His Monoclonal Antibody
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中文名稱：His鼠單克隆抗體
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貨號：CSB-MA000011M1m
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規格：￥400
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圖片：
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Western Blot
Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4 KDa
Observed band size: 43.4 KDa
Exposure time：5min -
Western Blot
Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:62.5 KDa
Observed band size: 66 KDa
Exposure time：5min -
Western Blot
Positive WB detected in:200ng of four Recombinant protein with his tag All lanes: His antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4, 62.5,56,80.6 KDa
Observed band size:43.4, 66, 57 ,90 KDa
Exposure time：5min -
Immunoprecipitating His in 293 whole cell lysate transfected with His
Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. Lane 2: 2F4B5 antibody (2μg) + 293 whole cell lysate transfected with His (500μg)
Lane 3: 293 whole cell lysate transfected with His (5μg)
For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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其他：
產品詳情
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產品描述：該His Tag單克隆抗體（CUSABIO貨號：CSB-MA000011M1m）專為高效檢測重組蛋白中廣泛應用的6×組氨酸標簽設計，采用雜交瘤技術制備確保批次穩定性，在Western Blot實驗中可適應1:1000至1:640,000的寬泛稀釋范圍，免疫沉淀實驗僅需2-5μL即可有效捕獲目標蛋白。該抗體具有優異的種屬兼容性，經嚴格驗證適用于ELISA、WB、IP三大核心實驗體系，特別適合重組表達蛋白的定性定量分析、蛋白相互作用研究及高通量篩選等科研場景。其高親和力特性可精準識別His標簽蛋白，在變性或天然條件下均保持穩定結合能力，為蛋白質純化工藝開發、表達驗證及功能研究提供可靠工具。作為His標簽檢測領域的優選試劑，該產品憑借靈敏的檢測限和低交叉反應性特點，廣泛應用于分子生物學、結構生物學及生物制藥相關的基礎研究中。
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別名：6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
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宿主：Mouse
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反應種屬：All
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免疫原：The immunogen is a mixture of various His-tagged proteins
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG1
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純化方式：>95%, Protein G purified
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克隆號：2F4B5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:640000 IP 2μl-5μl -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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