Mouse Homocysteine(HCY)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠高半胱氨酸(Hcy)酶聯(lián)免疫試劑盒（CSB-E16551m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代謝過(guò)程中的中間產(chǎn)物。其水平異常升高是心血管疾病等多種疾病的獨(dú)立危險(xiǎn)因素。研究機(jī)制方面，高Hcy可損傷血管內(nèi)皮細(xì)胞，影響脂質(zhì)代謝，還能促進(jìn)血栓形成，干擾一氧化氮合成，從而引發(fā)一系列病理變化。試劑盒檢測(cè)范圍為4.7 nmol/mL-300 nmol/mL，適用于小鼠模型中的代謝功能評(píng)估、疾病進(jìn)程監(jiān)測(cè)或藥物干預(yù)效果分析，例如在動(dòng)脈粥樣硬化、阿爾茨海默病等研究中動(dòng)態(tài)觀察Hcy水平變化，或在營(yíng)養(yǎng)干預(yù)實(shí)驗(yàn)中探究維生素B族對(duì)Hcy代謝的調(diào)控作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

N/A
縮寫：

Hcy
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

4.7 nmol/mL-300 nmol/mL
靈敏度：

1.1 nmol/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	90
	Range %	86-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	99
	Range %	95-107

回收率：

The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.