XPNPEP1 Recombinant Monoclonal Antibody

說明書

中文名稱：

XPNPEP1重組抗體
貨號：

CSB-RA878859MA1HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA878859MA1HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing JK cells stained with CSB-RA878859MA1HU (red line) at 1:300. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,005 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA878859MA1HU XPNPEP1重組單克隆抗體是針對氨肽酶P（XPNPEP1）靶點開發(fā)的高特異性科研工具。該抗體通過重組技術(shù)制備，具有批次穩(wěn)定性和高親和力，經(jīng)ELISA、免疫組化（IHC）及流式細(xì)胞術(shù)（FC）多平臺驗證，在推薦稀釋比例范圍內(nèi)（IHC 1:20-1:200，F(xiàn)C 1:20-1:200）表現(xiàn)出優(yōu)異的目標(biāo)蛋白結(jié)合能力。XPNPEP1作為細(xì)胞外基質(zhì)調(diào)節(jié)酶，參與血管緊張素、緩激肽等活性肽的代謝調(diào)控，在心血管穩(wěn)態(tài)和腎臟功能研究中具有重要價值。本產(chǎn)品適用于體外培養(yǎng)細(xì)胞或組織樣本中XPNPEP1的定位檢測及表達(dá)水平分析，可支持血管生物學(xué)、代謝性疾病機(jī)制、藥物靶點篩選等基礎(chǔ)研究領(lǐng)域。其單克隆特性確保實驗數(shù)據(jù)的高度可重復(fù)性，特別適用于需要長期追蹤蛋白動態(tài)變化的實驗體系，為探索XPNPEP1在細(xì)胞信號傳導(dǎo)及病理生理過程中的功能提供可靠工具。
Uniprot No.：

Q9NQW7
基因名：

XPNPEP1
別名：

Aminoacylproline aminopeptidase antibody; aminopeptidase P, cytosolic antibody; APP1 antibody; Cytosolic aminopeptidase P antibody; RP11 451M19.1 antibody; sAmp antibody; Soluble aminopeptidase P antibody; soluble antibody; X Pro aminopeptidase 1 antibody; X prolyl aminopeptidase (aminopeptidase P) 1 antibody; X prolyl aminopeptidase (Aminopeptidase P) 1 soluble antibody; X prolyl aminopeptidase 1 antibody; X prolyl aminopeptidase 1 soluble antibody; X-Pro aminopeptidase 1 antibody; X-prolyl aminopeptidase 1 antibody; Xaa Pro aminopeptidase 1 antibody; Xaa-Pro aminopeptidase 1 antibody; XPNPEP 1 antibody; XPNPEP antibody; xpnpep1 antibody; XPNPEPL antibody; XPNPEPL1 antibody; XPP1_HUMAN antibody
反應(yīng)種屬：

Human
免疫原：

Recombinant Human XPNPEP1 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse IgG
純化方式：

Affinity-chromatography
克隆號：

4D12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro.
基因功能參考文獻(xiàn)：
1. Alanine replacement of Arg535 destabilizes the AMPP dimer and guanidine hydrochloride restores the native monomer-dimer equilibrium. It is proposed that Arg535 plays an important role in AMMP catalysis and in stabilization of the catalytically active dimeric state PMID: 29351301
2. These data suggest that progesterone-induced increases in AP-P may contribute to the development of oral contraceptive pill-induced hypertension in susceptible Women. PMID: 19126663
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Peptidase M24B family
組織特異性：

Expressed in all tissues tested, including pancreas, heart, muscle, kidney, liver, lung and brain. Highest levels in pancreas.
數(shù)據(jù)庫鏈接：

HGNC: 12822

OMIM: 602443

KEGG: hsa:7511

STRING: 9606.ENSP00000421566

UniGene: Hs.390623

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