His-Tag Monoclonal Antibody
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中文名稱：His-Tag鼠單克隆抗體
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貨號(hào)：CSB-MA000159
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規(guī)格：￥1090
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圖片：
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IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with His-tag fusion protein
Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
Lane 6: Recombinant protein (E.coli) -
IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
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2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號(hào)：CSB-MA000159 His-Tag單克隆抗體是一款高特異性科研工具，專為檢測(cè)帶有多組氨酸標(biāo)簽（His-Tag）的重組蛋白設(shè)計(jì)。該抗體采用雜交瘤技術(shù)制備，可精準(zhǔn)識(shí)別6×His、10×His等常見組氨酸標(biāo)簽序列，適用于所有物種來源的His標(biāo)簽蛋白檢測(cè)，在Western Blot實(shí)驗(yàn)中能清晰呈現(xiàn)目標(biāo)條帶，免疫熒光檢測(cè)中可實(shí)現(xiàn)精準(zhǔn)定位，同時(shí)支持免疫沉淀技術(shù)用于蛋白互作研究及ELISA定量分析。其廣譜反應(yīng)性特點(diǎn)使其成為重組蛋白表達(dá)驗(yàn)證、純化工藝優(yōu)化及功能研究中的核心試劑，尤其適用于大腸桿菌、哺乳動(dòng)物細(xì)胞等表達(dá)系統(tǒng)的蛋白檢測(cè)。產(chǎn)品經(jīng)嚴(yán)格質(zhì)控確保批次穩(wěn)定性，滿足多場(chǎng)景科研需求，為蛋白質(zhì)組學(xué)、分子互作研究及生物制藥開發(fā)提供可靠支持。關(guān)鍵詞：His-Tag單克隆抗體、重組蛋白檢測(cè)、Western Blot抗體、免疫沉淀試劑、組氨酸標(biāo)簽檢測(cè)。
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宿主：Mouse
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反應(yīng)種屬：N/A
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免疫原：Synthetic Peptide
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標(biāo)記方式：Non-conjugated
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抗體亞型：IgG
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克隆號(hào)：8F7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻(xiàn)
- Shank3 ameliorates neuronal injury after cerebral ischemia/reperfusion via inhibiting oxidative stress and inflammation H Zhang,Redox biology,2024
- When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell Siqueira RAGB, et al,Toxicon,2019
- Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus Huang D, et al,Nature Plants,2018
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