DDB2 Recombinant Monoclonal Antibody
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中文名稱：DDB2重組抗體
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貨號：CSB-RA442393A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: PC-3 whole cell lysate
All lanes: DDB2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
IHC image of CSB-RA442393A0HU diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.46% DAB. -
IHC image of CSB-RA442393A0HU diluted at 1:50 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.46% DAB. -
Overlay Peak curve showing A549 cells stained with CSB-RA442393A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：CSB-RA442393A0HU DDB2重組單克隆抗體是經ELISA、WB、IHC和FC多平臺驗證的高特異性科研工具，適用于檢測DNA損傷結合蛋白2（DDB2）的表達及分布。該抗體針對DDB2蛋白設計，其靶點作為損傷特異性DNA結合復合體核心組分，在核苷酸切除修復通路中發揮關鍵作用，參與識別紫外線誘導的DNA損傷，并在氧化應激響應及細胞周期調控中具有重要功能。實驗數據顯示，在WB檢測中抗體呈現清晰的單一特異性條帶，IHC實驗可準確顯示組織樣本中DDB2的亞細胞定位，流式細胞分析（FC）能有效檢測細胞內源蛋白表達水平，推薦使用濃度分別為WB 1:500-1:2000、IHC 1:50-1:200、FC 1:50-1:200。本產品適用于DNA損傷修復機制研究、氧化應激相關信號通路探索、腫瘤發生發展中基因組穩定性分析，以及環境毒理或輻射生物學領域的蛋白質功能研究，為體外實驗及模型生物樣本分析提供可靠檢測工具。該產品嚴格限于科研用途。
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Uniprot No.：
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基因名：
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別名：DNA damage-binding protein 2 (DDB p48 subunit) (DDBb) (Damage-specific DNA-binding protein 2) (UV-damaged DNA-binding protein 2) (UV-DDB 2), DDB2
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human DDB2
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：24E5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR.; Inhibits UV-damaged DNA repair.; Inhibits UV-damaged DNA repair.
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基因功能參考文獻：
- Study identified novel mutation DDB2 p. (Lys381Argfs*2) present at a homozygous state in 5 affected cases belonging to the same Tunisian family with Xeroderma pigmentosum. PMID: 29169765
- Chronic low-dose of UVB (CLUV) treatment activates p53, which corroborate with the increased level of DDB2 and XPC proteins. DDB2 and XPC recruited at chromatin bound, suggesting a more efficient cyclobutane pyrimidine dimer (CPD) recognition by NER and more efficient repair of CDP. PMID: 29448173
- DDB2-PCNA interaction may contribute to a correct DNA damage response for maintaining genome integrity PMID: 29604309
- DDB2 is modified by SUMOylation upon ultraviolet irradiation, and this post-translational modification plays an important role in the initial recognition and processing of ultraviolet irradiation-induced DNA damage occurring within the context of chromatin. PMID: 28981631
- Results show that DDB2 is critical for chromatin association of XRCC5/6 in the absence of DNA damage and provide evidence that XRCC5/6 are functional partners of DDB2 in its transcriptional stimulatory activity. PMID: 28035050
- UVRAG is a regulator of CRL4(DDB2)-mediated nucleotide excision repair and its expression levels may influence melanoma predisposition. PMID: 27203177
- High DDB2 expression is associated with increased radioresistance of non-small cell lung cancer. PMID: 27553023
- data revealed that DDB2 is involved in early events occurring during metastatic progression of breast cancer cells and will contribute to define this protein as a new marker of metastatic progression in this type of cancer. PMID: 26879405
- DDB2 polymorphisms are associated with gastric cancer and atrophic gastritis risks. PMID: 26760766
- the release of NER components such as DNA damage binding protein 2 (DDB2) and Xeroderma Pigmentosum complementation group C protein (XPC) following oxidative stress might putatively involve their apoptotic role rather than DNA repair function. PMID: 26263968
- PKM2 interacts with DDB2 and reduces cell survival upon UV irradiation. PMID: 26410533
- DDB2 can bind to the promoter region of NEDD4L and recruit enhancer of zeste homolog 2 histone methyltransferase to repress NEDD4L transcription by enhancing histone H3 lysine 27 trimethylation at the NEDD4L promoter. PMID: 26130719
- data demonstrated that the DDB2 IRES activity was promoted during stress conditions. These results reveal a novel mechanism contributing to DDB2 expression PMID: 26187069
- DDB2 is involved in ubiquitination and degradation of PAQR3 in gastric cancer cells. PMID: 26205499
- DDB2 rs747650 is involved in androgen metabolism, inflammation processes and scar formation in severe acne. PMID: 24399259
- DDB2 plays an instrumental role in DNA damage induced ROS accumulation, ROS induced premature senescence and inhibition of skin tumorigenesis. PMID: 23109835
- DDB2 is protected by XPC from ubiquitination and degradation in a stochastic manner; thus XPC allows DDB2 to initiate multiple rounds of repair events, thereby contributing to the persistence of cellular DNA repair capacity PMID: 25628365
- DDB2 protein expression is low in ovarian tumor cells. PMID: 24574518
- Chromatin retention of DNA damage sensors DDB2 and XPC through loss of p97 segregase causes genotoxicity. PMID: 24770583
- DDB2 is a PCNA-binding protein and this association is required for DDB2 proteolytic degradation. PMID: 24200966
- study provides new knowledge about the posttranslational regulation of DDB2 and expands the biological functions of protein alpha-N-methylation to DNA repair PMID: 24753253
- the data indicate that p53-dependent upregulation of XPC and DDB2 is a key mechanism upon genotoxic stress, whereby melanoma cells acquire resistance towards DNA cross-linking agents. PMID: 23604128
- DDB2 and a DDB2-ATM feedback loop influence HCMV replication. PMID: 24335308
- Knockdown of DDB2-induced IkappaBalpha gene expression restored NF-kappaB activity. PMID: 23774208
- These results suggest that the SUMOylation of DDB2 facilitates CPD repair. PMID: 23860269
- DDB2 facilitates ATR and ATM recruitment to the DNA damage site following UV irradiation. PMID: 23422745
- Results indicate a transcriptional regulatory pathway of DDB2 that is directly linked to the mechanisms that suppress metastasis of colon cancer. PMID: 23610444
- The 3'untranslated region of DDB2 contains a cis-acting element that affects stability, export and translation of the message PMID: 23605047
- Data indicate that poly(ADP-ribose) polymerase-1 (PARP-1)collaborates with DNA-binding protein 2 (DDB2) to increase the efficiency of the lesion recognition step of global genomic subpathway of NER (GG-NER). PMID: 23319653
- DDB2 can inhibit cell growth rate in AR-expressing cells (LNCaP) but not in AR-null cells (PC3). PMID: 22846800
- data support a model in which poly(ADP-ribosyl)ation of DDB2 suppresses DDB2 ubiquitylation and outline a molecular mechanism for PARP1-mediated regulation of nucleotide excision repair through DDB2 stabilization and recruitment of the chromatin remodeler ALC1 PMID: 23045548
- study reports a new function of DDB2 in modulating chromatin structure at DNA lesions PMID: 22492724
- DDB2 subunit of UV-DDB associates transiently with the DNA-binding domain of XPC to fine-tune its engagement with CPD lesions PMID: 22039351
- Nucleotide excision repair proteins rapidly accumulate but fail to persist in human xeroderma pigmentosum XP-E (DDB2 mutant) cells. PMID: 21388382
- Multiple skin cancers in adults are associted with mutations in the XP-E (DDB2) DNA repair gene. PMID: 21107348
- Overexpression of DDB2 enhances the sensitivity of ovarian cancer cells to cisplatin by augmenting cellular apoptosis. PMID: 20013802
- Damaged DNA-binding protein 2 (DDB2) protects against UV irradiation in human cells and Drosophila PMID: 20398405
- Data show that XPC and Ku oppositely regulate the ubiquitin ligase activity of DDB2, and that DDB2 complex-mediated ubiquitylation plays a role in recruiting XPA to damaged sites. PMID: 20368362
- These results indicate that DDB2 is a modulator of UV-induced apoptosis, and that UV resistance can be overcome by inhibition of DDB2 PMID: 11852074
- These results demonstrate direct activation of the human DDB2 gene by p53. The corresponding region in the mouse DDB2 gene shared significant sequence identity with the human gene but was deficient for p53 binding and transcriptional activation. PMID: 11971958
- BRCA1 upregulates DDB2, with some evidence that p53 is involved in its regulation. PMID: 12496474
- overexpression of DDB2 in V79 cell potentiates DNA repair and protects cells from UV-induced apoptosis and cytotoxicity. PMID: 12553360
- DDB2 and CSA are each integrated into nearly identical complexes via interaction with DDB1 PMID: 12732143
- DDB2p48 activates the recruitment of XPC to cyclobutane pyrimidine dimers and may be the initial recognition factor in the nucleotide excision repair pathway PMID: 12944386
- Data suggest that both before and after UV irradiation, DDB2 directly regulates p53 levels, while DDB2 expression is itself regulated by p53. PMID: 14560002
- identification of four DDB2 variants from HeLa cells (D1-D4) that are generated by alternative splicing PMID: 14751237
- DDB2 regulates TNF signaling-mediated apoptosis via cFLIP and contributes to acquired cross-resistance. PMID: 15644494
- UV-DDB interacts with XPC physically, and both are polyubiquitylated by the UV-DDB-ubiquitin ligase complex. PMID: 15882621
- DDB2 has an intrinsic damaged DNA binding activity PMID: 16260596
- CUL-4A mediates the proteolytic degradation of DDB2 and this degradation event, initiated at the lesion sites, regulates damage recognition by XPC. PMID: 16527807
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相關疾病：Xeroderma pigmentosum complementation group E (XP-E)
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亞細胞定位：Nucleus. Note=Accumulates at sites of DNA damage following UV irradiation.
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蛋白家族：WD repeat DDB2/WDR76 family
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組織特異性：Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.
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