Recombinant Human RWD domain-containing protein 3 (RWDD3 RSUME)

中文名稱：
貨號：

CSB-YP168100HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：
貨號：

CSB-EP168100HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：
貨號：

CSB-EP168100HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：
貨號：

CSB-BP168100HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：
貨號：

CSB-MP168100HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RWDD3
Uniprot No.：

Q9Y3V2
別名：

RWD domain-containing protein 3; RWD domain-containing sumoylation enhancer; RSUME; RWDD3 RSUME
種屬：

Homo sapiens (Human)
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination.
基因功能參考文獻：
1. miR-375 was downregulated in human gliomas and overexpression of miR-375 caused downregulation of RWDD3 in glioma cells as well as inhibited their motility. Thus, these findings suggest that RWDD3 and miR-375 may function as therapeutic biomarkers for glioma patients PMID: 29436665
2. RSUME has a role in tumorigenesis and metastasis of pancreatic neuroendocrine tumors PMID: 27506944
3. RSUME affects post-transcriptional expression of HIF-1a by regulating VEGF-A secretion and pituitary adenoma cells invasiveness. PMID: 27989771
4. the present report suggest that the association of RWDD3 and TECTA with paclitaxel-induced peripheral neuropathy may have been a false positive signal PMID: 25549536
5. an important role of RSUME in cancer and inflammation PMID: 26297826
6. RSUME is expressed in VHL tumors and inhibits VHL and regulates its tumor suppressor function. PMID: 25500545
7. this study failed to replicate a GWAS reporting an association between the 2 SNPs rs2296308 in RWDD3 and rs1829 in the intron of TECTA and time to neuropathy in ovarian cancer patients treated with paclitaxel PMID: 22877241
8. a degree of redundancy of the RSUME variants on the SUMO pathway PMID: 23469069
9. RSUME interacts with glucocorticoid receptor and increases its SUMOylation and transcriptional activity, pointing to SUMO conjugation as the cause for transcriptional stimulation. PMID: 23508108
10. RSUME critically regulates HIF-1a and VEGF-A production and thus might play an important role in pituitary tumour neovascularisation PMID: 22009797
11. RSUME plays a central role in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells [RSUME]. PMID: 17956732
12. BMP-4 and RSUME may be interesting targets for inhibiting steps involved in pituitary tumorigenesis. PMID: 19407504
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亞細胞定位：

Nucleus. Cytoplasm.
組織特異性：

Isoform 1 and isoform 2 are expressed in glioma tumors (at protein level). Expressed in a wide number of tissues with highest expression in cerebellum, pituitary, heart, kidney, liver, stomach, pancreas, prostate and spleen. Low levels in thalamus, spinal
數據庫鏈接：

HGNC: 21393

OMIM: 615875

KEGG: hsa:25950

STRING: 9606.ENSP00000359221

UniGene: Hs.483512