Bovine secretory immunoglobulin A,sIgA ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛分泌型免疫球蛋白A(sIgA)酶聯免疫試劑盒（CSB-E11192b）為競爭法ELISA試劑盒，定量檢測唾液樣本中的sIgA含量。sIgA即分泌型免疫球蛋白 A，是黏膜免疫系統的關鍵效應分子，在抵御病原體入侵方面發揮重要作用。其能阻止病原體黏附于上皮細胞，中和毒素。研究機制著重于其產生調控、與微生物相互作用及在疾病發生發展中的變化。試劑盒檢測范圍為2.4 μg/mL-150 μg/mL，適用于科研領域中對牛黏膜免疫功能的評估，如病原體感染機制研究、疫苗免疫效果監測、飼料添加劑對黏膜免疫的影響分析等場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

sIgA
種屬：

Bos taurus (Bovine)
樣本類型：

saliva
檢測范圍：

2.4 μg/mL-150 μg/mL
靈敏度：

2.4 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	92
	Range %	85-101
1:40	Average %	95
	Range %	90-99
1:80	Average %	93
	Range %	85-97
1:160	Average %	97
	Range %	92-110

回收率：

The recovery of bovine sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-100
EDTA plasma (n=4)	94	87-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.