Rat Complement fragment 3a,C3a ELISA Kit

Rat Complement fragment 3a,C3a ELISA Kit

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中文名稱：

大鼠補體片斷3a(C3a)酶聯免疫試劑盒
貨號：

CSB-E08510r
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

大鼠補體片斷3a(C3a)酶聯免疫試劑盒（CSB-E08510r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3a含量。C3a是補體系統的重要成分，由C3蛋白裂解產生，參與免疫和炎癥反應。其研究機制涉及C3a與細胞表面受體的相互作用，激活下游信號通路，調節免疫細胞功能。C3a在多種疾病中發揮作用，如糖尿病腎病和多發性骨髓瘤骨病，成為潛在的治療靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，本試劑盒檢測靈敏度覆蓋1.56 ng/mL至100 ng/mL寬動態范圍，可穩定識別樣本中痕量目標蛋白。通過優化的預包被抗體與酶標記檢測抗體協同作用，確保檢測結果的高特異性和重復性，適用于科研場景中炎癥機制研究、免疫相關疾病動物模型評估或藥物干預實驗中C3a動態變化的監測，為探索補體系統在生理病理中的作用提供可靠工具。實驗操作簡便，兼容常規實驗室設備，配套標準品及檢測緩沖液，支持快速完成樣本檢測與數據分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

C3a
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	88
	Range %	80-97
1:10	Average %	95
	Range %	92-102
1:20	Average %	96
	Range %	90-102
1:40	Average %	93
	Range %	90-96

回收率：

The recovery of rat C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	101	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.588	2.598	2.593	2.411
50	1.948	1.903	1.926	1.744
25	1.431	1.468	1.450	1.268
12.5	1.009	1.051	1.030	0.848
6.25	0.624	0.637	0.631	0.449
3.12	0.414	0.438	0.426	0.244
1.56	0.279	0.291	0.285	0.103
0	0.181	0.183	0.182

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Potassium/Sodium Citrate Attenuates Paclitaxel-Induced Peripheral Neuropathy D Uta, H Nakamura, K Maruo, K Matsumura,International Journal of Molecular Sciences,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.