Recombinant Rat Microtubule-associated protein tau (Mapt) (Active)

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中文名稱：

大鼠Mapt重組蛋白
品名簡稱：

Recombinant Rat Mapt protein (Active)
貨號：

CSB-MP013481RA
說明書：

MSDS Datasheet
規格：

￥828
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat Mapt at 2 μg/ml can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU), the EC₅₀ is 136.2-238.9 ng/mL. Biological Activity Assay
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產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Rat Mapt at 2 μg/ml can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU), the EC₅₀ is 136.2-238.9 ng/mL.
基因名：

Mapt
Uniprot No.：

P19332
別名：

(Neurofibrillary tangle protein)(Paired helical filament-tau)(PHF-tau)
分子結構：
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
來源：

Mammalian cell
分子量：

81.2 kDa
表達區域：

2-752aa
氨基酸序列

AEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

This Rat Mapt recombinant protein was produced in mammalian cell, where the gene sequence encoding rat Mapt (2-752aa) was expressed with the N-terminal 10xHis tag. The purity of this Mapt protein was greater than 95% by SDS-PAGE. The activity has been validated.
Tau protein is a highly soluble microtubule-associated protein (MAP) encoded by the Mapt gene. Tau protein is a basic protein. As a member of the MAPs family, tau protein mainly acts on the distal end of axons to maintain the stability and flexibility of microtubules. Tau protein interacts with Tubulin to stabilize microtubules, while driving Tubulin assembly within microtubules. Tau proteins control microtubule stability through isomerization and phosphorylation.
Tau protein is involved in regulating axonal transport and nuclear function to protect DNA integrity; interacts with actin cytoskeleton to promote actin filament formation; and regulates NMDA receptor signaling pathway by interacting with Fyn.
Phosphorylation of tau is regulated by a number of kinases, including PKN, serine/threonine kinases, whose activation causes microtubule tissue disruption. The accumulation of hyperphosphorylated tau in neurons will cause neurofibrillary degeneration, which is associated with various neurodegenerative diseases such as AD and PD.
Datasheet & COA：

Please contact us to get it.

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靶點詳情

功能：

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
亞細胞定位：

Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted.
組織特異性：

Expressed in neurons. The larger forms (isoform tau-A and isoform tau-B) are preferentially expressed in the peripheral nervous system while the other are expressed in the central nervous system. Low amounts of the larger forms are also found in limited a
數據庫鏈接：

STRING: 10116.ENSRNOP00000006856

UniGene: Rn.2455