E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized
powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
In early stages, overexpression of Sca-1 decreases tumour formation while at later stages overexpression of Sca-1 seems to drive tumours towards malignant progression. PMID: 28732240
Sca-1 is a novel cancer stem cells enrichment marker. PMID: 26869189
IL-27-induced Sca-1(+) T cells had increased expression of effector/memory-associated transcription factor T-bet, Eomes and Blimp1. PMID: 28758191
SCA-1 enriches for an ERalpha(+), estrogen-sensitive subpopulation within the CD24(+)/CD49f(hi) MaSC population that may be responsible for the hormonal sensitivity of the developing mammary gland. PMID: 28132885
Upregulation of leptin levels in both the vessel wall and the circulation after vessel injury promoted the migration of Sca-1(+) progenitor cells via leptin receptor-dependent signal transducer and activator of transcription 3- Rac1/Cdc42-ERK (extracellular signal-regulated kinase)-FAK pathways, which enhanced neointimal formation. PMID: 28935755
Both the number of lung CD31-CD45-Sca-1+ cells and the expression levels of the Shh signaling pathway were downregulated in the lung tissues of mice with pulmonary emphysema. These cells and Shh signaling pathway are reactivated during acute adenovirus infection. PMID: 28352167
we report significantly higher expression of the immunoglobulin light chain by B cells in lamina propria of Ly-6A/Sca-1deficient mice when compared to the wild-type control. PMID: 27322740
c-Myb regulates proliferation/differentiation of adventitial Sca1+ vascular smooth muscle progenitor cells by transcriptional activation of myocardin. PMID: 27174098
sca-1 antibody reduces both CD34+/c-kit+ progenitor cell surge and vascular restenosis after endoluminal vascular injury in a murine model. PMID: 27666446
Results indicate that IL-17A promotes tumorigenicity of colon cancer cells by enhancing cell cycle progression and directly induces the expression of Sca-1. PMID: 27378226
Study provides evidence that up-regulation of miRNA-21 can promote migration and proliferation of Sca-1+ CSCs to enhance the capacity of Sca-1+ CSCs to repair damaged myocardium. PMID: 27210794
the androgen-independent and bipotent organoid-forming Sca-1(+) luminal cells as a functionally distinct cellular entity, is reported. PMID: 26418304
Sphingosylphosphorylcholine promotes the differentiation of resident Sca-1 positive cardiac stem cells to cardiomyocytes through lipid raft/JNK/STAT3 and beta-catenin signaling pathways. PMID: 27066979
Data show that bisperoxovanadium (BpV) treatment induces specific epigenetic modifications at the promoter regions of genes associated with stem cell fate, including Sca-1 and Pw1. PMID: 26672000
The results suggested that the depletion of the progenitor cell pool and DNA methylation of Sca-1 gene may be involved in the progression of emphysema in mice. PMID: 26264445
PDGFRalpha has a role in demarcating the cardiogenic clonogenic Sca1+ stem/progenitor cell in adult murine myocardium PMID: 25980517
The progenitor phenotype of the Sp-C(+)Sca-1(+) cells that mediates alveolar epithelial repair might involve Wnt signaling. PMID: 25474582
Subcutaneous and visceral fat-derived Sca1(high) ASCs particularly differ in the gene expressions of adhesion and ECM molecules. PMID: 24726953
The more aggressive behavior of Ly6a/Sca-1 expressing leukemias is due at least in part to greater capacity to degrade microenvironmental stroma and invade tissues. PMID: 24586463
Reversal of ischemic cardiomyopathy with Sca-1+ stem cells modified with multiple growth factors. PMID: 24705272
Sca1 and bromodeoxyuridine positive cells may participate in the formation of new thyroid follicles after partial thyroidectomy. PMID: 24278321
The high and specific expression of stem cell antigen 1 (Sca1) in TtT/GF cells was confirmed by real-time polymerase chain reaction. PMID: 23881407
Application of hematopoietic progenitor (lin-/sca-1+) or endothelial progenitor cells potentially improves the inflammatory and oxidative stress parameters in an atherosclerosis model. PMID: 22622053
the A2B receptor signaling linked to up-regulation of pro-angiogenic factors in cardiac Sca-1(+)CD31(-) stromal cells is essential for overall improvement of cardiac recovery seen after their transplantation to the injured heart. PMID: 23827818
Report the distribution of c-kit/Sca-1/CXCR4 expressing cardiac stem cells in the different compartments of the heart and significant reduction in their number in adult heart. PMID: 23273785
Sca-1(-) Plasmacytoid dendritic cells are an early developmental stage of Plasmacytoid dendritic cells with distinct innate functions representing the true murine natural IFN-alpha-producing cells. PMID: 23922217
These results demonstrate that TLR4/Sca-1 signaling plays an important role in the regulation of hematopoietic precursor cell programming and their enhancement of granulocyte lineage commitment in response to E. coli bacteremia. PMID: 23545304
PECAM1(+)/Sca1(+)/CD38(+) vascular cells could proliferate and differentiate into myofibroblast-like cells in wound repair. PMID: 23308177
Sca-1(+) cells expressed markedly higher levels of mammary stem cell-related genes in comparison to Sca-1(-) cells. PMID: 22634533
Identification of a population of LY6A+ mouse ovarian surface epithelium progenitor cells on the surface of the ovary that may play a role in ovulatory wound healing. PMID: 22914315
results of this study suggest that increased Sca1 expression on erythropoietic precursors inhibits erythroid differentiation PMID: 23246681
Sca-1 protects against cardiac hypertrophy and fibrosis via regulation of multiple pathways in cardiomyocytes. PMID: 22851736
Data show that deletion of Sca-1 causes primary cardiac defects in myocardial contractility and repair consistent with impairment of resident cardiac progenitor cell proliferative capacity. PMID: 22800687
Sca-1 is either redundant or a nonessential marker of adipose progenitor/stem cells. PMID: 21510817
Studies suggest that the cloned Sca-1+CD45- cells confer the therapeutic benefits of Cardiospheres (CSs) in the mouse myocardial infarction (MI) model. PMID: 22272337
Sca-1 is a negative regulator of the tumor suppressor effects of PPARgamma PMID: 21955520
enhanced Sca-1 expression facilitates activation of the ERK pathway and supports the proliferation of myeloid and granulopoietic precursors during bacteremia; alcohol intoxication suppresses this response PMID: 22238460
MicroRNA-126 modulates endothelial SDF-1 expression and mobilization of Sca-1(+)/Lin(-) progenitor cells in ischaemia. PMID: 21856785
These data demonstrate the crucial role of Sca-1 in the maintenance of cardiac integrity and suggest that Sca-1 restrains spontaneous differentiation in the precursor population. PMID: 21957128
Results imply that Sca-1-positive cells may have a role in the duct cell proliferation in the regeneration step elicited by excretory duct ligation-induced injury. PMID: 21884259
Postulate that SCA1(+)/CD31(+) cardiac side population cells may represent endothelial progenitor cells in the mouse heart. PMID: 21615679
Hematopoietic lineage cells with Sca-1(high) expression and CD62L(neg/low) expression are characterized as multipotent progenitor cells, based on transient engraftment. PMID: 21998453
Ly6a as a candidate gene for functional involvement in novelty responsiveness PMID: 21673958
Inhalation of acrolein increases apoptosis and suppresses the circulating levels of Flk-1(+)/Sca-1(+) cells while increasing these cells in the bone marrow and preventing their mobilization by VEGF. PMID: 21527748
Sca-1 attenuated GDF10-dependent TGF-beta signaling by disrupting the heterodimerization of TbetaRI and TbetaRII receptors. PMID: 21518866
TGF-beta1 represses Sca-1 expression in T cells and other immune cell populations derived from the spleen, indicating that regulation by TGF-beta1 is a general feature of Sca-1 expression in multiple cell types PMID: 21156809
Sca-1-/- mice have a defect in their capacity to recruit soluble IgM, and subsequently C3 complement, to damaged muscle. There was a significant reduction in B-1a cells in Sca-1-/- mice. PMID: 21123737
Prior exposure of mesenchymal stem cells to hypoxia led to a significant reduction of ex vivo expansion time, with significantly increased numbers of Sca-1(+) as well as Sca-1(+)/CD44(+)double-positive cells. PMID: 20496083
Ly-6C(hi) monocytosis disturbs resolution of inflammation in murine infarcts and consequently enhances left ventricular remodeling. PMID: 20378083