Recombinant Mouse Crossover junction endonuclease EME1 (Eme1)

中文名稱：

小鼠Eme1重組蛋白
貨號：

CSB-YP806857MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Eme1重組蛋白
貨號：

CSB-EP806857MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Eme1重組蛋白
貨號：

CSB-EP806857MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Eme1重組蛋白
貨號：

CSB-BP806857MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Eme1重組蛋白
貨號：

CSB-MP806857MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Eme1
Uniprot No.：

Q8BJW7
別名：

Eme1Crossover junction endonuclease EME1; EC 3.1.22.-
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-570
氨基酸序列

MALRRLSLSR LSTESDSEDL PTFAFLKKEP SSTNRKPPQR AKNIVVVTSD SEASCPPSPG LKGPPCVPSA AGAPPQAGPV RVLSSSSEDE DVFVPLAERI TCKLLTSKQL CPELSSSSLK TGLDGQNNAS APCDWKRQPW PKIPDVPLHG ALEKSAANDE DSLLDDQCRQ LPTYQATCRE LAVSKTNSDR PLPKKRTKHI QTVQSGGSQG CWRPGQASRK ENTPRQHERK KKAEMIKRLK AQRPEECLKH IVVVLDPVLL QMEGGGQLLG ALQAMECSCV IEVQAIPRSI TWRRRRTELV EDGDDWMEEP TILVLVLAEV FMSMAYNLKQ ASPSSTEKGK ETLRSFVTDV TAKTGKALSL VIVDQEKCFR PQNPPRRRKS GMANKQAKAK HQQRQESSTG LMVSRADMEK ALVDLQLYTE AQAWMVQSWK ELADFTCAFT KAVAEAPFKK LRDQVTFSFF LEKDWAGGMK VDQSGRGLAL IWRRQIQQLN RVSSEMASAI VDAYPSPQLL VQAYQRCFSE QERQNLLADI QVRRGEGVTA TSRRVGPELS RRIYLQMTTA QPDLILDSVD
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
基因功能參考文獻：
1. indicate that SLX1 and MUS81-EME1 nucleases act together to resolve Holliday junctions (HJs) in a manner that requires tethering to SLX4. PMID: 24076219
2. These findings link Eme1 with the replication-associated chromatin modifier functions of Np95 in the cellular response to DNA damage. PMID: 18692478
亞細胞定位：

Nucleus, nucleolus.
蛋白家族：

EME1/MMS4 family
組織特異性：

Weakly expressed in brain, heart, kidney, liver, lung, muscle, skin, small intestine, spleen, stomach, testis and thymus. Expressed in bone marrow. Also expressed in embryonic stem cells (ES cells).
數據庫鏈接：

KEGG: mmu:268465

STRING: 10090.ENSMUSP00000036361

UniGene: Mm.358937