Recombinant Mouse Polyubiquitin-B (Ubb)

中文名稱：

小鼠Ubb重組蛋白
貨號(hào)：

CSB-YP025429MO
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Ubb重組蛋白
貨號(hào)：

CSB-EP025429MO
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Ubb重組蛋白
貨號(hào)：

CSB-EP025429MO-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ubb重組蛋白
貨號(hào)：

CSB-BP025429MO
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Ubb重組蛋白
貨號(hào)：

CSB-MP025429MO
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ubb
Uniprot No.：

P0CG49
別名：

Ubb; Polyubiquitin-B [Cleaved into: Ubiquitin]
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full length protein
表達(dá)區(qū)域：

1-76
氨基酸序列

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
基因功能參考文獻(xiàn)：
1. that UBB(+1) is specifically expressed in a subset of parasagittal stripes of Purkinje cells in the cerebellar cortex of a UBB(+1)-expressing mouse model PMID: 27966098
2. Data indicate that levels of repressor element-1 silencing transcription factor (REST) are elevated in polyubiquitin-B knockout (Ubb -/-) cells. PMID: 28285139
3. Ubb deficiency causes dysregulation of neural stem cell differentiation with premature gliogenesis. PMID: 25391618
4. study suggests that Ub homeostasis, or the maintenance of cellular free Ub above certain threshold levels, is essential for proper neuronal development and survival PMID: 25280998
5. The lysine 6 to tryptophan ubiquitin substitution results in the cataract development in newborn mice. PMID: 24450463
6. upon stress induced by disruption of Ubb, neuronal vulnerability may be determined based on the ability of neurons or neighboring cells to maintain free Ub levels for the protection of neuronal function and survival. PMID: 22285186
7. Several genes related to fertility, metabolism, transcription, and the ubiquitin-proteasome system (UPS) are misregulated in Ubb (-/-)testes at 7 and/or 14 days. In Ubb (-/-)testes ubiquitylated levels of histone H2A are significantly reduced. PMID: 21542049
8. Ubb knockout mice exhibit phenotypes consistent with hypothalamic dysfunction. Our data also indicate that Ubb is essential for the maintenance of the ubiquitin levels required for proper regulation of metabolic and sleep behaviours in mice. PMID: 20002312
9. Data show that ubiquitin B transgenic mice appear vulnerable to toxic insults due to a modest proteasome inhibition, and underscore the relevance of an efficient ubiquitin-proteasome system in Huntington disease. PMID: 20005957
10. targeted disruption of Ubb results in male and female infertility due to failure of germ cells to progress through meiosis I and hypogonadism. PMID: 18070917
11. Loss of Ubb led to a progressive degenerative disorder affecting neurons within the arcuate nucleus of the hypothalamus. PMID: 18299572
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亞細(xì)胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Ubiquitin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:22187

STRING: 10090.ENSMUSP00000019649

UniGene: Mm.371592