:1000
Review: The experiment was successful, and the antibody was successfully detected on human cells, which meets the experimental requirements and can be purchased and used in the future.
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: Rat kidney tissue
Sample volume: 50μg
Primary antibody dilution ratio: 1:5000
Review: Good antibody, good titer.
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: Mouse
Primary antibody dilution ratio: 1:5000
Review: Antibody specificity is very good.
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: Human 231 cell
Primary antibody dilution ratio: 1:2000
Review: The destination band is correct and single.
Western blot review for CD146 Monoclonal Antibody

Applications: WB
Review: Immunoblot analyses show the presence of SGLT-1 on BBMV of common carp. Left lane: SGLT-1: BBMV. Right lane: GAPDH.
PMID: 28847591
Western blot review for CD146 Monoclonal Antibody

Applications: WB
Review: HeLa cells were treated with si BAF #2 or si Control for 72 h. Cells were fractionated and subjected to western blot analyses (upper panel). Fractionation was verified by using Lamin A/C antibody (nuclear fraction) or GAPDH antibody (cytosolic fraction). The intensity of the cytosolic emerin was analyzed using ImageJ software (lower panel).
PMID: 27865926
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample&species: Cell Lysate (Chicken)
Primary antibody dilution ratio: 1:2000
Review: Incubate at 4 °C overnight with 1: 2000 dilution ratio, the bands are clear, the specificity is good, and it can be recycled and reused with good results.

靶點詳情

功能：

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
基因功能參考文獻：
1. This suggests that RX624 might be useful as a drug against polyglutamine pathologies, and that is could be administered exogenously without affecting target cell physiology. This protective effect was validated by the similar effect of an anti-GAPDH specific antibody. PMID: 28450110
2. GAPDH can interact with proteins participating in DNA repair, such as APE1, PARP1, HMGB1, and HMGB2. In this review, the functions of GAPDH associated with DNA repair are discussed in detail. PMID: 28601074
3. Nitric oxide-induced GAPDH aggregation specifically induces mitochondrial dysfunction via permeability transition pore opening, leading to cell death. PMID: 28167533
4. GAPDH may act as a chaperone in heme transfer to downstream areas PMID: 28315300
5. NAD(+) inhibited both GAPDH aggregation and co-aggregation with GOSPEL, a hitherto undescribed effect of the coenzyme against the consequences of oxidative stress. PMID: 27282776
6. Monoclonal Antibodies DSHB-hGAPDH-2G7 and DSHB-hGAPDH-4B7 Against Human Glyceraldehyde-3-Phosphate Dehydrogenase. PMID: 27556912
7. the present study suggests that GAPDH plays an important role in cancer metastasis by affecting EMT through regulation of Sp1-mediated SNAIL expression. PMID: 27878251
8. Knockdown of LAMP2A, a CMA-related protein, and TSG101, an mA-related protein, significantly but only partially decreased the punctate accumulation of GAPDH-HT in AD293 cells and primary cultured rat cortical neurons. PMID: 27377049
9. In conclusion, the data show that two GAPDH binders could be therapeutically relevant in the treatment of injuries stemming from hard oxidative stress. PMID: 26748070
10. transient silencing of GAPDH reduces intracellular ROS and facilitates increased autophagy, thereby reducing acute hypoxia and reoxygenation injury as well as the resulting apoptosis and necrosis. PMID: 26279122
11. This review will summarize our current understanding of GAPDH-mediated regulation of RNA function PMID: 26564736
12. analysis of PSCA level in the peripheral blood of PC patients who underwent radical prostatectomy shows it is related to a GADPH reference level (PSCA/GAPDH ratio) PMID: 26527100
13. In 60 % of patients with type 2 diabetes, a reversible inhibition of GAPDH is observed. PMID: 25189828
14. The results of this study led us to conclude that in cancer cells constantly exposed to conditions of oxidative stress, the protective power of Hsp70 should be abolished by specific inhibitors of Hsp70 expression. PMID: 26713364
15. GAPDH and protoporphyrinogen oxidase were shown to have higher expression in faster growing cell lines and primary tumors. Pharmacologic inhibition of GAPDH or PPOX reduced the growth of colon cancer cells in vitro PMID: 25944804
16. The levels of GAPDH protein were significantly up-regulated in lung squamous cell carcinoma tissues and elevated GAPDH expression is associated with the proliferation and invasion of lung and esophageal squamous cell carcinomas. PMID: 25944651
17. Genetic variants in GAPDH confer susceptibility to sporadic Parkinson's disease in a Chinese Han population. PMID: 26258539
18. Data revealed that GAPDH is a phosphorylation substrate for AMPK and its interaction with Sirt1 in the nucleus. The phosphorylation and the nuclear translocation of GAPDH mediate rapid Sirt1 activation and autophagy initiation under glucose deprivation. PMID: 26626483
19. findings demonstrate that dissociation of the GAPDH/Siah1 pro-apoptotic complex can block high glucose-induced pericyte apoptosis, widely considered a hallmark feature of diabetic retinopathy PMID: 26438826
20. Extracellular GAPDH, or its N-terminal domain, inhibited gastric cancer cell growth. GAPDH bound to E-cadherin and downregulated the mTOR-p70S6 kinase pathway. PMID: 25785838
21. suggests that GAPDH aggregates accelerate Abeta amyloidogenesis, subsequently leading to mitochondrial dysfunction and neuronal cell death in the pathogenesis of AD PMID: 26359500
22. The level of GAPDH-AP DNA adduct formation depends on oxidation of the protein SH-groups; disulfide bond reduction in GAPDH leads to the loss of its ability to form the adducts with AP DNA PMID: 26203648
23. The activity of GAPDS was significantly positively correlated with sperm motility and negatively with the incidence of infertility. PMID: 26255202
24. The N terminus of nuclear GAPDH binds with PARP-1, and this complex promotes PARP-1 overactivation both in vitro and in vivo. PMID: 25882840
25. deregulated GAPDH expression promotes NF-kappaB-dependent induction of HIF-1alpha and has a key role in lymphoma vascularization and aggressiveness PMID: 25394713
26. analysis of how flux through GAPDH is a limiting step in aerobic glycolysis PMID: 25009227
27. astrocytic production of D-serine is modulated by glycolytic activity via interactions between GAPDH and SRR. PMID: 25870284
28. Dimer and tetramer interface residues in adenine-uridine rich elements are important for GAPDH-RNA binding. PMID: 25451934
29. Siah1 is a substrate of ASK1 for activation of the GAPDH-Siah1 oxidative stress signaling cascade. PMID: 25391652
30. GAPDH expression is deregulated during melanoma progression. PMID: 25550585
31. Oxidation of an exposed methionine instigates the aggregation of glyceraldehyde-3-phosphate dehydrogenase. PMID: 25086035
32. MZF-1 binds to and positively regulates the GAPDH promoter, indicating a role for GAPDH in calcitriol-mediated signaling. PMID: 25065746
33. The protein encoded by this gene contains a peptide that displays antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. PMID: 22832495
34. GAPDH gene over expression in resected tumor samples is an adverse prognostic factor in non small cell lung cancer. PMID: 23988223
35. This review describes the structure and localization of GAPDH in cells as well as the latest discoveries on the multifunctional properties of the enzyme. PMID: 24018444
36. TG2-dependent GAPDH deamidation was suggested to participate in actin cytoskeletal remodeling. PMID: 24375405
37. acetylation of GAPDH (K254) is reversibly regulated by the acetyltransferase PCAF and the deacetylase HDAC5. PMID: 24362262
38. GAPDH binds to active Akt, leading to Bcl-xL increase and escape from caspase-independent cell death. PMID: 23645209
39. GAPDH is a moonlighting protein that functions as a glycolytic enzyme as well as a uracil DNA glycosylase. PMID: 20727968
40. Results indicate that CIB1 is uniquely positioned to regulate PI3K/AKT and MEK/ERK signaling and that simultaneous disruption of these pathways synergistically induces a nuclear GAPDH-dependent cell death. PMID: 22964641
41. The data presented demonstrate that up-regulation of GAPDH positively associated genes is proportional to the malignant stage of various tumors and is associated with an unfavourable prognosis. PMID: 23620736
42. In a yeast two-hybrid screen of a heart cDNA library with Mst1 as bait, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as an Mst1-interacting protein. PMID: 23527007
43. interaction between prolyl oligopeptidase and glyceraldehyde-3-phosphate dehydrogenase is required for cytosine arabinoside-induced glyceraldehyde-3-phosphate dehydrogenase nuclear translocation and cell death PMID: 23348613
44. NleB, a bacterial glycosyltransferase, targets GAPDH function to inhibit NF-kappaB activation. PMID: 23332158
45. GAPDH binds to alkylated, single-stranded, double-stranded and telomeric sequences in a drug-dependent and DNA sequence/structure-dependent manner. PMID: 23409959
46. GAPDH negatively regulates HIV-1 infection and provide insights into a novel function of GAPDH in the HIV-1 life cycle and a new host defense mechanism against HIV-1 infection. PMID: 23237566
47. The strength, selectivity, reversibility, and redox sensitivity of heme binding to GAPDH are consistent with it performing heme sensing or heme chaperone-like functions in cells. PMID: 22957700
48. The ability of C1q to sense both human and bacterial GAPDHs sheds new insights on the role of this important defense collagen molecule in modulating the immune response. PMID: 23086952
49. SIRT1 functions to retain GAPDH in the cytosol, protecting the enzyme from nuclear translocation via interaction with these two proteins. PMID: 22789853
50. The present mini review summarizes recent findings relating to the extraglycolytic functions of GAPDH and highlights the significant role this enzyme plays in regulating both cell survival and apoptotic death--{REVIEW} PMID: 21895736
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亞細胞定位：

Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton.
蛋白家族：

Glyceraldehyde-3-phosphate dehydrogenase family
數據庫鏈接：

HGNC: 4141

OMIM: 138400

KEGG: hsa:2597

STRING: 9606.ENSP00000229239

UniGene: Hs.544577

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