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中文名稱：TMEM176B兔多克隆抗體
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貨號：CSB-PA023758LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: THP-1 whole cell lysate(30μg), SY5Y whole cell lysate(30μg), Hela whole cell lysate(30μg), K562 whole cell lysate(30μg)
All lanes: TMEM176B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 30,26 kDa
Observed band size: 30 kDa
Exposure time：120s -
Immunofluorescence staining of Hela cell with CSB-PA023758LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：
To produce the TMEM176B antibody, a rabbit was first immunized with the recombinant human transmembrane protein 176B (1-64aa). Following this, the rabbit serum was collected and the antibody was isolated and purified through protein G affinity chromatography.
The resulting antibody showed high purity levels of up to 95% and displayed reactivity solely with human TMEM176B protein. Quality verification tests were conducted on the rabbit anti-human TMEM176B polyclonal antibody, including ELISA, WB, IHC, and IF, confirming its suitability for use in various applications.
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) TMEM176B Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：TMEM176B; LR8; Transmembrane protein 176B; Protein LR8
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宿主：Rabbit
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反應(yīng)種屬：Human
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免疫原：Synthesized peptide derived from human TMEM176B
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免疫原種屬：Homo sapiens (Human)
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen affinity purification
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:10-1:100 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻
- Single-cell mRNA-regulation analysis reveals cell type-specific mechanisms of type 2 diabetes J Martinez-Lopez,bioRxiv,2023
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：May play a role in the process of maturation of dendritic cells. Required for the development of cerebellar granule cells.
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基因功能參考文獻：
- Study characterized Tmem176a and b expression in RORgamma t+ lymphocytes at transcriptional and protein levels and present evidence that both genes exert a redundant ion channel function related to a colocalisation in close proximity to the Golgi apparatus. PMID: 27009467
- human TMEM176A and 176B protein levels are significantly elevated in lymphoma, but not in normal tissues PMID: 22244448
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亞細胞定位：Nucleus membrane; Multi-pass membrane protein.
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蛋白家族：TMEM176 family
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組織特異性：Expressed in lung and dermal fibroblasts.
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數(shù)據(jù)庫鏈接：
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