Mouse secretory immunoglobulin A,sIgA ELISA Kit

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中文名稱：

小鼠分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08413m
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒（CSB-E08413m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、支氣管肺泡灌洗液樣本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫系統(tǒng)的主要效應(yīng)分子。能夠在黏膜表面阻止病原體黏附與入侵，發(fā)揮抗感染等作用。研究機(jī)制主要圍繞其生成、分泌過(guò)程，以及與病原體相互作用等方面，以探索其在疾病防治中的應(yīng)用潛力。試劑盒檢測(cè)范圍為15.6 ng/mL-1000 ng/mL，適用于科研場(chǎng)景中黏膜免疫相關(guān)動(dòng)物模型的機(jī)制研究，例如呼吸道感染模型中局部免疫應(yīng)答的動(dòng)態(tài)監(jiān)測(cè)、腸道炎癥疾病中黏膜屏障功能的評(píng)估，或藥物/益生菌干預(yù)后sIgA分泌水平的定量分析。其寬泛的樣本兼容性可支持多維度實(shí)驗(yàn)設(shè)計(jì)，尤其適用于支氣管肺泡灌洗液等低濃度樣本的檢測(cè)需求，為黏膜免疫調(diào)控機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫：

sIgA
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, bronchoalveolar lavage fluid
檢測(cè)范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	92
	Range %	87-95
1:40	Average %	101
	Range %	97-104
1:80	Average %	93
	Range %	87-97
1:160	Average %	98
	Range %	92-102

回收率：

The recovery of mouse sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	92-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.486	2.466	2.476	2.312
500	1.772	1.841	1.807	1.643
250	1.232	1.222	1.227	1.063
125	0.760	0.748	0.754	0.590
62.5	0.464	0.481	0.473	0.309
31.2	0.325	0.312	0.319	0.155
15.6	0.239	0.229	0.234	0.070
0	0.166	0.162	0.164