Bovine procalcitonin (PCT) ELISA kit

產品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛降鈣素原(PCT)酶聯免疫試劑盒（CSB-E15004B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PCT含量。降鈣素原（PCT）是一種蛋白質。在健康人血液中含量極低，而在嚴重細菌、真菌、寄生蟲感染及膿毒癥和多臟器功能衰竭時，其水平會異常升高。它可作為炎癥和感染的生物標志物，研究其機制有助于深入了解感染進程并指導治療。試劑盒檢測范圍為39.06 pg/mL-2500 pg/mL，可為獸醫病理研究、畜牧動物健康監測及牛源性疾病模型建立提供關鍵生物標志物檢測支持，特別適用于評估反芻動物炎癥反應進程、疫苗效力測試及抗生素治療研究等基礎科研領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PCT
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue Homogenates
檢測范圍：

39.06 pg/mL-2500 pg/mL
靈敏度：

9.77 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-97
1:2	Average %	93
	Range %	90-97
1:4	Average %	95
	Range %	96-97
1:8	Average %	95
	Range %	88-98

回收率：

The recovery of bovine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-97
EDTA plasma (n=4)	92	90-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.