DDB2 Recombinant Monoclonal Antibody

說明書

中文名稱：

DDB2 Recombinant Monoclonal Antibody
貨號：

CSB-RA062911A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of DDB2 expression in HT-1080 cells using DDB2 antibody. Green, isotype control; red, DDB2.

Immunocytochemical staining of HT-1080 cells with DDB2 antibody. Nuclei were stained blue with DAPI; DDB2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using DDB2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q92466
基因名：

DDB2
別名：

DDB2; Damage Specific DNA Binding Protein 2; UV-Damaged DNA-Binding Protein 2; DDB P48 Subunit; UV-DDB2; DDBB; XPE; Xeroderma Pigmentosum Group E Protein; DNA Damage-Binding Protein 2; FLJ34321; Damage-Specific DNA Binding Protein 2 (48kD); Damage-Specific DNA Binding Protein 2, 48kDa; Damage-Specific DNA-Binding Protein 2; UV-DDB; DDBb
反應種屬：

Human, Mouse
免疫原：

Recombinant Human DDB2 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1E10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR.; Inhibits UV-damaged DNA repair.; Inhibits UV-damaged DNA repair.
基因功能參考文獻：
1. Study identified novel mutation DDB2 p. (Lys381Argfs*2) present at a homozygous state in 5 affected cases belonging to the same Tunisian family with Xeroderma pigmentosum. PMID: 29169765
2. Chronic low-dose of UVB (CLUV) treatment activates p53, which corroborate with the increased level of DDB2 and XPC proteins. DDB2 and XPC recruited at chromatin bound, suggesting a more efficient cyclobutane pyrimidine dimer (CPD) recognition by NER and more efficient repair of CDP. PMID: 29448173
3. DDB2-PCNA interaction may contribute to a correct DNA damage response for maintaining genome integrity PMID: 29604309
4. DDB2 is modified by SUMOylation upon ultraviolet irradiation, and this post-translational modification plays an important role in the initial recognition and processing of ultraviolet irradiation-induced DNA damage occurring within the context of chromatin. PMID: 28981631
5. Results show that DDB2 is critical for chromatin association of XRCC5/6 in the absence of DNA damage and provide evidence that XRCC5/6 are functional partners of DDB2 in its transcriptional stimulatory activity. PMID: 28035050
6. UVRAG is a regulator of CRL4(DDB2)-mediated nucleotide excision repair and its expression levels may influence melanoma predisposition. PMID: 27203177
7. High DDB2 expression is associated with increased radioresistance of non-small cell lung cancer. PMID: 27553023
8. data revealed that DDB2 is involved in early events occurring during metastatic progression of breast cancer cells and will contribute to define this protein as a new marker of metastatic progression in this type of cancer. PMID: 26879405
9. DDB2 polymorphisms are associated with gastric cancer and atrophic gastritis risks. PMID: 26760766
10. the release of NER components such as DNA damage binding protein 2 (DDB2) and Xeroderma Pigmentosum complementation group C protein (XPC) following oxidative stress might putatively involve their apoptotic role rather than DNA repair function. PMID: 26263968
11. PKM2 interacts with DDB2 and reduces cell survival upon UV irradiation. PMID: 26410533
12. DDB2 can bind to the promoter region of NEDD4L and recruit enhancer of zeste homolog 2 histone methyltransferase to repress NEDD4L transcription by enhancing histone H3 lysine 27 trimethylation at the NEDD4L promoter. PMID: 26130719
13. data demonstrated that the DDB2 IRES activity was promoted during stress conditions. These results reveal a novel mechanism contributing to DDB2 expression PMID: 26187069
14. DDB2 is involved in ubiquitination and degradation of PAQR3 in gastric cancer cells. PMID: 26205499
15. DDB2 rs747650 is involved in androgen metabolism, inflammation processes and scar formation in severe acne. PMID: 24399259
16. DDB2 plays an instrumental role in DNA damage induced ROS accumulation, ROS induced premature senescence and inhibition of skin tumorigenesis. PMID: 23109835
17. DDB2 is protected by XPC from ubiquitination and degradation in a stochastic manner; thus XPC allows DDB2 to initiate multiple rounds of repair events, thereby contributing to the persistence of cellular DNA repair capacity PMID: 25628365
18. DDB2 protein expression is low in ovarian tumor cells. PMID: 24574518
19. Chromatin retention of DNA damage sensors DDB2 and XPC through loss of p97 segregase causes genotoxicity. PMID: 24770583
20. DDB2 is a PCNA-binding protein and this association is required for DDB2 proteolytic degradation. PMID: 24200966
21. study provides new knowledge about the posttranslational regulation of DDB2 and expands the biological functions of protein alpha-N-methylation to DNA repair PMID: 24753253
22. the data indicate that p53-dependent upregulation of XPC and DDB2 is a key mechanism upon genotoxic stress, whereby melanoma cells acquire resistance towards DNA cross-linking agents. PMID: 23604128
23. DDB2 and a DDB2-ATM feedback loop influence HCMV replication. PMID: 24335308
24. Knockdown of DDB2-induced IkappaBalpha gene expression restored NF-kappaB activity. PMID: 23774208
25. These results suggest that the SUMOylation of DDB2 facilitates CPD repair. PMID: 23860269
26. DDB2 facilitates ATR and ATM recruitment to the DNA damage site following UV irradiation. PMID: 23422745
27. Results indicate a transcriptional regulatory pathway of DDB2 that is directly linked to the mechanisms that suppress metastasis of colon cancer. PMID: 23610444
28. The 3'untranslated region of DDB2 contains a cis-acting element that affects stability, export and translation of the message PMID: 23605047
29. Data indicate that poly(ADP-ribose) polymerase-1 (PARP-1)collaborates with DNA-binding protein 2 (DDB2) to increase the efficiency of the lesion recognition step of global genomic subpathway of NER (GG-NER). PMID: 23319653
30. DDB2 can inhibit cell growth rate in AR-expressing cells (LNCaP) but not in AR-null cells (PC3). PMID: 22846800
31. data support a model in which poly(ADP-ribosyl)ation of DDB2 suppresses DDB2 ubiquitylation and outline a molecular mechanism for PARP1-mediated regulation of nucleotide excision repair through DDB2 stabilization and recruitment of the chromatin remodeler ALC1 PMID: 23045548
32. study reports a new function of DDB2 in modulating chromatin structure at DNA lesions PMID: 22492724
33. DDB2 subunit of UV-DDB associates transiently with the DNA-binding domain of XPC to fine-tune its engagement with CPD lesions PMID: 22039351
34. Nucleotide excision repair proteins rapidly accumulate but fail to persist in human xeroderma pigmentosum XP-E (DDB2 mutant) cells. PMID: 21388382
35. Multiple skin cancers in adults are associted with mutations in the XP-E (DDB2) DNA repair gene. PMID: 21107348
36. Overexpression of DDB2 enhances the sensitivity of ovarian cancer cells to cisplatin by augmenting cellular apoptosis. PMID: 20013802
37. Damaged DNA-binding protein 2 (DDB2) protects against UV irradiation in human cells and Drosophila PMID: 20398405
38. Data show that XPC and Ku oppositely regulate the ubiquitin ligase activity of DDB2, and that DDB2 complex-mediated ubiquitylation plays a role in recruiting XPA to damaged sites. PMID: 20368362
39. These results indicate that DDB2 is a modulator of UV-induced apoptosis, and that UV resistance can be overcome by inhibition of DDB2 PMID: 11852074
40. These results demonstrate direct activation of the human DDB2 gene by p53. The corresponding region in the mouse DDB2 gene shared significant sequence identity with the human gene but was deficient for p53 binding and transcriptional activation. PMID: 11971958
41. BRCA1 upregulates DDB2, with some evidence that p53 is involved in its regulation. PMID: 12496474
42. overexpression of DDB2 in V79 cell potentiates DNA repair and protects cells from UV-induced apoptosis and cytotoxicity. PMID: 12553360
43. DDB2 and CSA are each integrated into nearly identical complexes via interaction with DDB1 PMID: 12732143
44. DDB2p48 activates the recruitment of XPC to cyclobutane pyrimidine dimers and may be the initial recognition factor in the nucleotide excision repair pathway PMID: 12944386
45. Data suggest that both before and after UV irradiation, DDB2 directly regulates p53 levels, while DDB2 expression is itself regulated by p53. PMID: 14560002
46. identification of four DDB2 variants from HeLa cells (D1-D4) that are generated by alternative splicing PMID: 14751237
47. DDB2 regulates TNF signaling-mediated apoptosis via cFLIP and contributes to acquired cross-resistance. PMID: 15644494
48. UV-DDB interacts with XPC physically, and both are polyubiquitylated by the UV-DDB-ubiquitin ligase complex. PMID: 15882621
49. DDB2 has an intrinsic damaged DNA binding activity PMID: 16260596
50. CUL-4A mediates the proteolytic degradation of DDB2 and this degradation event, initiated at the lesion sites, regulates damage recognition by XPC. PMID: 16527807
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相關疾病：

Xeroderma pigmentosum complementation group E (XP-E)
亞細胞定位：

Nucleus. Note=Accumulates at sites of DNA damage following UV irradiation.
蛋白家族：

WD repeat DDB2/WDR76 family
組織特異性：

Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.
數據庫鏈接：

HGNC: 2718

OMIM: 278740

KEGG: hsa:1643

STRING: 9606.ENSP00000256996

UniGene: Hs.700338

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