• A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human C3a antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
• Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
• One vial Biotin-labeled C3a antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
• One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
• One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
• One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
• One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
• One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
• One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
• One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
• An instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

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