Recombinant Human Remodeling and spacing factor 1 (RSF1), partial

中文名稱：

Recombinant Human Remodeling and spacing factor 1 (RSF1), partial
品名簡稱：

Recombinant Human RSF1 protein, partial
貨號：

CSB-EP020540HU
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

RSF1
Uniprot No.：

Q96T23
別名：

HBV pX-associated protein 8;Hepatitis B virus X-associated protein;p325 subunit of RSF chromatin-remodeling complex
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

17.4 kDa
表達(dá)區(qū)域：

1343-1441aa
氨基酸序列

IESDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPTEKSQTPKDNSTASASLASNGTSGGQEAGAPEEEEDELLRVTDLVDYVCNSEQL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Regulatory subunit of the ATP-dependent RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. Binds to core histones together with SMARCA5, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex. Directly stimulates the ATPase activity of SMARCA1 and SMARCA5 in the RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, respectively. The RSF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the RSF-5 ISWI chromatin-remodeling complex. The complexes do not have the ability to slide mononucleosomes to the center of a DNA template. Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain.
基因功能參考文獻(xiàn)：
1. Remodeling and spacing factor 1 (RSF1) stability is regulated at the post-translational level upon DNA damage. PMID: 29385673
2. Results show that RSF1 is highly expressed in bladder cancer tissues (BC) and is negatively correlated with miR-154 levels. RSF1 is a direct target by miR-154 in BC cells. There was however no significant effect on RSF1 mRNA levels, which may be due to incomplete complementation with the 3'-UTR. PMID: 29048677
3. In osteosarcoma patients, RSF1 was increased and associated with advanced clinical features and poor overall survival. PMID: 28843909
4. these data identify RSF1 as a H2Aub reader that contributes to H2Aub-mediated gene silencing by maintaining a stable nucleosome pattern at promoter regions. PMID: 28855339
5. Rsf-1 was highly expressed in H460 and H1299 cells. Rsf-1 knockout caused cell arrest at the G1 phase, increased cell apoptosis, and decreased migration and cell proliferation. Rsf-1 knockout increased the inhibition of cell proliferation, the reduction in cell migration and the augment in cell apoptosis in paclitaxel treated H460 and H1299 cells. PMID: 29258089
6. Rsf-1 was overexpressed in lung cancer and associated with poor survival. Rsf-1 regulated cell invasion through MMP2 and NF-kappaB pathway. PMID: 28289901
7. essential centromeric component that recruits PLK1 to kinetochores and plays a crucial role in faithful cell division PMID: 26259146
8. High-expression of Rsf-1 is associated with pathologic subtypes of breast cancer, aggressive phenotype, p53 positive and poor clinical outcome, which confers tumor aggressiveness through chromatin remodeling. PMID: 25337201
9. RSF1 facilitates DNA double-strand break repair by recruiting centromeric and Fanconi Anaemia proteins. PMID: 24800743
10. Chromatin remodeler Rsf-1 is a guard in DNA damage checkpoints and homologous recombination repair. PMID: 24351651
11. RSF1 plays a critical role in DNA double stranded break repair, a process in which it acts independently from its binding partner SMARCA5. PMID: 25111480
12. Rsf-1 contributes to hepatocellular carcinoma cell growth through regulation of cell cycle proteins. PMID: 24798976
13. Rsf-1 contributes to prostate cancer cell growth and invasion, which makes it a candidate therapeutic target. PMID: 24584698
14. Remodeling and spacing factor 1 promotes the assembly of CENP-S and CENP-X at damaged chromatin and regulates DSB repair by homologous recombination and non-homologous end-joining. PMID: 23974106
15. Serum HBXAP DNA from 65 lung cancer patients and 20 healthy controls was quantified using real-time fluorescent quantitative polymerase chain reaction. PMID: 23456648
16. Rsf-1 increases the frequency of abnormal mitotic events by disrupting hBubR1-Cdc20 interactions. PMID: 23536579
17. Rsf-1 interacts and collaborates with cyclin E1 in neoplastic transformation and TP53 mutations are a prerequisite for tumour-promoting functions of the RSF/cyclin E1 complex. PMID: 23378270
18. High RSF-1 expression correlates with poor prognosis in patients with gastric adenocarcinoma. PMID: 22977663
19. Rsf-1 is overexpressed in colon cancers and contributes to malignant cell growth by cyclin E and phospho-Rb modulation, which makes Rsf-1 a candidate therapeutic target in colon cancer. PMID: 22528946
20. Overexpression of Rsf-1 is associated with higher tumour stage and poorer clinical outcome in urinary bladder urothelial carcinoma. PMID: 22685262
21. High-expression of Rsf-1 is associated with poor therapeutic response and adverse outcome in rectal cancer patients treated with neoadjuvant chemoradiotherapy. PMID: 22569540
22. Rsf-1 is overexpressed in non-small cell lung cancers and contributes to malignant cell growth by cyclin D1 and ERK modulation. PMID: 22387541
23. Rsf-1 overexpression is common and is associated with adverse prognosticators and therapeutic response in nasopharyngeal carcinoma. PMID: 22081787
24. Rsf-1 overexpression is common and associated with adverse prognosticators in gallbladder carcinomas. PMID: 21995635
25. our results suggest that RSF-1 up-regulation is associated with several clinicopathological features of disease aggressiveness in oral squamous cell carcinoma PMID: 21514451
26. a higher expression level of Rsf-1 is associated with advanced clinical stage and lymph node metastasis in ovarian clear-cell carcinoma. PMID: 21131837
27. Rsf-1, a chromatin remodeling protein, induces DNA damage and promotes genomic instability PMID: 20923775
28. Suggest that overexpression of Rsf-1, cyclin E and FASN occurs early in ovarian tumor progression. PMID: 20228782
29. HBXAP represses NF-kappaB-mediated gene activation in a dose-dependent manner. Our results showed that HBXAP and NF-kappaB colocalize to the nuclear matrix with specific physical interaction between them. PMID: 15242768
30. An important role of Rsf-1 amplification in ovarian cancer was shown. PMID: 16172393
31. Putative oncogene in ovarian cancer. PMID: 16418735
32. Rsf-1 expression is down-regulated in breast carcinoma cells in effusions compared with the solid counterparts and has no prognostic role at this anatomic site. PMID: 18289639
33. In an ovarian carcinoma cohort, RSF1 was amplified in 12% of the cases. It was correlated with serous histology and a worse prognosis. The 11q13 amplicon in ovarian cancer is likely driven by a cassette of genes rather than by a single oncogene. PMID: 18314909
34. findings suggest that interaction between Rsf-1 and hSNF2H may define a survival signal in those tumors overexpressing Rsf-1 PMID: 18519663
35. Rsf-1 is the major gene within the 11q13.5 amplicon that contributes to paclitaxel resistance in ovarian cancers. PMID: 19190325
36. continued expression of the ATX transgenes can contribute to hepatocyte transformation but are not sufficient to trigger neoplastic changes in the liver PMID: 19475691
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亞細(xì)胞定位：

Nucleus.
組織特異性：

Ubiquitously expressed. Highly expressed in the heart, skeletal muscle, kidney and placenta. Expressed at low levels in the brain and colon.
數(shù)據(jù)庫鏈接：

HGNC: 18118

OMIM: 608522

KEGG: hsa:51773

STRING: 9606.ENSP00000311513

UniGene: Hs.420229