PYGO2 Antibody

中文名稱：

PYGO2兔多克隆抗體
貨號：

CSB-PA883586LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA883586LA01HU diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA883586LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA883586LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) PYGO2 Polyclonal antibody
Uniprot No.：

Q9BRQ0
基因名：

PYGO2
別名：

1190004M21Rik antibody; FLJ33226 antibody; PP7910 antibody; PYGO2 antibody; Pygo2 protein antibody; PYGO2_HUMAN antibody; Pygopus 2 antibody; pygopus homolog 2 (Drosophila) antibody; Pygopus homolog 2 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Pygopus homolog 2 protein (175-325AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，PYGO2 Antibody (CSB-PA883586LA01HU)，的標記方式是Non-conjugated。對于PYGO2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA883586LB01HU	PYGO2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA883586LC01HU	PYGO2 Antibody, FITC conjugated
Biotin	CSB-PA883586LD01HU	PYGO2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in signal transduction through the Wnt pathway.
基因功能參考文獻：
1. Elevated PYGO2 expression in primary prostate adenocarcinoma is a potential risk factor for biochemical recurrence. PMID: 28924059
2. Overexpression of Pygo2 facilitated the expression of P-glycoprotein, which acts as a drug efflux pump, by promoting the transcription of MDR1 at the MDR1 promoter loci, resulting in acceleration of the efflux of paclitaxel in human glioma cells. PMID: 28427190
3. We also determined the effect of Pygo2 on the sensitivity of breast tumors resistant to doxorubicin in a mouse model. Finally, RNA samples from 64 paired patient tumors (before and after chemotherapy) highly and significantly overexpressed Pygo2 and/or MDR1 after treatment, thus underlining a pivotal role for the Pygo2-mediated Wnt/b-catenin pathway in the clinical chemoresistance of breast cancer. PMID: 26876203
4. Findings are consistent with a model in which acetylation of Pygo2 by CBP/p300 family members in the active TCF/beta-catenin complex occurs coincident with histone acetylation and may be required for the recycling of Pygo2 away from the complex subsequent to target gene activation. PMID: 27647933
5. The activation of its expression by ERalpha and/or specificity protein-1 (SP1) suggests hPYGO2 as a theranostic target for hormone therapy responsive and refractory breast cancer. PMID: 26645832
6. Pygo2 functions as a prognostic factor for glioma due to its up-regulation of H3K4me3 and promotion of MLL1/MLL2 complex recruitment. PMID: 26902498
7. this study demonstrated that SNPs in the coding region of Pygo2 might be one of the causative factors in idiopathic oligospermia and azoospermia, resulting in male infertility. PMID: 26345837
8. Our findings suggest that Pygopus-2 may be an important predictor of poor outcome in HCC patients, and could serve as a novel biomarker for HCC. PMID: 25545771
9. Pygopus-2 over-expression is associated with hepatic carcinoma. PMID: 25871475
10. Pygo2 is a common node downstream of oncogenic Wnt and Akt signaling pathways. PMID: 26170450
11. The results of the present study suggest a novel involvement of Pygo in the promotion of rRNA transcription in cancer cells PMID: 23517060
12. We conclude that abnormal Pygo2 protein expression may be a marker for advanced non-small cell lung cancer PMID: 23865714
13. PYGO2 is identified as a new molecular marker of invasive tumors in esophageal squamous cell carcinoma. PMID: 23456637
14. SNPs in the coding region of Pygo2 gene may be one of the causative factors in idiopathic oligospermia and azoospermia, resulting in male infertility. PMID: 23732668
15. Pygo2 directly occupies the promoters of multiple histone genes and enhances the acetylation of lysine 56 in histone H3. PMID: 22186018
16. Pygo2 is highly expressed in and promotes the growth of glioma cells PMID: 20361361
17. The study demonstrated that Pygo2 was highly expressed in glioma tissue and required for growth of glioblastoma cells. PMID: 20204459
18. Data show that Pygo2 associates with MLL2 histone methyltransferase and STAGA histone acetyltransferase to facilitate their interaction with beta-catenin and Wnt1-induced, TCF/LEF-dependent transactivation in breast cancer cells. PMID: 20937768
19. Pygo2 PHD is the only known PHD finger that is capable of interacting simultaneously with two functional ligands, B9L and BCL9 PMID: 20637214
20. The Expression of pygopus 2 protein mRNA levels were significantly higher in the epithelial ovarian cancer cell lines. PMID: 16609037
21. hPygo2 is highly expressed in, and required for the growth of breast carcinoma cells PMID: 17203217
22. These results provide new evidence that Elf-1 is involved in transcriptional activation of hPygo2. PMID: 18314487
23. Data support a model in which the NHD region of Pygopus is required to augment transcriptional activation by a mechanism that includes both transcriptional activation and histone acetylation resulting from the recruitment of the CBP. PMID: 19555349
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亞細胞定位：

Nucleus.
數據庫鏈接：

HGNC: 30257

OMIM: 606903

KEGG: hsa:90780

STRING: 9606.ENSP00000357442

UniGene: Hs.533597

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