Rabbit Procalcitonin (PCT) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔降鈣素原(PCT)酶聯(lián)免疫試劑盒（CSB-E12875Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的PCT含量。PCT即降鈣素原，在健康人群中含量極低。當(dāng)機(jī)體發(fā)生細(xì)菌感染時(shí)，PCT會(huì)明顯升高。其研究機(jī)制主要是通過(guò)檢測(cè)它在血液中的水平，輔助醫(yī)生判斷是否存在細(xì)菌感染、評(píng)估感染嚴(yán)重程度及指導(dǎo)抗生素的合理使用。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，常用于科研領(lǐng)域評(píng)估炎癥程度及疾病進(jìn)展；可為感染性疾病動(dòng)物模型研究、免疫機(jī)制探索及藥物療效評(píng)價(jià)提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

PCT
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-102
1:2	Average %	86
	Range %	81-99
1:4	Average %	95
	Range %	90-103
1:8	Average %	93
	Range %	84-100

回收率：

The recovery of rabbit PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	86-94
EDTA plasma (n=4)	96	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.