Recombinant Mouse G-protein coupled receptor 143 (Gpr143)

in vitro E.coli expression system

中文名稱(chēng)：

小鼠Gpr143重組蛋白
貨號(hào)：

CSB-CF009758MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

Gpr143
Uniprot No.：

P70259
別名：

Gpr143; Oa1; G-protein coupled receptor 143; MOA1; Ocular albinism type 1 protein homolog
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full length protein
表達(dá)區(qū)域：

1-405
氨基酸序列

MASPRLGIFCCPTWDAATQLVLSFQPRVFHALCLGSGTLRLVLGLLQLLSGRRSVGHRAP ATSPAASVHILRAATACDLLGCLGIVIRSTVWIAYPEFIENISNVNATDIWPATFCVGSA MWIQLLYSACFWWLFCYAVDVYLVIRRSAGRSTILLYHIMAWGLAVLLCVEGAVMLYYPS VSRCERGLDHAIPHYVTTYLPLLLVLVANPILFHKTVTSVASLLKGRKGVYTENERLMGA VIKTRFFKIMLVLIACWLSNIINESLLFYLEMQPDIHGGSLKRIQNAARTTWFIMGILNP AQGLLLSLAFYGWTGCSLDVHPPKMVIQWETMTASAAEGTYQTPVRSCVPHQNPRKVVCV GGHTSDEVLSILSEDSDASTVEIHTATGSCNIKEVDSISQAQGEL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.
基因功能參考文獻(xiàn)：
1. OA1 expression in both neuronal and non-neuronal tissues was examined by immunohistochemical analyses, results suggested that OA1 may modulate the monoaminergic functions in both peripheral and central nervous systems PMID: 25601010
2. Melanosome-autonomous regulation of size and number: the OA1 receptor sustains PMEL expression. PMID: 24650003
3. These results identify the Oa1 transducer Galphai3 as the first downstream component in the Oa1 signaling pathway. PMID: 21931697
4. OA1 interacts with MART-1 at early stages of melanogenesis to control melanosome identity and composition. PMID: 19717472
5. Asparagine at amino acid 106 is essential for N-glycosylation of the Oa1 protein. Mutation at amino acid 106 that eliminated glycosylation did not affect the endo/lysosomal distribution of Oa1 protein in cells. PMID: 11775061
6. the ocular albinism type 1 gene expression is controlled by microphthalmia transcription factor (Mitf) PMID: 15254223
7. The findings indicate that Oa1 is involved in the regulation of melanosome maturation at two steps, Oa1 controls the abundance of melanosomes in RPE cells and has a function in the maintenance of a correct melanosomal size. PMID: 16303920
8. G alpha i3, like Oa1, plays important role in melanosome biogenesis. Common Oa1-G alpha i3 signaling pathway may ultimately affect axonal growth through optic chiasm. PMID: 18378571
9. data point to defective regulation of organelle transport in pigment cells in absence of OA1; results enlighten novel function for OA1 in pigment cells & suggest ocular albinism type 1 may result from a different mechanism than previously thought PMID: 18697795
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亞細(xì)胞定位：

Melanosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor OA family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:18241

STRING: 10090.ENSMUSP00000026383

UniGene: Mm.5157