Recombinant Mouse Protein Wnt-3a (Wnt3a)

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中文名稱：

小鼠Wnt3a重組蛋白
貨號：

CSB-EP026136MO
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Wnt3a
Uniprot No.：

P27467
別名：

Wnt3a; Wnt-3aProtein Wnt-3a
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

40.9 kDa
表達區域：

19-352aa
氨基酸序列

SYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHTCK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The N-terminal 10xHis tag was fused to the gene fragment corresponding to the 19-352aa of the mouse Wnt3a protein and then was cloned into an expression vector. The expression vector was transformed into the E.coli for expression. The generated product was purified and separated to obtain the recombinant mouse Wnt3a protein. Its purity is higher than 85%. It showed an apparent molecular weight of about 40-45 kDa under SDS-PAGE condition. This recombinant mouse Wnt3a protein may be used in the research about develpomental biology.
Wnt3a is a gene encoding a protein named Wnt3a and belongs Wnt family which consists of structurally related genes encoding secreted signaling proteins. These proteins are highly conserved across species. Wnt3a is ligand for members of the frizzled family of seven transmembrane receptors. It functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. WNT3A-associtaed diseases include Osteoporosis, Juvenile and Osteoporosis-Pseudoglioma Syndrome.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
基因功能參考文獻：
1. This work provides a new link between Wnt3a and macrophage-mediated angiogenesis under glucose and oxygen deprivation in vitro. PMID: 29199847
2. Lrp6 is the key mediator of Wnt3a signaling in osteoblasts and Lrp5 played a less significant role in mediating Wnt3a signaling. PMID: 29176883
3. These data define the mechanism responsible for the repressive effects of nitric oxide (NO) on the transcriptional activity of beta-catenin and link eNOS-derived NO to the modulation by VEGF of Wnt/beta-catenin-induced endothelial cell proliferation. PMID: 28320874
4. These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression PMID: 28093227
5. this study shows that the Wnt3a expression levels in dendritic cells influences the generation of memory T cells after 5 days in co-culture with naive T cells through activation of the Wnt canonical pathway PMID: 27544306
6. Wnt3a induces Osx expression via p38 MAPK signaling in dental follicle cells. Wnt3a-induced Osx expression was inhibited in the presence of p38 mitogen-activated protein kinase (MAPK) inhibitors (SB203580 and SB202190) at gene and protein levels, as assessed by real-time PCR and immunocytohistochemistry, respectively. PMID: 27450807
7. Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells PMID: 27633082
8. Wnt3a promotes macrophage-mediated bacterial killing by elevating CRAMP and BD1 levels. PMID: 26846714
9. Wnt3a acutely reduces nuclear acetyl-CoA, the necessary substrate for histone acetyltransferases, resulting in a global decrease in histone acetylation. PMID: 27129247
10. Wnt3a rapidly activated the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway and pharmacological stimulation of cAMP/PKA signaling suppressed osteoclast differentiation PMID: 26189772
11. Mesenchymal Stem Cells Within Gelatin/CaSO4 Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration. PMID: 26414873
12. Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus. PMID: 26956881
13. that activation of the Wnt pathway made cells more readily tolerate ROS-caused mitochondria injury and cell apoptosis PMID: 26085341
14. Pin1 is indispensable for Wnt3a-induced osteoblast differentiation. PMID: 26740630
15. The mouse xenograft model showed that Wnt3a-overexpressing cells grew into larger tumor masses and formed more vasculogenic mimicry than the control cells. PMID: 26266404
16. miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression. PMID: 26006230
17. Wnt8a and Wnt3a cooperate to maintain Fgf8 expression and prevent premature Sox2 up-regulation in the axial stem cell niche, critical for posterior growth. PMID: 25809880
18. WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo heterologous engraftment. PMID: 25084050
19. C1q-induced activation of Wnt3a/CTNNB1/FOXO1 signaling contributes to skeletal fiber type shift toward fatigable fiber in congestive heart failure. PMID: 26038536
20. Study reports that NRF2 participates in the formation of a protein complex with Axin1/GSK-3/beta-TrCP that is disrupted by WNT-3A, a prototypic canonical WNT ligand, and leads to upregulation of the NRF2 transcriptional signature. PMID: 25336178
21. Wnt3a and MMP9 induce C3H10T1/2 cells differentiation into cardiomyocyte-like cells. PMID: 25854560
22. Wnt3a/beta-catenin signaling directs trunk progenitors towards paraxial mesodermal progenitor fates; however, our data also suggest that Wnt3a positively supports a progenitor state for both mesodermal and neural progenitors. PMID: 25922526
23. the presented systems-biology approach demonstrates that Wnt3a attenuates RANKL-driven osteoclastogenesis by blocking c-Fos expression PMID: 25038457
24. Wnt3a/Frizzled-3 signaling plays important role in regulating the proliferation and differentiation of neural crest cells and various developmental stages of melanocyte precursors. PMID: 24815018
25. Sp5 and Sp8 are pivotal downstream effectors of Wnt3a/beta-catenin that are essential for the self-renewal and differentiation of neuromesodermal stem cells. PMID: 24475213
26. results suggest that Wnt3a overrides the effect of P2X7R on the Wnt/beta-catenin signaling to prevent the AEC I death and restrict the severity of ALI. PMID: 24922070
27. these data identify HES5 as a key mediator of the Wnt-3a proneurogenic effect occurring independently of the classical Wnt/beta-catenin signaling cascade thus further deciphering crosstalk mechanisms of Wnt and Notch signaling pathways regulating cell fate PMID: 24548083
28. These results indicate that JBP regulates the expressions of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice. PMID: 24841652
29. our results suggest a dose-dependent role for Wnt3a in regulating the balance between renewal and selection of differentiation fates of axial progenitors in the epiblast. PMID: 25152336
30. Mutation of any individual cysteine of Wnt3a results in covalent Wnt oligomers through ectopic intermolecular disulfide bond formation and diminishes/abolishes Wnt signaling. PMID: 24841207
31. epigenetic activation of Bmp2 gene expression contributes to Wnt3a-mediated direct trans-differentiation of pre-adipocytes or fibroblasts into osteoblasts PMID: 24867947
32. Increasing amounts of soluble Frizzled8-cysteine-rich-domain protein modulated Wnt3a signaling in a biphasic manner. PMID: 24885675
33. results demonstrate that, while ER signaling acts synergistically with Wnt3A in promoting osteogenic differentiation, Wnt3A may crosstalk with ER signaling by up-regulating ERalpha expression and down-regulating ERbeta expression in MPCs. PMID: 24340027
34. Wnt3a/beta-catenin signaling regulates follicular development by modulating the expression of Foxo3a signaling components. PMID: 24246780
35. Smad4 regulates maturation of skeletal collagen and osteoblast survival, and is required for matrix-forming responses to both BMP2 and Wnt3a. PMID: 24006258
36. Jianpi Bushen Prescription plays a protective role on radiation-induced bone marrow through the activation of the Wnt3a signaling pathway. PMID: 24114209
37. Structure-based mutational analysis of mouse Wnt3a shows that the linker between the N- and C-terminal domains is required for LRP6 binding. PMID: 23791946
38. Results demonstrated that Wnt3a regulates proliferation, apoptosis and enhances function of pancreatic NIT-1 beta cells via activation of Wnt/beta-catenin signaling. PMID: 23296977
39. Data indicate that Wnt/beta-catenin signaling mediates nandrolone-induced Numb transcription. PMID: 23649620
40. WNTs have a dual and context-dependent effect on microglia acting in a homeostatic pro- and anti-inflammatory manner. PMID: 23534675
41. We identified a number of stimuli triggering Wnt/beta-catenin pathway activation, including exogenous NKT cell activator, glycolipid alpha-GalCer, and endogenous prostaglandin E2 (PGE2). PMID: 22991247
42. Results support a potential mechanism for the differentiation of mesenchymal stem cells into type II alveolar cells involving canonical wnt pathway activation. PMID: 23154940
43. Wnt3a is a putative molecular partner of Zbtb20 in translating early positional information for the establishment of all developing medial pallidum fields. PMID: 22689450
44. findings suggest that a spatially restricted Wnt signal induces an oriented cell division in embryonic stem cells that generates distinct cell fates at predictable positions relative to the Wnt source PMID: 23520113
45. Wnt3a stimulates repaid translocation of IPMK to the cell membrane. PMID: 22940627
46. results identify LRP8 as a novel positive factor of canonical Wnt signaling pathway and show its involvement in Wnt-induced osteoblast differentiation PMID: 22589174
47. results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities PMID: 22761943
48. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. PMID: 22357953
49. Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. Wnt3a plays an important role in melanocyte homeostasis. PMID: 22710324
50. These data suggest that stabilization of beta-catenin by WNT3A inhibits BMP2-mediated induction of cementoblast/osteoblast differentiation in SVF4 cells, although BMP2 requires endogenous Wnt/beta-catenin signaling to promote cell maturation. PMID: 22150562
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亞細胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Wnt family
組織特異性：

Dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.
數據庫鏈接：

KEGG: mmu:22416

STRING: 10090.ENSMUSP00000010044

UniGene: Mm.1367