Recombinant Rat Insulin receptor substrate 1 (Irs1), partial

中文名稱：

Recombinant Rat Insulin receptor substrate 1 (Irs1), partial
品名簡稱：

Recombinant Rat Irs1 protein, partial
貨號：

CSB-EP011829RA
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Irs1
Uniprot No.：

P35570
別名：

pp185
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
來源：

E.coli
分子量：

34.4 kDa
表達區(qū)域：

12-259aa
氨基酸序列

DVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKAHHDGAGGGCGGSCSGSSGVGEAGEDLSYDTGPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
基因功能參考文獻：
1. EAF exerted its effect by modulating insulin signalling, potentially via IRS1/AKT activation. PMID: 29248832
2. Treatment by tanshinone I lowered the levels of IL-6 and TNF-alpha, decreased nuclear translocation of NF-kappaB as well as phosphorylation of Ser307 in IRS-1. PMID: 28651236
3. This study determined if dietary restriction (DR) protects against hypoxic-ischemia (HI) in the neonatal brain via insulin receptor substrate-1 (IRS-1)/Akt pathway-mediated downregulation of p53 in the neurovascular unit. PMID: 26111627
4. the increased IRS1 expression may mediate the insulin-sensitizing effects of sodium butyrate. PMID: 27619406
5. results suggest that upregulation of the INSR on T cells following activation is required for efficient adaptive immunity PMID: 28115529
6. The results suggest that high glucose compromises the insulin signaling pathway in the glomerulus, promoting a proapoptotic environment, with a possible critical step for this malfunction lying at the level of IRS-1 phosphorylation. PMID: 27434075
7. Under insulin stimulation, and in spite of the less activated mTOR function in high altitude rats, low altitude rats had higher glycogen content and reduced levels of gluconeogenic enzymes with a more enhanced insulin signaling, mainly due to higher levels of phosphorylated IRS1. PMID: 28084108
8. Chronic periapical lesions are associated with decreased insulin signaling - evaluated according to insulin receptor substrate (pp185-insulin receptor substrate 1 [IRS-1]/insulin receptor substrate 2 [IRS-2]) tyrosine phosphorylation status, IRS-1 serine phosphorylation status, and IRS-1 and IRS-2 content - in the skeletal muscle of rats PMID: 26027876
9. Data suggest FSH and IGF1 (insulin-like growth factor-1) PI3K signaling pathways in granulosa cells converge at IRS1 (insulin receptor substrate 1), requiring PKA (cyclic AMP-dependent protein kinase) and PP1 (protein phosphatase 1) to regulate IRS1. PMID: 26702053
10. IRS-2 was one of the targets of miR-126. MiR-126 inhibited the beta-cell proliferation through IRS-2 instead of IRS-1. MiR-126 may take part in the glucose homeostasis both through its target IRS-2 and IRS-1. PMID: 26919700
11. These results suggest that the GSK-3beta-IRS1 pathway may play a significant role in Wnt-mediated central blood pressure regulation. PMID: 25883115
12. overexpression of IRS1 stimulated osteoblast proliferation, as evidenced by an increase in the number of cells in the S phase compared to controls. PMID: 25372512
13. Insulin receptor substrate-1 (IRS-1) is recruited to interact with the IR-catalyzed phospho-tyrosine cav-2, which facilitates IRS-1 association with and activation by IR to initiate IRS-1-mediated downstream signaling. PMID: 25667086
14. Serine 307 on IRS-1 is indispensable for SOCS3 and TNF-alpha signaling in the retinal neuroglial cell line. PMID: 25352752
15. insulin-resistant status of rats induced by dexamethasone administration have PKB and IRS-1 activity attenuated in epididymal fat without increases in the phosphorylation of the proinflammatory signals JNK and IKKbeta PMID: 25268311
16. Daata indicate that insulin receptor substrate 1 IRS1 Ser(P)/Thr(P) is an integrated response to insulin stimulation and metabolic stress, which associates with reduced Tyr(P)(Irs1). PMID: 24652289
17. Suggest that oleanolic acid supplement ameliorates fructose-induced adipose tissue insulin resistance in rats via the IRS-1/phosphatidylinositol 3-kinase/Akt pathway. PMID: 24704288
18. Dietary high-fructose corn syrup causes vascular insulin resistance and endothelial dysfunction through attenuating IRS-1 and eNOS expressions as well as increasing iNOS in rats. PMID: 23872130
19. Data indicate that hyperglycemia seems to inhibit the interaction between insulin receptor substrate (IRS1) and insulin receptor (IRbeta). PMID: 24462861
20. Taken together, our findings demonstrate that activation of the ERK pathway in sensory neurons as a consequence of mTORC1 inhibition leads to the development of pain. PMID: 23607966
21. Nexilin, a cardiomyopathy-associated F-actin binding protein, binds and regulates IRS1 signaling in skeletal muscle cells. PMID: 23383252
22. Data suggest that down-regulation of insulin signaling pathway (Irs1, Irs2, and insulin-like growth factor-1) in type II diabetic osteoporosis model occurs either sooner or more dramatically in bone as compared to liver, kidney or skeletal muscle. PMID: 22820932
23. Induction of streptozotocin diabetes resulted in decreased level of GLP-1R and increased levels of IRS-1 and Raf-1 in the liver. PMID: 22983684
24. phosphorylation of IRS1 is governed by a small plasma membrane-localized pool of IR with an early peak, or (ii) inhibition of the IR-catalyzed phosphorylation of IRS1 by negative feedback PMID: 22248283
25. Adiponectin may decrease tyrosine phosphorylation of IRS-1 via the IKK/NFkappaB pathway and inhibit insulin signaling pathway in the liver. PMID: 21602124
26. Increased IKKalpha/beta and JNK serine phosphorylation may contribute to increasing IRS-1 serine phosphorylation, while concurrent co-localization of SOCS-3 with both IR-beta and IRS-1 may prevent IRS-1 from interacting with IR-beta; these 2 mechanisms may independently contribute to impairing insulin-stimulated PI3-K activation in the skeletal muscle of the obese Zucker rat. PMID: 22982470
27. the importance of IRS-1 in beta-adrenergic receptor signaling in the prevention of cell death in retinal Muller cells is suggested PMID: 22328823
28. The insulin resistance in L6 cells induced by TNF-alpha might be attenuated by acetyl-L-carnitine via decreasing the expression of Ser307 phosphorylation of IRS-1. PMID: 20459025
29. These findings suggest that improvements in glucose tolerance and insulin resistance following Roux-en-Y gastric bypass surgery are associated with upregulation of IRS-1. PMID: 22001674
30. Foxo1 protein was excluded from the nuclei in L6-mIRS1 cells, whereas Foxo1 was localized in the nuclei in control L6 cells during induction of differentiation. PMID: 21991327
31. We conclude that phosphorylation of Ser307, Ser302, and Ser632 of IRS-1 is not involved in ANG II-mediated IRS-1 degradation PMID: 21900690
32. sex steroid deficiency induces impaired glucose oxidation in insulin responsive tissues, which is mediated through reduced IR expression, and increased IRS-1 serine phosphorylation PMID: 21301931
33. Our findings suggest an interaction between vitamin D(3) and muscarinic M3 receptors in regulating insulin secretion from pancreas. PMID: 20655720
34. Protein, but not mRNA levels of IRS1, was decreased only in the glomeruli of streptozotocin-diabetic rats likely due to increased ubiquitination. PMID: 21228767
35. resistin induces cardiac hypertrophy and myocardial insulin resistance, possibly via the AMPK/mTOR/p70(S6K) and apoptosis signal-regulating kinase 1/JNK/IRS1 pathways. PMID: 21478152
36. Adiponectin receptors and IRS1 might be crucially important regulators for the synthesis and oxidation of fatty acids in the liver of Nonalcoholic steatohepatitis PMID: 20846698
37. iNOS-mediated tyrosine nitration of IRS-1 is a key mechanism of skeletal muscle insulin resistance in endotoxemia PMID: 21206533
38. Over-expression of NYGGF4 inhibits glucose transport in skeletal myotubes by blocking the IRS1/PI3K/AKT insulin pathway PMID: 21185755
39. Chronic ethanol intake could induce insulin resistance and down-regulated the expression of IR, IRS-land PI-3K mRNA in skeletal muscle. PMID: 17555093
40. Insulin resistance was associated with enhanced Ser phosphorylation of IRS-1, which impaired its interaction with its downstream target PKB/Akt. PMID: 19563078
41. The decreased of IR, IRS-1, IRS-2 mRNA expression of liver may be involved in the molecular mechanism of the effects of ethanol on insulin insensitivity. PMID: 16921752
42. Chromium supplementation can increase the expression level of IRS-1 mRNA of skeletal muscles in diabetic rats. PMID: 15862035
43. Bushen Huoxue Huayu Recipe can increase the expression of IRS-1 and phosphorylation of tyrosine in adipose tissue. PMID: 18559242
44. Insulin-like growth factor-I-stimulated insulin receptor substrate-1 negatively regulates Src homology 2 domain-containing protein-tyrosine phosphatase substrate-1 function in vascular smooth muscle cells. PMID: 20207740
45. The reduced expressions of IRS-1 and IRS-2 caused lower expression of hepatic IGF-1 in the adult male rats with intrauterine growth retardation. PMID: 19088829
46. Loss of inhibitory insulin receptor substrate-1 phosphorylation is an early event in mammalian target of rapamycin-dependent endometrial hyperplasia and carcinoma. PMID: 20179297
47. In summary, we show that, in a rodent model of T2DM, voluntary exercise decreases circulating markers of inflammation and oxidative stress and lowers hepatic JNK activation and Ser(307)-phosphorylated insulin receptor substrate-1. PMID: 19996384
48. There was no significant difference of IRS-1 expression among three rat age groups. PMID: 19626997
49. The sites of O-linked N-acetylglucosamine modification of rat and human IRS-1, were identified. PMID: 19671924
50. Expression of IRS1 can be directly activated by beta-catenin. PMID: 19843521
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數(shù)據(jù)庫鏈接：

KEGG: rno:25467

STRING: 10116.ENSRNOP00000019579

UniGene: Rn.10476