Canine Procalcitonin(PCT)ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

犬降鈣素原(PCT)酶聯(lián)免疫試劑盒（CSB-E17400c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的PCT含量。PCT即降鈣素原，在健康人體中含量極低。細菌感染尤其是嚴重的全身感染時，PCT水平會顯著升高。其研究機制是細菌內(nèi)毒素等可刺激多種細胞產(chǎn)生PCT，臨床上常通過檢測其數(shù)值輔助判斷是否存在細菌感染、評估感染嚴重程度及預后。試劑盒檢測范圍為46.88 pg/mL-3000 pg/mL，在科研領(lǐng)域，該產(chǎn)品可用于探索犬類感染性疾病模型的病理機制、評估抗生素治療過程中炎癥反應(yīng)動態(tài)變化，或作為免疫調(diào)節(jié)藥物開發(fā)的生物標志物檢測工具，為獸醫(yī)基礎(chǔ)研究及實驗動物科學提供可靠的定量分析解決方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PCT
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

46.88 pg/mL-3000 pg/mL
靈敏度：

11.72 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-90
1:2	Average %	95
	Range %	91-99
1:4	Average %	91
	Range %	85-97
1:8	Average %	83
	Range %	80-86

回收率：

The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-93
EDTA plasma (n=4)	104	101-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.