GAPDH
GAPDH，即甘油醛-3-磷酸脫氫酶，別名G3PDH。GAPDH是一種多功能酶，主要參與糖酵解過程中的關鍵步驟，即催化甘油醛-3-磷酸轉化為1,3-二磷酸甘油酸，并在此過程中釋放能量（ATP）。GAPDH在生物界中非常保守，單體分子量在30kD-40kD之間，天然狀態的GAPDH主要以四聚體形式存在。除了在能量代謝中的作用，GAPDH還具有多種其他功能，包括催化微管聚合、參與蛋白磷酸化修飾、膜融合和膜轉運、促進細胞凋亡、調節蛋白質的表達、參與DNA損傷修復、作為轉鐵蛋白受體等。GAPDH不僅在糖代謝中發揮關鍵作用，其異常表達與多種疾病相關，包括神經退行性疾病、糖尿病、癌癥等，且在細胞增殖和腫瘤發生中可能扮演重要角色。
熱銷產品
Recombinant Human Epidermal growth factor receptor (EGFR), partial (Active) (CSB-MP007479HU)
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti-EGFR recombinant antibody, the EC50 of human EGFR protein is 2.867-3.571 ng/ml.
Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9nM as detected by LSPR Assay.
Activity
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Cetuximab, the EC50 of human EGFR protein is 0.6919-1.047 ng/ml.
The purity of EGFR was greater than 95% as determined by SEC-HPLC
EGFR Recombinant Monoclonal Antibody (CSB-RA159341MA1HU)
驗證數據
IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA159341MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
GAPDH Antibodies
GAPDH for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA009232GA01HU | GAPDH Antibody | Human,Mouse,Rat,Pig | ELISA,WB,IHC,IF |
| CSB-PA00025B0Rb | GAPDH Antibody, HRP conjugated | Human | ELISA |
| CSB-PA00025D0Rb | GAPDH Antibody, Biotin conjugated | Human | ELISA |
| CSB-MA000071M0m | GAPDH Monoclonal Antibody | Human, Rat, Rabbit | ELISA, WB, IHC, IP, IF, FC |
| CSB-PA115482 | GAPDH Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA000351 | GAPDH Antibody | Human,Mouse,Rat,Rabbit,Chicken,Monkey,Sheep,X | WB,IHC |
| CSB-PA000364 | GAPDH Antibody | Zebrafish | WB |
| CSB-PA004666 | GAPDH Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA080240 | GAPDH Antibody | Human,Mouse,Rat | WB, IHC |
| CSB-PA779821 | GAPDH Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA00025A0Rb | GAPDH Antibody | Human | ELISA, WB, IHC, IF |
| CSB-PA00029A0Rb | GAPDH Antibody | Human, Mouse | ELISA, WB, IHC, IF |
| CSB-PA00029B0Rb | GAPDH Antibody, HRP conjugated | Human | ELISA |
| CSB-PA00029C0Rb | GAPDH Antibody, FITC conjugated | Human | |
| CSB-PA00029D0Rb | GAPDH Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA009232A0HU | GAPDH Recombinant Monoclonal Antibody | Human, Mouse | ELISA, WB |
| CSB-MA000071M1m | GAPDH Monoclonal Antibody | Human, Mouse, Rabbit | ELISA, WB, IHC, IP, IF |
| CSB-MA000071M2m | GAPDH Monoclonal Antibody | Human, Rat, Rabbit, Mouse | ELISA, WB, IHC, IP, IF |
| CSB-RA009232MA1HU | GAPDH Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
GAPDH for Chicken
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA009232HC01CH | GAPDH Antibody, FITC conjugated | Chicken | |
| CSB-PA009232HA01CH | GAPDH Antibody | Chicken | ELISA |
| CSB-PA009232HD01CH | GAPDH Antibody, Biotin conjugated | Chicken | ELISA |
GAPDH for H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh, Insect, Yeast
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-MA000184 | GAPDH Monoclonal Antibody | H,R,M,Mk,Dg,C,Hm,Rb,Pg,Sh,Insect,Yeast | ELISA,WB,IHC |
GAPDH for H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-MA000195 | GAPDH Monoclonal Antibody | H,R,M,Mk,Dg,C,Hm,Rb,Pg,Sh | ELISA,WB |
GAPDH for Cricetulus griseus
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA009232HA01DXU | GAPDH Antibody | Cricetulus griseus | ELISA, WB |
| CSB-PA009232HB01DXU | GAPDH Antibody, HRP conjugated | Cricetulus griseus | ELISA |
| CSB-PA009232HC01DXU | GAPDH Antibody, FITC conjugated | Cricetulus griseus | |
| CSB-PA009232HD01DXU | GAPDH Antibody, Biotin conjugated | Cricetulus griseus | ELISA |
GAPDH Proteins
GAPDH Proteins for Cavia porcellus (Guinea pig)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232GU CSB-EP009232GU CSB-BP009232GU CSB-MP009232GU CSB-EP009232GU-B |
Recombinant Guinea pig Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Bos taurus (Bovine)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232BO CSB-EP009232BO CSB-BP009232BO CSB-MP009232BO CSB-EP009232BO-B |
Recombinant Bovine Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232MO CSB-EP009232MO CSB-BP009232MO CSB-MP009232MO CSB-EP009232MO-B |
Recombinant Mouse Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Oryctolagus cuniculus (Rabbit)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232RB CSB-EP009232RB CSB-BP009232RB CSB-MP009232RB CSB-EP009232RB-B |
Recombinant Rabbit Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Gallus gallus (Chicken)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232CH CSB-BP009232CH CSB-MP009232CH |
Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast Baculovirus Mammalian cell |
| CSB-EP009232CH | Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | E.coli |
| CSB-EP009232CH-B | Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial,Biotinylated | E.coli |
GAPDH Proteins for Sus scrofa (Pig)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232PI CSB-EP009232PI CSB-BP009232PI CSB-MP009232PI CSB-EP009232PI-B |
Recombinant Pig Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232HU CSB-BP009232HU CSB-MP009232HU CSB-EP009232HU-B |
Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP009232HU1 | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial | E.coli |
| CSB-EP009232HU | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | E.coli |
| CSB-EP009232HUl1 | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | E.coli |
| CSB-EP009232HUa0 | Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | E.coli |
GAPDH Proteins for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232OEI CSB-EP009232OEI CSB-BP009232OEI CSB-MP009232OEI CSB-EP009232OEI-B |
Recombinant Oncorhynchus mykiss Glyceraldehyde-3-phosphate dehydrogenase (gapdh) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Columba livia (Domestic pigeon)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232DVK CSB-EP009232DVK CSB-BP009232DVK CSB-MP009232DVK CSB-EP009232DVK-B |
Recombinant Columba livia Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Meleagris gallopavo (Common turkey)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232MQV CSB-EP009232MQV CSB-BP009232MQV CSB-MP009232MQV CSB-EP009232MQV-B |
Recombinant Meleagris gallopavo Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Mustela putorius furo (European domestic ferret) (Mustela furo)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232MOH CSB-EP009232MOH CSB-BP009232MOH CSB-MP009232MOH CSB-EP009232MOH-B |
Recombinant Mustela putorius furo Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232DXU CSB-BP009232DXU CSB-MP009232DXU CSB-EP009232DXU-B |
Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP009232DXU | Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial | E.coli |
GAPDH Proteins for Xenopus laevis (African clawed frog)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232XBE CSB-EP009232XBE CSB-BP009232XBE CSB-MP009232XBE CSB-EP009232XBE-B |
Recombinant Xenopus laevis Glyceraldehyde-3-phosphate dehydrogenase (gapdh) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Mesocricetus auratus (Golden hamster)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232MRG CSB-EP009232MRG CSB-BP009232MRG CSB-MP009232MRG CSB-EP009232MRG-B |
Recombinant Mesocricetus auratus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232RA CSB-EP009232RA CSB-BP009232RA CSB-MP009232RA CSB-EP009232RA-B |
Recombinant Rat Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232PEL CSB-EP009232PEL CSB-BP009232PEL CSB-MP009232PEL CSB-EP009232PEL-B |
Recombinant Pelodiscus sinensis Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Pongo abelii (Sumatran orangutan)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232PYX CSB-EP009232PYX CSB-BP009232PYX CSB-MP009232PYX CSB-EP009232PYX-B |
Recombinant Pongo abelii Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232DIL CSB-EP009232DIL CSB-BP009232DIL CSB-MP009232DIL CSB-EP009232DIL-B |
Recombinant Danio rerio Glyceraldehyde-3-phosphate dehydrogenase (gapdh) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Felis catus (Cat) (Felis silvestris catus)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232CA CSB-EP009232CA CSB-BP009232CA CSB-MP009232CA CSB-EP009232CA-B |
Recombinant Cat Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Ovis aries (Sheep)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232SH CSB-EP009232SH CSB-BP009232SH CSB-MP009232SH CSB-EP009232SH-B |
Recombinant Sheep Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Canis familiaris (Dog) (Canis lupus familiaris)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232DO CSB-EP009232DO CSB-BP009232DO CSB-MP009232DO CSB-EP009232DO-B |
Recombinant Dog Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH Proteins for Glossina morsitans morsitans (Savannah tsetse fly)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP009232GGM CSB-EP009232GGM CSB-BP009232GGM CSB-MP009232GGM CSB-EP009232GGM-B |
Recombinant Glossina morsitans morsitans Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
GAPDH ELISA Kit
GAPDH ELISA Kit for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-E13911h | Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH/G3PDH) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 7.81 pg/mL |
