Recombinant Rat Huntingtin-associated protein 1 (Hap1)

中文名稱：

大鼠Hap1重組蛋白
貨號：

CSB-YP010129RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Hap1重組蛋白
貨號：

CSB-EP010129RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Hap1重組蛋白
貨號：

CSB-EP010129RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Hap1重組蛋白
貨號：

CSB-BP010129RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Hap1重組蛋白
貨號：

CSB-MP010129RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Hap1
Uniprot No.：

P54256
別名：

Hap1; Huntingtin-associated protein 1; HAP-1
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full length protein
表達區域：

1-629
氨基酸序列

MRPKDQVQSS AGDGTGSGDP ATGTPTTQPA ADPAPEPSAE PKPAPAQGTG SGQKSGSRTK TGGSFCRSRI RGDSDAPWTR YIFQGPYGPR ATGLGTGRAE GIWKTPAAYI GRRPGVSGPE RAAFIRELQE ALCPNPLPRK KITEDDIKVM LYLLEEKERD LNTAARIGQS LVKQNSVLME ENNKLETMLG SAREEILHLR KQVNLRDDLL QLYSDSDDDE EDEEDEEEEE GEEEEREGQR DQDQQHDHPY GAPKPPPKAE TLHHCPQLEA LKQKLKLLEE ENDHLREEAS HLDNLEDKEQ MLILECVEQF SEASQQMAEL SEVLVLRLEG YERQQKEITQ LQAEITKLQQ RCQSYGAQTE KLQQQLASEK GVHPESLRAG SHMQDYGSRP RERQEDGKSH RQRSSMPAGS VTHYGYSVPL DALPSFPETL AEELRTSLRK FITDPAYFME RCDTRCREER KKEQGTMPPP PVQDLKPPED FEAPEELVPE EELGAIEEVG TAEDGPAEET EQASEETEAW EEVEPEVDEA TRMNVVVSAL EASGLGPSHL DMKYVLQQLS NWQDAHSKRQ QKQKVVPKDS PAPQQQTNMG GGIVEQQPIV PTQDSQRLEE DRATHSPSAR EEEGPSGAT
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion; the function seems to involve HTT, DCTN1 and a complex with SORT1. Involved in APP trafficking and seems to facilitate APP anterograde transport and membrane insertion thereby possibly reducing processing into amyloid beta. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptors to synapses; the function is dependent on kinesin motor protein KIF5 and is disrupted by HTT with expanded polyglutamine repeat. Involved in regulation of autophagosome motility by promoting efficient retrograde axonal transport. Seems to be involved in regulation of membrane receptor recycling and degradation, and respective signal transduction, including GABA(A) receptors, tyrosine kinase receptors, EGFR, IP3 receptor and androgen receptor. Among others suggested to be involved in control of feeding behavior (involving hypothalamic GABA(A) receptors), cerebellar and brainstem development (involving AHI1 and NTRK1/TrkA), postnatal neurogenesis (involving hypothalamic NTRK2/TrkB), and ITPR1/InsP3R1-mediated Ca(2+) release (involving HTT and possibly the effect of mutant HTT). Via association with DCTN1/dynactin p150-glued and HTT/huntingtin involved in cytoplasmic retention of REST in neurons. May be involved in ciliogenesis. Involved in regulation of exocytosis. Isoform A but not isoform B seems to be involved in formation of cytoplasmic inclusion bodies (STBs). In case of anomalous expression of TBP, can sequester a subset of TBP into STBs; sequestration is enhanced by an expanded polyglutamine repeat within TBP.
基因功能參考文獻：
1. A key role for HAP1 protein was determined in the down-regulation Of GABA A receptor neurotransmission during cerebral ischemia. PMID: 26732589
2. Study demonstrated that HAP1 is abundantly expressed in spinal neurons of the somatosensory, viscerosensory, and autonomic regions but absent in somatomotor neurons, suggesting that the spinal motoneurons are, due to lack of putative HAP1 protectivity, more vulnerable to stresses in neurodegenerative diseases than other HAP1-expressing neurons probably involved in spinal sensory and autonomic functions. PMID: 27984179
3. results provide real-time evidence in living cells that HAP1 may help regulate transport of insulin-containing secretory granules along cortical actin filaments PMID: 25744490
4. sortilin stabilizes the proBDNF.HAP1 complex PMID: 21357693
5. Data suggest that HAP1-KIF5-dependent GABA(A)R trafficking is a fundamental mechanism controlling the strength of synaptic inhibition in the brain. PMID: 20152113
6. HAP1 is selectively expressed in beta-cells of rat pancreatic islets, suggesting the involvement of HAP1 in the regulation of cellular trafficking and secretion of insulin. PMID: 19901268
7. chromosome 10 mapping by radiation hybrid mapping and FISH PMID: 11701969
8. interacts with hepatocyte growth factor-regulated tyrosine kinase substrate and functions in endosomal trafficking PMID: 12021262
9. Huntingtin-associated protein-1 interacts with kinesin light chain and has a role in intracellular trafficking in neurons PMID: 16339760
10. HAP1 trafficking is critical for the stability of TrkA and neurite function, both of which can be attenuated by mutant huntingtin. PMID: 16738245
11. HAP1 interacts with Androgen Receptor through its ligand-binding domain in a polyQ-length-dependent manner and suppresses SBMA-mutant-AR-induced apoptosis. PMID: 16782802
12. phosphorylation-dependent interaction of HAP1A with 14-3-3 regulates HAP1 function by influencing its association with kinesin light chain and trafficking in neuronal processes. PMID: 17166838
13. HAP1-induced/immunoreactive inclusions correspond to the human placental antigen complex X-P-immunoreactive stigmoid bodies previously identified in the brain PMID: 17687563
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亞細胞定位：

Cytoplasm. Cell junction, synapse, presynapse. Cytoplasm, cytoskeleton. Cell projection, dendritic spine. Cell projection, dendrite. Cell projection, axon. Lysosome. Endoplasmic reticulum. Mitochondrion. Nucleus. Cytoplasmic vesicle, autophagosome. Early endosome. Cell projection, growth cone.; [Isoform B]: Cytoplasm.; [Isoform A]: Cell projection, growth cone. Cell projection, neuron projection. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cell junction, synapse, presynapse.
組織特異性：

In the brain, especially in the olfactory bulb and in the brain stem. No detectable expression in peripheral tissues such as lung, testis, spleen, and small intestine.
數據庫鏈接：

KEGG: rno:29430

STRING: 10116.ENSRNOP00000020603

UniGene: Rn.37430