Recombinant Human Mucin-17 (MUC17), partial (Active)

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中文名稱：

人MUC17重組蛋白
品名簡稱：

Recombinant Human MUC17 protein, partial (Active)
貨號：

CSB-MP727848HU
說明書：

MSDS Datasheet
規格：

￥1140
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human MUC17 at 2 μg/mL can bind Anti-MUC17 recombinant antibody (CSB-RA727848MA1HU), the EC₅₀ is 0.9057-1.259 ng/mL.
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產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Human MUC17 at 2 μg/mL can bind Anti-MUC17 recombinant antibody (CSB-RA727848MA1HU), the EC50 is 0.9057-1.259 ng/mL.
基因名：

MUC17
Uniprot No.：

Q685J3
別名：

(MUC-17)(Small intestinal mucin-3)(MUC-3)
分子結構：
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

32.0kDa
表達區域：

4131-4390aa
氨基酸序列

RTTTCFGDGCQNTASRCKNGGTWDGLKCQCPNLYYGELCEEVVSSIDIGPPETISAQMELTVTVTSVKFTEELKNHSSQEFQEFKQTFTEQMNIVYSGIPEYVGVNITKLRLGSVVVEHDVLLRTKYTPEYKTVLDNATEVVKEKITKVTTQQIMINDICSDMMCFNTTGTQVQNITVTQYDPEEDCRKMAKEYGDYFVVEYRDQKPYCISPCEPGFSVSKNCNLGKCQMSLSGPQCLCVTTETHWYSGETCNQGTQKSL
蛋白標簽：

C-terminal?10xHis-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

This Human MUC17 (Mucin-17) recombinant protein was produced in mammalian cell, where the gene sequence encoding human MUC17 (4131-4390aa) was expressed with the C-terminal 10xHis tag. The purity of this MUC17 protein was greater than 95% by SDS-PAGE. The activity was tested.

As a newly discovered mucin, MUC17 (Mucin-17) belongs to the membrane-bound mucin family. Mucins are highly O-glycosylated linear glycoproteins secreted by higher organisms to protect and lubricate epithelial cell surfaces and are involved in the regulation of immune responses, inflammation, adhesion, and tumorigenesis. MUC17 is present in epithelial cells in specific human tissues. Its functions in epithelial cells are primarily to provide cytoprotection, maintain luminal architecture, signal transduction, and confer anti-adhesion properties to cancer cells that have lost apical/basolateral polarization. The biological function of MUC17 is thought to be to maintain the integrity of the mucosal barrier in the gut, such as mucosal restoration.
Datasheet & COA：

Please contact us to get it.

產品評價

平均分:

5.0分 - 1 個評價

實驗應用：抗原

產品評價：該產品經過ELISA實驗驗證具有很好的活性，后續用于動物免疫，包被抗原測試效價很高，篩選到了理想的雜交瘤陽性克隆。按照說明書正確保存，很長時間還可以用，性能穩定。整個實驗進展很順利！

By 杜老師

靶點詳情

功能：

Probably plays a role in maintaining homeostasis on mucosal surfaces.
基因功能參考文獻：
1. MUC17 polymorphisms are involved in endometriosis development and the associated infertility in the Taiwanese population. PMID: 26285705
2. Carbachol stimulated enterocyte MUC17 endocytosis is concomitant with NHE3 internalization and CFTR membrane recruitment. PMID: 23784542
3. The HIF1alpha-mediated hypoxic signal pathway contributes to MUC17 expression, and DNA methylation of hypoxia responsive element could be a determinant of the hypoxic inducibility of MUC17 in pancreatic cancer cells. PMID: 22970168
4. Both native and exogenous MUC17 play a role in attachment and invasion of enteroinvasive E. coli in colonic cell lines and in maintaining epithelial barrier function. PMID: 21393431
5. Hypomethylation status in the MUC17 promoter could be a novel epigenetic marker for the diagnosis of Pancreatic ductal adenocarcinomas. PMID: 20926598
6. Results indicate that the potential protective effects of this membrane-bound mucin are primarily or secondarily diminished in inflammatory and neoplastic conditions. PMID: 20702471
7. augments intestinal cell restitution and enhances healing of experimental colitis in mice PMID: 20211273
8. The authors demonstrate that apically atypical enteropathogenic Escherichia coli infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins. PMID: 20065027
9. sequence homology & chromosome mapping PMID: 11855812
10. surface localization of the smaller subunit of MUC17 is dependent on its N-glycosylation status PMID: 12888891
11. The MUC17 sequence has been extended toward its 5'-extremity to complete the sequence and localize the promoter and regulatory elements. PMID: 16737958
12. Pdzk1 plays a specific role in stabilizing Muc17 in the apical membrane of small intestinal enterocytes. PMID: 17990980
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亞細胞定位：

[Isoform 1]: Cell membrane; Single-pass membrane protein.; [Isoform 2]: Secreted. Cell membrane.
組織特異性：

Expressed almost exclusively in the intestine. Expression is especially high in both the duodenum and transverse colon. Expressed in mature absorptive cells of the small intestinal villi. No expression is detected in goblet cells. Highly expressed in panc
數據庫鏈接：

HGNC: 16800

OMIM: 608424

KEGG: hsa:140453

STRING: 9606.ENSP00000302716

UniGene: Hs.271819