Rat Homocysteic acid,Hcy ELISA Kit

產(chǎn)品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠同型半胱氨酸(Hcy)酶聯(lián)免疫試劑盒（CSB-E08896r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代謝過程中的中間產(chǎn)物。其水平升高被視為心腦血管疾病等多種病癥的獨立危險因素。研究機制主要集中在其對血管內(nèi)皮細(xì)胞的損傷、促進血栓形成及氧化應(yīng)激反應(yīng)等方面，深入研究它有助于疾病防治。試劑盒檢測范圍為0.78 nmol/mL-50 nmol/mL，適用于科研場景下對大鼠模型體內(nèi)Hcy代謝水平的動態(tài)監(jiān)測，例如營養(yǎng)代謝研究、藥物毒性評價或疾病機制探索。可廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)、藥理學(xué)及動物模型構(gòu)建等領(lǐng)域，助力Hcy相關(guān)分子機制的深入解析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

Hcy
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 nmol/mL-50 nmol/mL
靈敏度：

0.195 nmol/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-108
1:2	Average %	96
	Range %	92-100
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	97	94-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.