Flow cytometric analysis of keratin 4 expression in H9C2 cells using keratin 4 antibody. Green, isotype control; red, keratin 4.
Immunocytochemical staining of H9c2 cells with Keratin 4 antibody. Nuclei were stained blue with DAPI; Keratin 4 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using keratin 4 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed wide