LGR5 Recombinant Monoclonal Antibody

說明書

中文名稱：

LGR5重組抗體
貨號：

CSB-RA262034A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: LGR5 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 100, 98, 93 kDa
Observed band size: 100 kDa

Overlay histogram showing HepG2 cells stained with CSB-RA262034A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA262034A0HU LGR5重組單克隆抗體是針對人源富含亮氨酸重復的G蛋白偶聯受體5（LGR5）開發的高特異性科研工具，該靶點作為腸道干細胞和多種成體干細胞的標志物，在組織再生及腫瘤發生機制研究中具有重要價值。本產品經嚴格驗證可應用于ELISA、Western Blot及流式細胞術（FC）三大實驗平臺，其中Western Blot檢測顯示清晰的單一條帶，流式細胞分析呈現特異性膜表面結合信號，推薦使用濃度分別為WB 1:500-1:5000、FC 1:20-1:200。其重組單克隆特性確保了批次間的高度一致性，適用于干細胞標記追蹤、腫瘤干細胞分群鑒定、類器官培養體系監測等前沿研究領域，為探索組織穩態維持、癌癥演進機制及再生醫學調控通路提供可靠檢測方案。
Uniprot No.：

O75473
基因名：

LGR5
別名：

Leucine-rich repeat-containing G-protein coupled receptor 5 (G-protein coupled receptor 49) (G-protein coupled receptor 67) (G-protein coupled receptor HG38), LGR5, GPR49 GPR67
反應種屬：

Human, Rat
免疫原：

A synthesized peptide derived from human LGR5/GPR49
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4H8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
基因功能參考文獻：
1. Data conclude that LGR5 expression in CTCs may serve as a marker for CRC metastasis. PMID: 29949050
2. LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/beta-catenin pathway and would thus be a novel therapeutic target for glioma. PMID: 30208924
3. Data found LGR5 as a potential target of miR-340. LGR5 was highly expressed in breast cancer cells. Relative expression of LGR5 was negatively regulated by miR-340. Knockdown of LGR5 also inhibited cell proliferation and drug resistance to docetaxel with enhanced cell apoptosis of breast cancer cells. PMID: 30300682
4. knockdown of CASC15 significantly inhibited the proliferation, migration and invasion of colon cancer cells in vitro and in vivo. Following mechanistic experiments, CASC15 was observed to act as a sponge to suppress microRNA (miR)4310 that targeted LGR5. PMID: 29956772
5. LGR5 overexpression was associated with high T stage and LN metastasis status in breast cancer. High LGR5 expression was also associated with reduced relapse-free survival, indicating that LGR5 may represent a promising prognostic marker for breast cancer patients. PMID: 29471794
6. LGR5 is a critical effector of DDX1 in colorectal cancer cells. PMID: 29869821
7. Logarithmic expansion of LGR5-expressing cells was observed in human colorectal cancer. PMID: 28958617
8. Our findings indicated that the Lgr5High/DCLK1High expression pattern may be considered as a signature phenotype for intestinal subtypes of gastric carcinoma. PMID: 28946555
9. Findings suggest a tumor suppressor role for miR-216a in gliomas, which inhibits glioma cell proliferation, migration, and invasion by targeting LGR5. PMID: 28256193
10. LGR5 promotes cancer stem cell traits and chemoresistance in cervical cancer. PMID: 28880275
11. LGR5 expression in the epithelium and stroma was associated with tumor stage, and by integrating functional experiments with LGR5-sorted cell RNA sequencing data from adenoma and normal organoids, there were correlations between LGR5 and CRC-specific genes, including dickkopf WNT signaling pathway inhibitor 4 (DKK4) and SPARC-related modular calcium binding 2 (SMOC2 PMID: 29467240
12. LGR5 expression is transiently upregulated during the early stage of cardiomyocyte differentiation from hPSCs, and although LGR5 expression is not required for maintaining hPSCs in the undifferentiated state, knockdown of LGR5 leads to decreased expression of key cardiac transcription factors at the early stage with eventual lack of robust beating cardiomyocytes at the late stage. PMID: 28793247
13. Lgr5 plays important roles in angiogenesis. Lgr5-specific siRNA could be designed into an effective therapeutic agent to inhibit gastric cancer angiogenesis. PMID: 28404940
14. A regulatory mechanism of LGR5 expression in gastric carcinogenesis, with SP1 as an important component of the transcriptional complex and LGR5 activity, which is modulated by its ligands RSPO1 and RSPO2, whose expression is modulated by methylation. PMID: 28219935
15. these results demonstrate that Lgr5 activation of Wnt/beta-catenin signaling is a potential mechanism to promote the progression of ESCC and ESCC stem cell renewal, and Lgr5 may be used as a molecular target for the development of treatments for ESCC. PMID: 28404917
16. This study uncovers a novel cross-talk between LGR5 and TGFbeta signaling in colon cancer and identifies LGR5 as a new modulator of TGFbeta signaling able to suppress colon cancer metastasis. PMID: 28939678
17. Data show that IKKalpha directly bind to the promoters of LGR5, in turn, upregulating LGR5 expression through activation of STAT3 signaling pathway during cancer progression. PMID: 27049829
18. Report the presence of aberrantly located LGR5(+) cells coexpressing epithelial markers in the stromal compartment of women with endometriosis. These cells have a statistically significantly different expression profile in deep-infiltrating endometriosis in comparison with other types of endometriosis, independent of the menstrual cycle phase. PMID: 28923287
19. Arrb1 reduced the chemotherapy-induced Lgr5 stem cell apoptosis by inhibiting endoplasmic reticulum stress-mediated mitochondrial apoptotic signaling. PMID: 27195676
20. LGR5 signaling in gastric cancer cells.LGR5 crosstalk with FOXO1 in gastric cancer cells. PMID: 28970066
21. High LGR5 expression is associated with osteoarthritis. PMID: 28777797
22. The LGR5 primarily functions via the IQGAP1-Rac1 pathway to strengthen cell-cell adhesion in normal adult crypt stem cells and colon cancer cells. PMID: 28739799
23. Lgr5(+) chief cells are involved in maintaining the homeostatic stem cell pool. Define Lgr5(+) chief cells as a major cell-of-origin of gastric cancer. PMID: 28581476
24. LGR5-expressing fraction of CD54+ cells represents gastric cancer CSCs, in which LGR5 is closely associated with stemness and EMT core genes PMID: 28033430
25. clathrin-mediated endocytosis inhibition of the leucine-rich G protein-coupled receptor-5 diminishes cell fitness PMID: 28275053
26. The findings indicated that Lgr5 might contribute to the intestinal metaplasia during gastric carcinogenesis. PMID: 26077638
27. Endometrial LGR5 is not an endogenous stem cell marker. Instead, LGR5(+) cells appear to be recruited from blood to be part of the stem cell niche at the perivascular microenvironment to activate the endogenous niche. PMID: 27887719
28. 2 LGR5 splice variants were expressed in human intestine crypt cells; one lacked exon 5 and the other lacked exons 5-8. Only LGR5FL appeared during cell cycle arrest. The transcript variants appeared when the cell cycle was proceeding. LGR5FL-positive cells were negative for Ki-67 and were enriched after chemotherapy. PMID: 27140312
29. Low expression of LGR5 is associated with gastric cancer. PMID: 26386561
30. Report targeting LGR5+ tumor stem cells with an antibody-drug conjugate as effective treatment for colon cancer. PMID: 26582901
31. Lgr5 overexpression was significantly associated with deep invasion of colorectal cancer lymphnode metastasis, distant metastasis and AJCC stage PMID: 26758198
32. Data suggest that targeting of leucine rich repeat containing G protein-coupled receptor 5 (LGR5) may be of therapeutic benefit for neuroblastomas. PMID: 26517508
33. This study shows that Lgr5 can be a valuable and reliable prognostic factor of colorectal cancer progression. PMID: 26674601
34. We conclude that overexpression of LGR5 is associated with markers of tumor aggressiveness in human papillary thyroid cancer PMID: 26416247
35. The results suggest that trichosanthin may induce apoptosis in glioma cells by targeting LGR5 and repressing the Wnt/betabcatenin signaling pathway PMID: 26397053
36. These findings indicate that LGR5 not only participates in carcinogenesis but also maintained stemness by activating Wnt/b-catenin signaling in breast cancer. PMID: 26086949
37. We confirmed in a large and independent study cohort that LGR5 rs17109924 is a predictive genetic biomarker for time to recurrence in patients with colon cancer treated with 5-FU-based adjuvant chemotherapy. PMID: 25665511
38. lgr5 methylation, through the regulation of lgr5 expression and colorectal CSC differentiation, may constitute a novel prognostic marker for colorectal cancer patients. PMID: 26599100
39. High resolution crystal structure of an ectodomain variant of human LGR5 (hLGR5ecto) complexed with a signalling competent fragment of mouse Rspo2. PMID: 26123262
40. Lgr5-positive cells may be cancer stem cells-like cells in gastric cancer. PMID: 26279446
41. Demonstrate the presence of LGR5-positive cells in limbal epithelial crypts and their decrease in inflamed conditions, which suggests a critical role of this protein during inflammation and its possible use as a marker in normal crypts. PMID: 26097379
42. Elevated Lgr5 expression might contribute to the development and progression of colorectal cancer. PMID: 24751002
43. data reveal a RSPO2-induced, LGR5-dependent Wnt signaling-negative feedback loop that exerts a net growth-suppressive effect on CRC cells PMID: 24476626
44. our data highlight a potential role of Lgr5-positive cells in the tumorigenesis of colorectal cancer (CRC), and suggest that treating these Lgr5-positive cells in CRCs may substantially improve the outcome of CRC therapy PMID: 25835970
45. Lgr5 may play an important role in the development and progression of cervical carcinoma. PMID: 25973068
46. Based on the lack of correlation between Lgr5 and tested cancer stem cell markers, Lgr5 does not seem to be a potential stemness marker or prognostic factor in pancreatic ductal adenocarcinoma. PMID: 25804119
47. High LGR5 expression was associated with poor prognosis in patients with colorectal cancer(CRC) and that LGR5 is an efficient prognostic factor in CRC. [meta-analysis; review] PMID: 25192390
48. we suggest that Musashi-1, Lgr5, and pEGFR are overexpressed in human SIAs and may play roles in human SIA carcinogenesis and progression. PMID: 25773390
49. the higher expression level of DCLK1 in patients undergoing CRT can propose it as a more relevant candidate among CSC markers comparing to Lgr5 for colorectal cancer patients. PMID: 25631749
50. increased expression of LGR5 during embryogenesis and the neonatal period alter skin development and homeostasis. PMID: 26003047
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of th
數據庫鏈接：

HGNC: 4504

OMIM: 606667

KEGG: hsa:8549

STRING: 9606.ENSP00000266674

UniGene: Hs.658889

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