PYGO2 Recombinant Monoclonal Antibody

中文名稱：

PYGO2 Recombinant Monoclonal Antibody
貨號(hào)：

CSB-RA989447A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA989447A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue上腺 performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA989447A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

Q9BRQ0
基因名：

PYGO2
別名：

1190004M21Rik antibody; FLJ33226 antibody; PP7910 antibody; PYGO2 antibody; Pygo2 protein antibody; PYGO2_HUMAN antibody; Pygopus 2 antibody; pygopus homolog 2 (Drosophila) antibody; Pygopus homolog 2 antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide from human PYGO2 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

10D11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Involved in signal transduction through the Wnt pathway.
基因功能參考文獻(xiàn)：
1. Elevated PYGO2 expression in primary prostate adenocarcinoma is a potential risk factor for biochemical recurrence. PMID: 28924059
2. Overexpression of Pygo2 facilitated the expression of P-glycoprotein, which acts as a drug efflux pump, by promoting the transcription of MDR1 at the MDR1 promoter loci, resulting in acceleration of the efflux of paclitaxel in human glioma cells. PMID: 28427190
3. We also determined the effect of Pygo2 on the sensitivity of breast tumors resistant to doxorubicin in a mouse model. Finally, RNA samples from 64 paired patient tumors (before and after chemotherapy) highly and significantly overexpressed Pygo2 and/or MDR1 after treatment, thus underlining a pivotal role for the Pygo2-mediated Wnt/b-catenin pathway in the clinical chemoresistance of breast cancer. PMID: 26876203
4. Findings are consistent with a model in which acetylation of Pygo2 by CBP/p300 family members in the active TCF/beta-catenin complex occurs coincident with histone acetylation and may be required for the recycling of Pygo2 away from the complex subsequent to target gene activation. PMID: 27647933
5. The activation of its expression by ERalpha and/or specificity protein-1 (SP1) suggests hPYGO2 as a theranostic target for hormone therapy responsive and refractory breast cancer. PMID: 26645832
6. Pygo2 functions as a prognostic factor for glioma due to its up-regulation of H3K4me3 and promotion of MLL1/MLL2 complex recruitment. PMID: 26902498
7. this study demonstrated that SNPs in the coding region of Pygo2 might be one of the causative factors in idiopathic oligospermia and azoospermia, resulting in male infertility. PMID: 26345837
8. Our findings suggest that Pygopus-2 may be an important predictor of poor outcome in HCC patients, and could serve as a novel biomarker for HCC. PMID: 25545771
9. Pygopus-2 over-expression is associated with hepatic carcinoma. PMID: 25871475
10. Pygo2 is a common node downstream of oncogenic Wnt and Akt signaling pathways. PMID: 26170450
11. The results of the present study suggest a novel involvement of Pygo in the promotion of rRNA transcription in cancer cells PMID: 23517060
12. We conclude that abnormal Pygo2 protein expression may be a marker for advanced non-small cell lung cancer PMID: 23865714
13. PYGO2 is identified as a new molecular marker of invasive tumors in esophageal squamous cell carcinoma. PMID: 23456637
14. SNPs in the coding region of Pygo2 gene may be one of the causative factors in idiopathic oligospermia and azoospermia, resulting in male infertility. PMID: 23732668
15. Pygo2 directly occupies the promoters of multiple histone genes and enhances the acetylation of lysine 56 in histone H3. PMID: 22186018
16. Pygo2 is highly expressed in and promotes the growth of glioma cells PMID: 20361361
17. The study demonstrated that Pygo2 was highly expressed in glioma tissue and required for growth of glioblastoma cells. PMID: 20204459
18. Data show that Pygo2 associates with MLL2 histone methyltransferase and STAGA histone acetyltransferase to facilitate their interaction with beta-catenin and Wnt1-induced, TCF/LEF-dependent transactivation in breast cancer cells. PMID: 20937768
19. Pygo2 PHD is the only known PHD finger that is capable of interacting simultaneously with two functional ligands, B9L and BCL9 PMID: 20637214
20. The Expression of pygopus 2 protein mRNA levels were significantly higher in the epithelial ovarian cancer cell lines. PMID: 16609037
21. hPygo2 is highly expressed in, and required for the growth of breast carcinoma cells PMID: 17203217
22. These results provide new evidence that Elf-1 is involved in transcriptional activation of hPygo2. PMID: 18314487
23. Data support a model in which the NHD region of Pygopus is required to augment transcriptional activation by a mechanism that includes both transcriptional activation and histone acetylation resulting from the recruitment of the CBP. PMID: 19555349
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亞細(xì)胞定位：

Nucleus.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 30257

OMIM: 606903

KEGG: hsa:90780

STRING: 9606.ENSP00000357442

UniGene: Hs.533597

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