Recombinant Mouse Metal cation symporter ZIP14 (Slc39a14)

中文名稱：

Recombinant Mouse Metal cation symporter ZIP14 (Slc39a14)
貨號：

CSB-CF748446MO
說明書：

MSDS Datasheet
規格：

￥9720
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Slc39a14
Uniprot No.：

Q75N73
別名：

Factor for adipocyte differentiation 123;FAD-123;Solute carrier family 39 member 14;Zrt- and Irt-like protein 14;ZIP-14
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

in vitro E.coli expression system
分子量：

52.3 kDa
表達區域：

29-489aa
氨基酸序列

SSAGLPPLSATSFLEDLMDRYGKNDSLTLTQLKSLLDHLHVGVGRDNVSQPKEGPRNLSTCFSSGDLFAAHNLSERSQIGASEFQEFCPTILQQLDSQACTSENQKSEENEQTEEGKPSAIEVWGYGFLCVTVISLCSLMGASVVPFMKKTFYKRLLLYFIALAIGTLYSNALFQLIPEAFGFNPQDNYVSKSAVVFGGFYLFFFTEKILKMLLKQKNEHHHGHNHFTSETLPSKKDQEEGVTEKLQNGDLDHMIPQHCNSELDGKAPGTDEKVIVNSMSVQDLQASQSACYWLKGVRYSDIGTLAWMITLSDGLHNFIDGLAIGASFTVSVFQGISTSVAILCEEFPHELGDFVILLNAGMSIQQALFFNFLSACCCYLGLAFGILAGSHFSANWIFALAGGMFLYIALADMFPEMNEVCQEDEKNDSFLVPFVIQNLGLLTGFSIMLVLTMYSGQIQIG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Electroneutral transporter of the plasma membrane mediating the cellular uptake of the divalent metal cations zinc, manganese and iron that are important for tissue homeostasis, metabolism, development and immunity. Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation and two bicarbonate anions. Beside these endogenous cellular substrates, can also import cadmium a non-essential metal which is cytotoxic and carcinogenic. Controls the cellular uptake by the intestinal epithelium of systemic zinc, which is in turn required to maintain tight junctions and the intestinal permeability. Modifies the activity of zinc-dependent phosphodiesterases, thereby indirectly regulating G protein-coupled receptor signaling pathways important for gluconeogenesis and chondrocyte differentiation. Regulates insulin receptor signaling, glucose uptake, glycogen synthesis and gluconeogenesis in hepatocytes through the zinc-dependent intracellular catabolism of insulin. Through zinc cellular uptake also plays a role in the adaptation of cells to endoplasmic reticulum stress. Major manganese transporter of the basolateral membrane of intestinal epithelial cells, it plays a central role in manganese systemic homeostasis through intestinal manganese uptake. Also involved in manganese extracellular uptake by cells of the blood-brain barrier. May also play a role in manganese and zinc homeostasis participating in their elimination from the blood through the hepatobiliary excretion. Also functions in the extracellular uptake of free iron. May also function intracellularly and mediate the transport from endosomes to cytosol of iron endocytosed by transferrin. Plays a role in innate immunity by regulating the expression of cytokines by activated macrophages.
基因功能參考文獻：
1. This study concludes that SLC39A14 is essential for efficient Mn uptake by the liver and pancreas, and its deficiency results in impaired Mn excretion and accumulation of the metal in other tissues. PMID: 29437953
2. Data indicate SLC39A14 (ZIP14) as a regulator of bone homeostasis, and that manipulating ZIP14 might be a therapeutic strategy for bone diseases. PMID: 29621230
3. Zip14 activity is needed for adaptation to endoplasmic reticulum stress in liver. PMID: 28673968
4. Data suggest that most if not all tissues use ZIP8 and ZIP14A/ZIP14B (alternative splicing products) for Zn(2+) uptake, some tissues under basal conditions and others more so when inflammatory stressors are present (as in endotoxemia); collectively, this might lead to substantial alterations in plasma Zn(2+) levels due to Zn(2+) redistribution not just in liver but across many vital organs. PMID: 24728862
5. the inflammation-responsive zinc transporter ZIP14 has phenotypic effects that are amplified with aging PMID: 27647172
6. ZIP14 is essential for manganese elimination via the gastrointestinal tract, and a lack of ZIP14 results in manganese accumulation in critical tissues such as the brain PMID: 28536273
7. Intravitreal holo-transferrin injection decreased Zip 14 protein levels. These data indicate that Zip8 and Zip14 may take up increasing amounts of non-transferrin bound iron in these two mouse models of retinal iron accumulation. PMID: 28057442
8. ZIP14-mediated zinc transport contributes to regulation of endosomal insulin receptor activity and glucose homeostasis in hepatocytes. PMID: 27703010
9. Results suggest that aberrant zinc distribution observed with Zip14 ablation impacts adipose cytokine production and metabolism, ultimately increasing fat deposition when exposed to endotoxin. PMID: 26646099
10. Slc39a14 deficiency in hemochromatotic mice greatly diminished iron loading of the liver and prevented iron deposition in hepatocytes and pancreatic acinar cells. PMID: 26028554
11. PrP(C) promotes, and possibly regulates, the uptake of iron through DMT1 and Zip14 via its ferrireductase activity. PMID: 25862412
12. Zinc transporter ZIP14 influences aspects of the pathophysiology of nonlethal polymicrobial murine sepsis induced by cecal ligation/puncture through zinc delivery. PMID: 26272258
13. Oral cadmium chloride administration to ZIP14 knockout mice results in time dependent changes in gene expression. PMID: 25294218
14. ZIP14 is a basolaterally localized protein in enterocytes and is involved in endosomal trafficking of zinc and is necessary for proper maintenance of intestinal tight junctions PMID: 25428902
15. Hepatic ZIP14 protein levels are upregulated in iron-loaded hypotransferrinemic mice. PMID: 23349308
16. We ligated mouse ZIP14A, ZIP14B and ZIP8 cDNA coding regions into the Xenopus-specific vector pXFRM, transcribed these in vitro, and microinjected the capped RNAs into Xenopus oocytes. K(m) and V(max) values for Cd, Zn and Fe uptake were determined. PMID: 23090441
17. Zip14 expression induced by lipopolysaccharides in macrophages attenuates inflammatory response PMID: 23052185
18. The knockdown of ZIP8, ZIP14 or DMT1 by siRNA transfection significantly reduced the uptake of Cd(2+) and Mn(2+) from the apical membrane. PMID: 22534978
19. This study found that Zip14 is capable of mediating cellular uptake of nontransferrin-bound iron characteristic of iron-overload conditions. PMID: 21653899
20. data highlight SLC39A14 as an important novel player in GPCR-mediated signaling PMID: 21445361
21. These results suggest that endosomal ZIP14 participates in the cellular assimilation of iron from transferrin, thus identifying a potentially new role for ZIP14 in iron metabolism. PMID: 20682781
22. SLC39A14 plays a role as a zinc transporter during the early stages of adipogenesis PMID: 15794747
23. Zip14 expression is up-regulated through IL-6 PMID: 15863613
24. Zip14 can mediate the uptake of zinc and non-transferrin-bound iron into cells. PMID: 16950869
25. The present study provides the complete characterization of Slc39a14 gene and its ZIP14 mRNAs and proteins and for the first time demonstrates the significance of ZIP14 in Cd2+-mediated cell damage. PMID: 18270315
26. These results suggest that signaling pathways activated by nitric oxide are factors in the upregulation of Zip14, which in turn mediates hepatic zinc accumulation and hypozincemia during inflammation and sepsis. PMID: 19179618
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
蛋白家族：

ZIP transporter (TC 2.A.5) family
組織特異性：

Widely expressed. Highly and transiently expressed during the early stage of adipocyte differentiation. Strongly expressed in liver, preadipocyte, duodenum and jejunum, moderately in brain, heart, skeletal muscle, spleen, pancreas, kidney and white adipos
數據庫鏈接：

KEGG: mmu:213053

STRING: 10090.ENSMUSP00000022688

UniGene: Mm.270647