LGR5
LGR5（富含亮氨酸重復(fù)序列的G蛋白偶聯(lián)受體5）是Wnt信號(hào)通路的關(guān)鍵受體，又稱G蛋白偶聯(lián)受體49（GPR49），在腸道、毛囊等組織的干細(xì)胞中特異性表達(dá)，被視為成體干細(xì)胞的標(biāo)志物。LGR5通過與R-spondin家族蛋白結(jié)合，增強(qiáng)β-連環(huán)蛋白的穩(wěn)定性，調(diào)控干細(xì)胞自我更新與組織再生。LGR5在結(jié)直腸癌、胃癌等惡性腫瘤中高表達(dá)，其陽性細(xì)胞群體常表現(xiàn)出腫瘤干細(xì)胞特性，與化療耐藥、復(fù)發(fā)轉(zhuǎn)移密切相關(guān)。近年來針對(duì)LGR5的藥物開發(fā)聚焦于單克隆抗體和小分子抑制劑，其中LGR5-ADC（抗體藥物偶聯(lián)物）在臨床前研究中顯示出靶向清除腫瘤干細(xì)胞的潛力。基于LGR5陽性細(xì)胞的CAR-T療法也在探索中，但需克服靶向正常干細(xì)胞的脫靶風(fēng)險(xiǎn)。在再生醫(yī)學(xué)領(lǐng)域，調(diào)控LGR5信號(hào)通路為潰瘍性結(jié)腸炎、放射性腸損傷等疾病提供了新思路。
熱銷產(chǎn)品
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
LGR5 Recombinant Monoclonal Antibody (CSB-RA262034A0HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: LGR5 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 100, 98, 93 kDa
Observed band size: 100 kDa
Overlay histogram showing HepG2 cells stained with CSB-RA262034A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
LGR5 Antibodies
LGR5 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA622827 | LGR5 Antibody | Human,Mouse | ELISA,WB |
| CSB-PA080695 | LGR5 Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-PA012906LA01HU | LGR5 Antibody | Human | ELISA, IHC, IF |
| CSB-PA012906LB01HU | LGR5 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA012906LC01HU | LGR5 Antibody, FITC conjugated | Human | |
| CSB-PA012906LD01HU | LGR5 Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA262034A0HU | LGR5 Recombinant Monoclonal Antibody | Human, Rat | ELISA, WB, FC |
LGR5 Proteins
LGR5 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP012906HU | Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), partial | Yeast |
| CSB-EP012906HU | Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), partial | E.coli |
| Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), partial | ||
| CSB-BP012906HU | Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), partial | Baculovirus |
| CSB-EP012906HUa0 | Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), partial | E.coli |
| CSB-MP012906HU1 | Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), partial | Mammalian cell |
| CSB-EP012906HU-B | Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), partial, Biotinylated | E.coli |
