LEF1 Recombinant Monoclonal Antibody

說明書

中文名稱：

LEF1重組抗體
貨號：

CSB-RA561630A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA561630A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.

IHC image of CSB-RA561630A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA561630A0HU LEF1重組單克隆抗體是通過哺乳動物表達系統制備的高特異性科研試劑，靶向Wnt/β-catenin信號通路關鍵調控因子淋巴增強因子1（LEF1）。該抗體經嚴格驗證可在1:50-1:200稀釋范圍內實現穩定可靠的免疫組化（IHC）檢測，同時在ELISA平臺展現優異的抗原結合能力，其高親和力特性確保對目標蛋白的特異性識別，實驗重復性良好，染色定位精準且背景信號低。LEF1作為調控胚胎發育及細胞分化的核心轉錄因子，在腫瘤發生、干細胞自我更新及組織再生等研究中具有重要價值，本產品適用于探究腫瘤微環境中Wnt信號通路的異常激活機制、干細胞分化模型中的分子標記分析，以及發育生物學相關蛋白的時空表達定位研究。重組單抗技術保障了批次間的高度一致性，為體外實驗及病理樣本的蛋白水平檢測提供標準化工具。
Uniprot No.：

Q9UJU2
基因名：

LEF1
別名：

Lymphoid enhancer-binding factor 1 (LEF-1) (T cell-specific transcription factor 1-alpha) (TCF1-alpha), LEF1
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human LEF1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

13B7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicle morphogenesis.; Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.; Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.; Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
基因功能參考文獻：
1. Experimental analysis showed the importance of LEF1, ETV4 and FABP6 as three co-regulated prognostic markers in patients with colorectal cancer metastasis. PMID: 30193961
2. LEF-1 and CDX2 performed at least as well as beta-catenin, if not better, as a diagnostic marker for pilomatrical carcinomas PMID: 29369390
3. study identifies a novel mechanism of hepatocellular carcinoma inhibition through beta-catenin-independent Wnt signalling, which is regulated by WT1-associated LEF1 repression. The study also highlights mangiferin as a promising Wnt inhibitor for HCC treatment. PMID: 29953980
4. High LEF1 expression is associated with papillary thyroid carcinoma. PMID: 28677753
5. LEF1 was consistently expressed in the tubal-peritoneal junctions and all tubal intraepithelial lesions, independent of p53 status. PMID: 28664938
6. these data suggest that LEF1 rather has tumor suppressive functions and attenuates aggressiveness in a subset of RMS. PMID: 27965462
7. Positive LEF-1 favors a benign neoplasm. PMID: 28972308
8. Results show that high enhancer-binding factor-1 (LEF1) expression is associated with poor survival for chronic lymphocytic leukemia (CLL) patients. PMID: 26950276
9. Knockdown of GATA6 completely eliminated the effect of TCF1, while forced expression of GATA6 induced hESC differentiation PMID: 28943339
10. Lef1 and Tcf1 showed oncogenic effect in colonic carcinogenesis. Cellular context of miRNAs might play important roles in carcinogenesis by altering the expression pattern of Lef/Tcfs members. PMID: 27433921
11. Data indicate that microRNA miR-27a directly targets GSK-3beta and increases expression of beta-catenin and LEF1 in all-trans-retinoic acid (ATRA)-induced Hep2 cells. PMID: 28122350
12. and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
13. MicroRNA-557 might work as a tumor suppressor by negatively regulating the expression of lymphocyte enhancement factor 1 in lung cancer cells. PMID: 28639890
14. EVI1 transcription is directly regulated by LEF1/beta-catenin complex in myeloid blast crisis of chronic myeloid leukemia. Loss of p53 function as a key regulator for beta-catenin-EVI1 in myeloid blast crisis of chronic myeloid leukemia. PMID: 27908728
15. LEF1 is a sensitive and specific marker for chronic lymphocytic B-cell leukemia PMID: 28395058
16. a frequency of 4-9% expression of LEF1 bymantle cell lymphoma PMID: 28038713
17. The results suggest a role for beta-catenin/LEF1-mediated transcription in both malignant transformation and metastasis of prostate cancer PMID: 27067790
18. The results describe a novel mutation in LEF1 causing dysregulation of Wnt/B catenin signaling in eyelid sebaceous cancinoma PMID: 25639559
19. LEF-1 and MITF regulate tyrosinase gene transcription in vitro via binding to its promoter. PMID: 26580798
20. Methylprednisolone can suppress Wnt signaling pathway by down-regulating LEF-1 protein expression in chronic lymphocyte leukemia cells. PMID: 26339357
21. Overexpression of LEF1 is a favorable prognostic factor in childhood ALL. PMID: 25955539
22. Authors found that the N-terminus of delta-catenin bound to the middle region of LEF-1 unlike beta-catenin. Overexpressed delta-catenin entered the nucleus and inhibited LEF-1-mediated transcriptional activity in Bosc23 and DLD-1 cell lines. PMID: 25808920
23. high LEF1 expression and mutation are associated with high-risk leukemia and our results also revealed that LEF1 high expression and/or gain-of-function mutations are involved in leukemogenesis of ALL. PMID: 25942645
24. Study found a negative correlation between miR-34a and LEF1 expression in prostate cancer cell lines and tumors and that miR-34a regulated epithelial-mesenchymal transition through direct binding to LEF1 mRNA 3' UTR region and silencing its translation. PMID: 25587085
25. Our study suggests LEF1 expression in oral squamous cell carcinoma may play an important role in tumor progression and can be served as a predictor of poor prognosis for patients with oral squamous cell carcinoma. PMID: 24021930
26. Elevated TCF-1 and LEF-1 expression is characteristic of malignant gliomas PMID: 24858819
27. LEF-1 expression is associated with the presence of KRAS mutations and may have prognostic value as a trend of worse overall survival is seen in patients with LEF-1-positive colorectal carcinoma. PMID: 25394300
28. These results further implicate the CTNNB1/LEF1 transcriptional complex in the development of solid-pseudopapillary neoplasms of the pancreas PMID: 24658583
29. study identified TCF1 and LEF1 as Tax antagonistic factors in vivo, a fact which may critically influence the peripheral T-cell tropism of this virus PMID: 25646419
30. miR26b may act as a potential therapeutic agent in reducing cancer cell proliferation through repressing LEF1 activation of c-Myc and cyclin D1 expression PMID: 24785257
31. Tcf1 and Lef1 cooperate with Runx factors to achieve stable silencing of the Cd4 gene in CD8(+) T cells. PMID: 24847765
32. LEF-1 expression in both basal cell adenomas and basal cell adenocarcinomas preferentially over other salivary gland tumors suggesting some utility as a diagnostic marker PMID: 25497834
33. Proper sample processing ensured sufficient separation of positive LEF1 staining in T cells from negative staining in normal B and natural killer (NK) cells PMID: 25596247
34. LEF1 expression is an independent prognostic factor in APL, and could be used in patients risk stratification. PMID: 24378360
35. Selenite caused CYLD upregulation via LEF1 and cIAP downregulation, both of which contribute to the degradation of ubiquitin chains on RIP1 and subsequent caspase-8 activation and colorectal tumor cell apoptosis. PMID: 24577083
36. Results indicate that lymphoid enhancer-binding factor 1 (LEF1) contributes to the pathophysiology of acute myeloid leukemia (AML) and could be a predictor of better treatment response. PMID: 23713453
37. the expression of LEF1 is associated with the progression of human renal cell carcinoma and LEF1 maybe involved in the development of RCC PMID: 24897388
38. By beta-catenin's association with LEF1 and BCL9-2/B9L. PMID: 24419084
39. LEF1 protein was overexpressed in colon cancer tissues and knockdown of LEF1 expression inhibited colon cancer growth in vitro and in vivo. PMID: 24098538
40. Studied the expression of TCF/LEF and SFRP family members (SFRP1 and SFRP3) to gain a better understanding of biological signaling pathways responsible for epidemiology and clinical parameters of clear cell RCC (cRCC). PMID: 23572277
41. High LEF1 expression is associated with prostate cancer. PMID: 23913826
42. DNA-dependent protein kinase catalytic subunit is a novel interaction partner of lymphocyte enhancer factor 1. PMID: 23325550
43. This study supports a functionally important role for LEF1 and its target genes in Burkitt's lymphoma. PMID: 23375451
44. this study demonstrated that miR-449a directly targets LEF-1, which in turn affects the expression of Sox 9, ultimately leading to the proper regulation of the differentiation and chondrogenesis PMID: 22769578
45. Nuclear LEF1 correlate with cerebral metastasis of lung adenocarcinomas. PMID: 23224985
46. LEF-1 might play an important role in colon carcinogenesis by acting as a regulator. PMID: 22639890
47. Lef1 is overexpressed in human endometrial tumors, consistent with it playing a role in gland proliferation. PMID: 22792274
48. An association between the maternal genotype and the occurrence of cleft lip or palate was observed at two polymorphic loci (rs10022956 and rs10025431), while a foetal-maternal effect modulating the risk of clefting was found at locus rs10025431. PMID: 21781440
49. n-Butyl benzyl phthalate promotes breast cancer progression by inducing expression of lymphoid enhancer factor 1 PMID: 22905168
50. interaction of HCLS1 with LEF-1 is essential for G-CSF-triggered myeloid differentiation PMID: 23001182
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亞細胞定位：

Nucleus.
蛋白家族：

TCF/LEF family
組織特異性：

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several
數據庫鏈接：

HGNC: 6551

OMIM: 153245

KEGG: hsa:51176

STRING: 9606.ENSP00000265165

UniGene: Hs.743478

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