Rat secretory immunoglobulin A,sIgA ELISA Kit

Rat secretory immunoglobulin A,sIgA ELISA Kit

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中文名稱：

大鼠分泌型免疫球蛋白A(sIgA)酶聯免疫試劑盒
貨號：

CSB-E08412r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠分泌型免疫球蛋白A(sIgA)酶聯免疫試劑盒（CSB-E08412r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫的重要效應分子。它可阻止病原體黏附上皮細胞、中和毒素及病毒等。研究主要聚焦其產生機制、在黏膜免疫防御中的作用機制等，對揭示機體抗感染免疫及開發相關疫苗等有重要意義。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，在科研領域，本試劑盒可用于研究大鼠模型中黏膜免疫相關疾病（如腸道炎癥、呼吸道感染）的發病機制，評估免疫調節劑或益生菌干預效果，亦可應用于營養學、毒理學研究中黏膜屏障功能的動態監測。其多樣本類型兼容性為實驗設計提供靈活性，例如通過組織勻漿檢測局部黏膜sIgA表達，或通過體液樣本評估系統免疫狀態。本產品嚴格遵循質量控制標準，提供標準品及預包被板等完整實驗組件，確保實驗數據的穩定性和可重復性，為科研人員探索黏膜免疫調控網絡提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

sIgA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	97-106
1:200	Average %	90
	Range %	86-96
1:400	Average %	97
	Range %	92-103
1:800	Average %	88
	Range %	84-92

回收率：

The recovery of rat sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.511	2.371	2.441	2.302
25	2.046	1.976	2.011	1.872
12.5	1.512	1.501	1.507	1.368
6.25	0.979	1.003	0.991	0.852
3.12	0.482	0.518	0.500	0.361
1.56	0.334	0.359	0.347	0.208
0.78	0.241	0.229	0.235	0.096
0	0.143	0.135	0.139

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Lycopene Modulates Placental Health and Fetal Development Under High‐Fat Diet During Pregnancy of Rats S Sun,Molecular Nutrition & Food Research,2021
Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats Jian Zhou.et al,British Journal of Nutrition,2018
Modulating Effect of Peptide Therapy on the Morphofunctional State of Bronchial Epithelium in Rats with Obstructive Lung Pathology. Kuzubova NA.et al,Bull Exp Biol Med.,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.