Recombinant Human Pancreatic adenocarcinoma up-regulated factor (ZG16B) (Active)

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中文名稱：

人ZG16B重組蛋白
品名簡稱：

Recombinant Human ZG16B protein (Active)
貨號：

CSB-MP836195HU
說明書：

MSDS Datasheet
規格：

￥828
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ZG16B at 2 μg/ml can bind Anti-ZG16B recombinant antibody (CSB-RA836195MA1HU), the EC₅₀ is 24.13-46.04 ng/mL. Biological Activity Assay

The purity of ZG16B was greater than 95% as determined by SEC-HPLC
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產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Human ZG16B at 2 μg/mL can bind Anti-ZG16B recombinant antibody (CSB-RA836195MA1HU), the EC₅₀ is 24.13-46.04 ng/mL.
基因名：

ZG16B
Uniprot No.：

Q96DA0
分子結構：
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

Mammalian cell
分子量：

20.0 kDa
表達區域：

53-208aa
氨基酸序列

GKMYGPGGGKYFSTTEDYDHEITGLRVSVGLLLVKSVQVKLGDSWDVKLGALGGNTQEVTLQPGEYITKVFVAFQAFLRGMVMYTSKDRYFYFGKLDGQISSAYPSQEGQVLVGIYGQYQLLGIKSIGFEWNYPLEEPTTEPPVNLTYSANSPVGR
蛋白標簽：

C-terminal 10xHis-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

This Human ZG16B recombinant protein was produced in mammalian cell, where the gene sequence encoding Human ZG16B (53-208aa) was expressed with the C-terminal 10xHis tag. The purity of this ZG16B protein was greater than 95% by SDS-PAGE. The activity was validated in functional ELISA.
ZG16B was first found to be highly expressed in pancreatic cancer and plays an important role in carcinogenesis, and was named as pancreatic cancer-related up-regulator PAUF. The latest research results show that ZG16B plays its role as an oncogene by up-regulating the expression and transcriptional activity of β-catenin in pancreatic cancer. At the same time, other studies have also found that ZG16B is not only highly expressed in pancreatic cancer, but also in colon cancer. It is also highly expressed in ovarian cancer, gastric cancer and other cancer tissues. Furthermore, ZG16B plays an important role in cancer progression such as cell proliferation, adhesion, migration, and invasion. However, in colon cancer, whether ZG16B plays a role in cancer by upregulating the expression and transcriptional activity of β-catenin and activating the Wnt/β-catenin signaling pathway is still unknown. As a novel activator of human endothelial cells, ZG16B has similar effects to vascular endothelial growth factor (VEGF), which can promote angiogenesis and increase vascular permeability in vitro and in vivo.
Datasheet & COA：

Please contact us to get it.

產品評價

平均分:

5.0分 - 1 個評價

實驗應用：ELISA標準品

產品評價：我們包被human ZG16B做ELISA測試我們的抗體，EC50為24.33-45.30 ng/ml，通過ELISA和流式進行抗體篩選，結果很順利，蛋白質量挺好的，活性好，穩定！

By 王老師

靶點詳情

基因功能參考文獻：
1. we conclude that the PAUF is a powerful and multifunctional promoter of tumor growth through increase and functional activation of myeloid-derived suppressor cells PMID: 27322081
2. Mouse DCPP1 is a multifunctional promoter of tumor growth through functional activation of pancreatic cancer cells, suggesting it to be an ortholog of human PAUF. PMID: 28988106
3. Our results suggest that PAUF has tumour-promoting functions in oral squamous cell carcinoma PMID: 27706833
4. High PAUF expression is associated with resistance to gemcitabine in pancreatic cancer. PMID: 26684804
5. PAUF mediated dendritic cell activation and immune stimulation are dependent on TLR4 PMID: 26336989
6. constitutive suppression of PAUF sensitized Bxpc3 pancreatic cancer cells to oncolytic parvovirus H-1 infection PMID: 25727013
7. PAUF rs12373A>C polymorphisms are associated with colorectal cancer. PMID: 25079514
8. PAUF-siRNA inhibited the proliferation of colorectal cancer cells, promoted their apoptosis and induced G0/G1 cell cycle arrest. At the same time, PAUF-siRNA inhibited the invasion, adhesion and migration of the tumor cells. PMID: 23677445
9. new possibilities for PAUF's role in the pathogenesis of angiogenesis-dependent diseases PMID: 22907431
10. activation of SIRT1 inhibited the proliferation of pancreatic cancer -PAUF cells by down-regulation of cyclin-D1, a target molecule of beta-catenin. PMID: 22640743
11. PAUF-mediated FAK activation plays an important role in pancreatic cancer progression. PMID: 21464589
12. PAUF can up-regulate and stabilize beta-catenin via a novel pattern of phosphorylation, thereby contributing to the rapid proliferation of pancreatic cancer cells. PMID: 21196815
13. Data demonstrate that the host salivary protein CSP-1 (HRPE773 GenBank AAQ89380.1) binds to S. mutans cells and may influence the initial colonization of this pathogenic bacterium onto the tooth surface. PMID: 20858015
14. the sugar-binding site and the adjacent basic patch of ZG16p and ZG16b cooperatively form a functional glycosaminoglycan-binding site. PMID: 21110947
15. PAUF is a mammalian lectin normally found in plant lectins. PAUF induces extracellular signal-regulated kinase phosphorylation and activates the IKK-b-mediated TPL2/MEK/ERK signaling pathway through TLR2. PMID: 20802527
16. Findings indicate that PAUF enhances the metastatic potential of pancreatic cancer cells, at least in part, by upregulating CXCR4 expression. PMID: 19784070
17. Bioinformatic analysis identified a putative human CSP-1/Dcpp ortholog, HRPE773, expressed predominantly in human salivary tissue, that shows 31% amino acid identity and 45% amino acid similarity to the mouse Dcpp query sequence. PMID: 16954406
18. pancreatic adenocarcinoma up-regulated factor was secreted into the culture medium of pancreatic adenocarcinoma up-regulated factor-overexpressing Chinese hamster ovary cells, had an apparent molecular mass of approximately 25 kDa, and was N-glycosylated PMID: 19302292
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亞細胞定位：

Secreted.
蛋白家族：

Jacalin lectin family
數據庫鏈接：

HGNC: 30456

KEGG: hsa:124220

STRING: 9606.ENSP00000371715

UniGene: Hs.105887