Recombinant Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (Ndufs4)

中文名稱：

小鼠Ndufs4重組蛋白
貨號：

CSB-YP880444MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Ndufs4重組蛋白
貨號：

CSB-EP880444MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Ndufs4重組蛋白
貨號：

CSB-EP880444MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ndufs4重組蛋白
貨號：

CSB-BP880444MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Ndufs4重組蛋白
貨號：

CSB-MP880444MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ndufs4
Uniprot No.：

Q9CXZ1
別名：

Ndufs4; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4; mitochondrial; Complex I-18 kDa; CI-18 kDa; Complex I-AQDQ; CI-AQDQ; NADH-ubiquinone oxidoreductase 18 kDa subunit
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
表達區域：

43-175
氨基酸序列

ADNQTRDT QLITVDEKLD ITTLTGVPEE HIKTRKVRIF VPARNNMQSG VNNTKKWKME FDTRERWENP LMGWASTADP LSNMVLTFSA KEDAIAFAEK NGWSYDVEEK KVPKPKSKSY GANFSWNKRT RVSTK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
基因功能參考文獻：
1. the partial absence of complex I sensitizes the myocardium towards ischemia reperfusion injury and that the main source of reactive oxygen species following reperfusion is complex III. PMID: 29501746
2. Excitatory synaptic transmission in the parietal association cortex in slices from Ndufs4(KO) animals was hypersensitive to isoflurane compared to control slices. We identified a direct neural circuit between the parietal association cortex and the central thalamus, consistent with a model in which isoflurane sensitivity is mediated by a thalamic signal relayed through excitatory synapses to the parietal association cort PMID: 29136012
3. Study showed that the earliest cell loss in complex 1-deficient Ndufs4 mice retinas is retinal bipolar cells at p20, followed by Starburst Amacrine Cells at p24, that precede a rise in inflammatory molecules at p30 and retinal ganglion cell death at p42; results could suggest a mechanism in which the death of bipolar and amacrine cells incite an inflammatory wave that ultimately results in retinal ganglion cell loss. PMID: 28027875
4. NDUFS4 deletion affected gene expression following neural differentiation and the potential of the cells to generate beating embryoid bodies. PMID: 26608563
5. Using the Ndufs4 knockout (Ndufs4 KO) mouse, a model of Leigh syndrome, we demonstrate for the first time that protein succination is increased in the brainstem (BS), particularly in the vestibular nucleus. PMID: 26450614
6. Locally insufficient respiration capacity of the nerve terminals may drive focal neurodegeneration in the Ndufs4 knockout mouse model of the Leigh syndrome. PMID: 26824698
7. This study demonstrated that Genetic reduction of Ndufs4 function does not lead to loss of dopamine neurons in vivo. PMID: 26070241
8. Reduced adolescent-age spatial learning ability is associated with elevated juvenile-age superoxide levels in complex I mouse mutants. PMID: 25853418
9. Mitochondrial complex I dysfunction in the retina, present in Ndufs4 KO mouse, triggers an innate immune and inflammatory response that results in loss of retinal ganglion cell function and death. PMID: 25652399
10. Global Ndufs4 deletion causes systemic inflammation and osteopetrosis. PMID: 25130399
11. Complex I deficiency due to selective loss of Ndufs4 in the mouse heart results in severe hypertrophic cardiomyopathy. PMID: 24705922
12. deletion of Ndufs4 results in a significant loss of complex I-supported respiration in the heart, which is well tolerated with no major changes of cardiac function, energetics, and longevity of the mice under unstressed conditions. PMID: 23931755
13. Oxidation of the reactive oxygen species sensor hydroethidium was increased and mitochondria were less branched and/or shorter in NDUFS4(-/-) fibroblasts. PMID: 23234723
14. the NDUFS4 subunit is of key importance in CI stabilization PMID: 22430089
15. results demonstrate that the loss of Ndufs4 causes only a mild complex I deficiency in vivo, which in dopamine neurons nevertheless leads to alterations that may play a causative or contributing role in the pathophysiology of late onset Parkinson's disease PMID: 22090423
16. Proteomic and metabolomic analyses of mitochondrial complex I-deficient mouse model generated by spontaneous B2 short interspersed nuclear element (SINE) insertion into NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) gene. PMID: 22535952
17. Tthe absence of NDUFS4 in different mouse tissues results in decreased activity and stability of mitochondrial complex I. PMID: 21965299
18. To explore the lethal, ataxic phenotype of complex I deficiency in Ndufs4 knockout mice, we inactivated Ndufs4 selectively in neurons and glia, resulting in progressive encephalopathy resembling Leigh syndrome. PMID: 20534480
19. CI fails to assemble properly or is unstable without NDUFS4 PMID: 18396137
20. In this study, Ndufs4-/- mice was used as a genetic model to study mitochondrial complex I deficiency and dopaminergic neuron death. PMID: 18812510
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亞細胞定位：

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
蛋白家族：

Complex I NDUFS4 subunit family
數據庫鏈接：

KEGG: mmu:17993

STRING: 10090.ENSMUSP00000022286

UniGene: Mm.253142