Recombinant Human Tumor necrosis factor ligand superfamily member 8 (TNFSF8), partial (Active)

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中文名稱：

人TNFSF8重組蛋白
品名簡稱：

Recombinant Human TNFSF8 protein, partial (Active)
貨號：

CSB-MP023996HU1
說明書：

MSDS Datasheet
規格：

￥816
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized CD30(CSB-MP023983HU1h6) at 5 μg/ml can bind human CD30L, the EC₅₀ is 9.531-12.49 ng/ml. Biological Activity Assay
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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized CD30(CSB-MP023983HU1h6) at 5 μg/ml can bind human CD30L, the EC₅₀ is 9.531-12.49 ng/ml.
基因名：

TNFSF8
Uniprot No.：

P32971
別名：

TNFSF8; CD30L; CD30LG; Tumor necrosis factor ligand superfamily member 8; CD30 ligand; CD30-L; CD antigen CD153
分子結構：
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

21.8 kDa
表達區域：

63-234aa
氨基酸序列

QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Human TNFSF8 amino acids Gly21-Glu147 with N-terminal 6xHis-tag were expressed in mammalian cells. The resulting product is the recombinant human TNFSF8 protein. This TNFSF8 protein was validated by SDS-PAGE analysis, and its purity is greater than 75%. It migrated to the molecular mass band of about 22 kDa on the gel. Its endotoxin level is less than 1.0 EU/ug determined by the LAL method. This TNFSF8 protein can bind to the immobilized CD30 in the functional ELISA, with an EC₅₀ of 9.531-12.49 ng/ml. It shows the TNFSF8 is an active protein. And it is available now.

TNFSF8, also called CD30L or CD153, is primarily expressed on activated T and B cells eosinophils, and macrophages. CD30L/CD30 signaling is involved in Th1, Th2, Th17 cell responses, and Th1-, and Th2- associated diseases.
Datasheet & COA：

Please contact us to get it.

產品評價

平均分:

5.0分 - 1 個評價

實驗應用：結合實驗/蛋白間互作

產品評價： TNFSF8也被稱為CD30L或CD153，主要在活化的T細胞和B細胞嗜酸性粒細胞和巨噬細胞上表達。CD30L/CD30信號通路參與Th1, Th2, Th17細胞反應以及Th1-和Th2-相關疾病。CD30L和CD30有較好的結合活性，通過ELISA方法高通量篩選，建立起了穩定的篩選體系。

By 溫老師

靶點詳情

功能：

Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
基因功能參考文獻：
1. circulant sCD30L is functionally active and that it may favor persistence of active inflammation by inducing apoptosis of CD30(+)T cells, known to down-modulate inflammation in rheumatoid synovitis. PMID: 24447865
2. TNFSF8 is an important leprosy T1R susceptibility gene. PMID: 25320285
3. The heritability of IgA levels is moderate and can partly be attributable to common variation in the CD30L locus. PMID: 24676358
4. The TNFSF8 polymorphisms rs927374 and rs2295800 were associated with neutrophil count. This finding suggests that post-MI inflammatory response is genetically modulated. PMID: 22033252
5. Positional candidate gene screening in the SPA2 locus allowed us to identify and replicate an association between a rare SNP located in TNFSF8 and spondylarthritis. PMID: 21480186
6. a possible role of novel TNFSF8 variants in susceptibility to lung cancer. PMID: 21292647
7. capability to up-regulate expression of CD30, release of soluble CD30 and production of IL-4 in pre-activated T cells upon co-culture PMID: 11728464
8. Mast cells were found to be the predominant CD30 ligand-positive (CD30L-positive) cell in the chronic inflammatory skin diseases psoriasis and atopic dermatitis. PMID: 16964309
9. CD153 antigen was expressed by synovial mast cells, and correlated with serum levels, in Rheumatoid Arthritis patients PMID: 19208589
10. Single nucleotide polymorphism in TNFSF8 gene is associated with bone disease in myeloma. PMID: 19657367
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亞細胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

HGNC: 11938

OMIM: 603875

KEGG: hsa:944

STRING: 9606.ENSP00000223795

UniGene: Hs.494901