Rat procalcitonin (PCT) ELISA kit

產品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠降鈣素原(PCT)酶聯免疫試劑盒（CSB-E13419r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PCT含量。PCT即降鈣素原，在細菌感染等引發的全身炎癥反應中會顯著升高。作為一種新型炎癥標志物。研究機制主要是在感染時，細菌內毒素及炎性因子刺激其大量合成，可用于鑒別細菌與病毒感染、評估感染嚴重程度等。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外實驗中對不同來源樣本的PCT水平檢測。研究者可借助探索炎癥相關疾病的分子機制、細菌感染模型的動態變化，或評估抗炎藥物、免疫調節劑的干預效果。可為感染免疫學、藥理學等領域的實驗提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PCT
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	96
	Range %	91-103
1:4	Average %	97
	Range %	91-105
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of rat PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-107
EDTA plasma (n=4)	97	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.