Recombinant Human Claudin-6 (CLDN6)-VLPs (Active)

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品名簡稱：

Recombinant Human CLDN6 protein-VLPs (Active)
貨號：

CSB-MP005508HU(A4)
說明書：

MSDS Datasheet
規(guī)格：

￥5000
圖片：
The presence of VLP-like structures was confirmed by TEM

Human CLDN6 Monoclonal Antibody (CSB-RA005508MA1HU) captured on Protein A Chip can bind Human CLDN6 Full Length VLP Protein with an affinity constant of 6.58 nM as detected by MetaSPR Assay (WeSPR^TM 200).

The purity of VLPs was greater than 95% as determined by SEC-HPLC Biological Activity Assay

CSB-MP005508HU(A4) is detected by Mouse anti-6*His monoclonal antibody.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/ml can bind Anti-CLDN6/9 recombinant antibody(CSB-RA005508MA1HU). The EC₅₀ is 1.334-1.504 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/ml can bind Anti-CLDN6 recombinant antibody(CSB-RA005508MA2HU). The EC₅₀ is 2.920-3.812 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/ml can bind Anti-CLDN6/9 recombinant antibody(CSB-RA005508MA3HU). The EC₅₀ is 3.482-4.097 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/ml can bind Anti-CLDN6 recombinant antibody(CSB-RA005508MA4HU). The EC₅₀ is 2.866-3.739 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay
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靶點詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SEC-HPLC.
內(nèi)毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

①Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/mL can bind Anti-CLDN6/9 recombinant antibody(CSB-RA005508MA1HU). The EC50 is 1.334-1.504 ng/mL.The VLPs (CSB-MP3838) is negative control.
②Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/mL can bind Anti-CLDN6 recombinant antibody(CSB-RA005508MA2HU). The EC50 is 2.920-3.812 ng/mL.The VLPs (CSB-MP3838) is negative control.
③Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/mL can bind Anti-CLDN6/9 recombinant antibody(CSB-RA005508MA3HU). The EC50 is 3.482-4.097 ng/mL.The VLPs (CSB-MP3838) is negative control.
④Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/mL can bind Anti-CLDN6 recombinant antibody(CSB-RA005508MA4HU). The EC50 is 2.866-3.739 ng/mL.The VLPs (CSB-MP3838) is negative control.
基因名：

CLDN6
Uniprot No.：

P56747
別名：

(Skullin)
分子結(jié)構(gòu)：
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

Mammalian cell
分子量：

24.8 kDa
表達區(qū)域：

1-220aa
氨基酸序列

MASAGMQILGVVLTLLGWVNGLVSCALPMWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLVALFGLLVYLAGAKCTTCVEEKDSKARLVLTSGIVFVISGVLTLIPVCWTAHAIIRDFYNPLVAEAQKRELGASLYLGWAASGLLLLGGGLLCCTCPSGGSQGPSHYMARYSTSAPAISRGPSEYPTKNYV
蛋白標簽：

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

"The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant)."
產(chǎn)品描述：

This Human Claudin-6 (CLDN6) recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human CLDN6 (1-220aa) was expressed with the C-terminal 10xHis tag. The activity was validated by its binding ability in a functional ELISA. This Human CLDN6 recombinant protein was developed through the Virus-Like Particles (VLPs) Platform. It is a four-pass transmembrane protein.
CLDNs are a class of TJ (Tight junctions) transmembrane proteins. From the perspective of spatial structure, most CLDNs contain four transmembrane regions and two extracellular loops, and an intracellular loop with amino and carboxyl termini located in the cytoplasm. Crystal structures of some CLDNs reveal a unique protein fold of the left-handed helical bundle and the four transmembrane helices of the extracellular caudal domain.
CLDN6 can activate cell adhesion signaling and modulate the activity of nuclear receptors. CLDN6 is expressed in a variety of embryonic epithelia, induces the formation and polarity of epithelial cell junctions, and is involved in the differentiation of stem cells into the epithelium. Collectively, CLDN6 is an important part of the CLDN family and plays an important role in maintaining the function of TJs. CLDN6 is specifically expressed in various cancers such as ovarian cancer, testicular cancer, hepatocellular carcinoma and lung adenocarcinoma, differentially expressed on cancer cells, and almost undetectable in adult normal tissues, making it a potential target point.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

平均分:

5.0分 - 1 個評價

實驗應(yīng)用：藥物相關(guān)研究

產(chǎn)品評價： CLDN6是CLDN家族的重要成員，在維持TJs的功能中起著重要作用。CLDN6在卵巢癌、睪丸癌、肝細胞癌、肺腺癌等多種癌癥中均有特異性表達，在癌細胞上有差異表達，在成人正常組織中幾乎檢測不到，是潛在的靶點。CLDN6是一個4次跨膜的蛋白。CUSA Bio通過其特有的Vlp平臺表達的全長重組人CLDN6蛋白，具有較好的生物活性，我使用CSB-MP005508HU(A4)產(chǎn)品進行ELISA實驗，EC50為1.726-3.525 ng/ml，后續(xù)實驗進展順利，對我的研究幫助很大，考慮繼續(xù)大量采購！

By 唐老師

靶點詳情

功能：

Plays a major role in tight junction-specific obliteration of the intercellular space.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
基因功能參考文獻：
1. Antibodies recognizing native CLDN6 as displayed on cell surfaces and mediating complement-dependent cytotoxicity were elicited in vaccinated animals. The data suggest applications of CLDN6 displaying Virus-like particles in cancer immunotherapy. PMID: 29131519
2. these results suggest that Helicobacter pylori lipopolysaccharide induces TLR2 expression in the gastric adenocarcinoma cells, and that the longer the exposure to lipopolysaccharide, the greater the expression of TLR2 in the cell membrane; consequently the expression of claudin-4, -6, -7 and -9 also increases PMID: 29031421
3. Study provides evidence that high expression of CLDN6 confers chemoresistance on breast cancer which is mediated by GSTP1, the activity of which is regulated by p53. PMID: 29116019
4. CLDN6 enhances the chemoresistance to ADM via activating the AF-6/ERK signaling pathway and up-regulating cancer stem cell characters in MDAMB231 cells. PMID: 29159771
5. we demonstrated that the downregulation of CLDN6 is regulated through promoter methylation by DNMT1, which depends on the SMAD2 pathway, and that CLDN6 is a key regulator in the SMAD2/DNMT1/CLDN6 pathway to inhibit EMT, migration and invasion of breast cancer cells PMID: 28867761
6. In conclusion, this information from bioinformatics analysis will help future attempts to better understand CLDN6 regulation and functions. PMID: 28656265
7. high expression of CLDN 6 was observed in approx. 65% of the myxofibrosarcomas, whereas the benign soft tissue tumors did not show a high expression of CLDN 6. The expression of CLDN 6 in the myxofibrosarcomas was significantly higher than those of other tumor specimens. Among the myxofibrosarcomas, the high expression of CLDN 6 was correlated with high FNCLCC grades and high AJCC stages. PMID: 28476380
8. Results show that DNA methylation down-regulates CLDN6 expression through MeCP2 binding to the CLDN6 promoter, deacetylating H3 and H4, and altering chromatin structure, consequently promoting migratory and invasive phenotype in breast cancer cells. PMID: 27461117
9. Cldn6 was decreased in alveolar type II-like epithelial cells (A549) and primary small airway epithelial cells when exposed to cigarette smoke ext PMID: 27982694
10. suggest that claudin-6 induces MMP-2 activation through claudin-1 membrane expression PMID: 27914788
11. Data show that claudin-6 (CLDN6) R209Q and occludin (OCLN) P24A mutations do not affect HCV pseudoparticles (HCVpp) entry. PMID: 26561856
12. The expression of ASK1 is correlated with the level of claudin-6 in cervical carcinoma cells and tissues. PMID: 26191261
13. High levels of CLDN6 are associated with non-small-cell lung cancer. PMID: 24710653
14. The expression of claudin-6 was down regulated in gastric cancer tissue. PMID: 23919729
15. Only some hepatitis C virus strains efficiently use CLDN6 for infection. PMID: 23775920
16. This work provides a proof of concept for the use of Claudin-6 to eliminate residual undifferentiated human pluripotent stem cells from culture. PMID: 23778593
17. Although claudin-6 and claudin-9 can serve as entry factors in cell lines, hepatitis C virus infection into human hepatocytes is not dependent on claudin-6 and claudin-9. PMID: 23864633
18. ASK1 signal may play a positive role in the inhibitory effect of claudin-6 in breast cancer. PMID: 22925655
19. Our results show that claudin-6 protein is significantly down-regulated in breast invasive ductal carcinomas PMID: 22455563
20. CLDN6 is not a specific biomarker for atypical teratoid rhabdoid tumors as it is expressed in a variety of other pediatric CNS and soft tissue tumors. PMID: 21989342
21. 17beta-E2 might regulate the expression of claudin-6 and inhibit the proliferation and migration of MCF-7 cells. PMID: 20388399
22. Increased expression of claudin-6, claudin-7, or claudin-9 is sufficient to enhance tumorigenic properties of a gastric adenocarcinoma cell line. PMID: 20874001
23. CLDN6 may be a useful positive marker to help further identify atypical teratoid/rhabdoid tumors for diagnostic and treatment purposes PMID: 19220299
24. Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis PMID: 19960275
25. The up-regulation of claudin-6 expression in MCF-7 breast cancer cells suppresses their malignant phenotypes with a correlation with the restoration of tight junction integrity. PMID: 20367941
26. claudin-6, downregulates the malignant phenotype of breast carcinoma. PMID: 20215972
27. Claudin 6 was not found in epithelioid glioblastomas or rhabdoid glioblastomas. PMID: 20118769
28. CLDN6 and CLDN9, but not CLDN1, are expressed in peripheral blood mononuclear cells, an additional site of HCV replication. PMID: 17804490
29. claudin-6 and claudin-9 expressed in CD81+ cells also enable the entry of HCV pseudoparticles derived from six of the major genotypes. PMID: 18234789
30. CLDN6, clustered with CLDN9 at human chromosome 16p13.3, is a four-transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C. PMID: 12736707
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亞細胞定位：

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
蛋白家族：

Claudin family
組織特異性：

Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.
數(shù)據(jù)庫鏈接：

HGNC: 2048

OMIM: 615798

KEGG: hsa:9074

STRING: 9606.ENSP00000328674

UniGene: Hs.533779