Mouse Complement fragment 3a,C3a ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠補體片斷3a(C3a)酶聯免疫試劑盒（CSB-E08511m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的C3a含量。C3a是重要的補體激活產物。在生理情況下參與免疫調節、組織修復等。其研究機制主要聚焦于免疫反應調控，它能通過與受體結合，影響免疫細胞功能，在炎癥、自身免疫病等病理過程中發揮關鍵作用，是潛在治療靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產品適用于基礎免疫學研究中補體系統激活機制的探索、炎癥性疾病動物模型的生物標志物分析，以及藥物研發中抗炎或免疫調節劑的效果評估。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

C3a
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	93
	Range %	89-100
1:20	Average %	96
	Range %	91-101
1:40	Average %	99
	Range %	93-105
1:80	Average %	88
	Range %	82-95

回收率：

The recovery of mouse C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-104
EDTA plasma (n=4)	93	88-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.