Recombinant Human Nuclear receptor corepressor 1 (NCOR1), partial

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中文名稱：

人NCOR1重組蛋白
品名簡稱：

Recombinant Human NCOR1 protein, partial
貨號：

CSB-EP015547HU
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

NCOR1
Uniprot No.：

O75376
別名：

N-CoR;N-CoR1
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

48.3 kDa
表達區域：

1-373aa
氨基酸序列

MSSSGYPPNQGAFSTEQSRYPPHSVQYTFPNTRHQQEFAVPDYRSSHLEVSQASQLLQQQQQQQLRRRPSLLSEFHPGSDRPQERRTSYEPFHPGPSPVDHDSLESKRPRLEQVSDSHFQRVSAAVLPLVHPLPEGLRASADAKKDPAFGGKHEAPSSPISGQPCGDDQNASPSKLSKEELIQSMDRVDREIAKVEQQILKLKKKQQQLEEEAAKPPEPEKPVSPPPVEQKHRSIVQIIYDENRKKAEEAHKIFEGLGPKVELPLYNQPSDTKVYHENIKTNQVMRKKLILFFKRRNHARKQREQKICQRYDQLMEAWEKKVDRIENNPRRKAKESKTREYYEKQFPEIRKQREQQERFQRVGQRGAGLSATI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Mediates the NR1D1-dependent repression and circadian regulation of TSHB expression. The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene ARTNL/BMAL1 and the genes involved in lipid metabolism in the liver.
基因功能參考文獻：
1. verexpression of COPS5, through its isopeptidase activity, leads to ubiquitination and proteasome-mediated degradation of NCoR, a key corepressor for ERalpha and tamoxifen-mediated suppression of ERalpha target genes. PMID: 27375289
2. Authors previously shown that Nuclear Receptor Corepressor 1 (NCoR) and the thyroid hormone receptor beta1 (TRbeta) inhibit tumor invasion. Here they show that these molecules repress VEGF-C and VEGF-D gene transcription in breast cancer cells, reducing lymphatic vessel density and sentinel lymph node invasion in tumor xenografts. PMID: 27806339
3. Nuclear Receptor Corepressor 1 is an important transcriptional regulator that interacts with nuclear receptors and other transcription factors. Recent results have shown the presence of inactivating mutations or deletions of the nuclear receptor corepressor 1 gene in human tumors. PMID: 27149915
4. NCOR1 function declines with prostate cancer progression. Reduction in NCOR1 levels causes bicalutamide resistance in LNCaP cells and compromises response to bicalutamide in mouse prostate in vivo PMID: 26968201
5. USP44 contributes to N-CoR functions in regulating gene expression and is required for efficient invasiveness of triple-negative breast cancer cells. PMID: 27880911
6. PDCD2 and NCoR1 may act as tumor suppressors in Gastrointestinal stromal tumors cells through the Smad signaling pathway. PMID: 26589942
7. NCoR depletion enhances cancer cell invasion and increases tumor growth and metastatic potential. PMID: 26729869
8. loss of nuclear NCoR results in upregulation of a specific cancer-related genetic signature, and is significantly associated with malignant melanoma progression. PMID: 25823659
9. The co-localization of AML1-ETO with the N-CoR co-repressor to be primarily on genomic regions distal to transcriptional start sites. (NcoR1) PMID: 25928846
10. Data suggest that direct interactions of HLCS (holocarboxylase synthetase) with NCOR1 (nuclear receptor corepressor 1) and HDAC1 (histone deacetylase 1) contribute toward transcriptional repression of repeats, presumably increasing genome stability. PMID: 24840043
11. Low NCoR expression is associated with glioblastoma. PMID: 24335696
12. Site directed mutagenic analysis of N-CoR identified serine 1450 as the crucial residue whose phosphorylation by Akt was essential for the misfolding and loss of N-CoR protein. PMID: 23940660
13. Study shows that NCoR1 is a key target of proteolysis and physically interacts with the transcription factor CREB, the genome-wide map described here ties proteolysis in mammalian cells to active enhancers and to promoters of specific gene families. PMID: 24315104
14. The aberrant cytoplasmic expression of NCoR1 in retinoblastoma appears to be associated with the proliferative and/or dedifferentiated properties of retinoblastoma. PMID: 23295231
15. Corepressor molecules NCoR and SMRT are present at 1,25(OH)2D3 activated gene enhancers PMID: 22944139
16. NCOR1 and HDAC3 are instrumental in the repression of glucocorticoid receptor gene transcription. PMID: 23428870
17. These results uncover a regulatory mechanism by which PKA positively modulates NCoR function in transcriptional regulation in prostate cancer. PMID: 23129261
18. CK2alpha-NCoR cascade selectively represses the transcription of IP-10 and promotes oncogenic signaling in human esophageal cancer cells. PMID: 22675025
19. A novel mechanism by which overexpression of estrogen receptor (ER) beta through NCoR is able to down regulate ER alpha gene expression, thus blocking ER alpha's driving role on breast cancer cell growth. PMID: 22622808
20. Findings suggest that N-CoR-induced repression of Flt3 might be crucial for limiting the contribution of the Flt3 signaling pathway on the growth potential of leukemic cells. PMID: 22514634
21. Regulated HDAC3 degradation serves as a buffering mechanism to protect independent formation of N-CoR and SMRT corepressor complexes. PMID: 22337871
22. Data suggest a possible role of misfolded N-CoR protein in the activation of oncogenic survival pathway in non-small cell lung cancer cells. PMID: 21966475
23. ERbeta and its co-regulators p300 and NCoR are expressed in human transitional cell bladder cancer PMID: 21525722
24. the aberrant recruitment of NCOR1 by TRbeta mutants leads to clinical resistance to thyroid hormone (RTH) PMID: 21987803
25. Differential interaction of NCoR1 with TR isoforms accounted for the TR isoformdependent regulation of adipogenesis and that aberrant interaction of NCoR1 with TR could underlie the pathogenesis of lipid disorders in hypothyroidism. PMID: 21389087
26. data strongly support a model in which EBNA2 association with NCoR-deficient RBPJ enhances transcription and EBNALP dismisses NCoR and RBPJ repressive complexes from enhancers PMID: 21518914
27. These data support the hypothesis that NCoR might control a cell cycle dependent regulation of expression androgen receptor target genes in prostate cells. PMID: 20974212
28. Aberrant corepressor interactions implicated in PML-RAR(alpha) and PLZF-RAR(alpha) leukemogenesis reflect an altered recruitment and release of specific NCoR and SMRT splice variants. PMID: 21131350
29. Amino-terminal A/B domain deletion facilitated the in vitro binding of nuclear receptor CoR with wild-type PPARG2. PMID: 20587609
30. Elevated NCOR1 disrupts PPARalpha/gamma signaling and is associated with prostate cancer. PMID: 20466759
31. This paper describes the cloning of the full-length human NCOR1 cDNA. PMID: 9724795
32. The authors established an interaction of E8;E2C with an NCoR1/HDAC3 complex and demonstrated that this interaction requires the wild-type E8 open reading frame. PMID: 20181716
33. NCOR1 protein expression level predicts response to endocrine therapy as first-line treatment for breast cancer patients on relapse. PMID: 19781322
34. Nuclear receptor corepressor-dependent repression of peroxisome-proliferator-activated receptor delta-mediated transactivation PMID: 11903058
35. These results demonstrate that AR, in contrast to other SHRs, is regulated by NCoR PMID: 12089345
36. Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappaB and beta-amyloid precursor protein. PMID: 12150997
37. recruited by prohibitin for transcriptional repression PMID: 12466959
38. N-CoR functions not merely as a repressor of basal transcription, but rather as a modulator of both basal and ligand-activated transcription of genes controlled by RAR/RXR heterodimers in a dose-dependent manner PMID: 12648520
39. associates with CHD1 and histone deacetylase as well as with RNA splicing proteins PMID: 12890497
40. N-CoR utilizes repression domains I and III for interaction and co-repression with ETO PMID: 15377655
41. NCoR is a physiological regulator of the AR; the N-terminal surface of the AR-mediating NCoR recruitment was distinct from tau5 and from the FXXLF motif that mediates agonist-induced N-C-terminal interaction PMID: 15598662
42. the DAD domain of N-CoR is singularly essential for repression by the thyroid hormone receptor PMID: 15695367
43. N-CoR and SMRT play an active role in preventing tamoxifen from stimulating proliferation in breast cancer cells through repression of a subset of target genes involved in ERalpha function and cell proliferation PMID: 15802375
44. N-CoR together with JMJD2A could play a role in repressing achaete scute-like homologue 2 (ASCL2) expression in various tissues. PMID: 16024779
45. mechanism by which the estrogen-ER complex markedly reduces the level of N-CoR through a process involving the up-regulation of Siah2 and the subsequent targeting of N-CoR for proteasomal degradation PMID: 16141343
46. SAFB1 was shown to interact directly with the nuclear receptor corepressor N-CoR. PMID: 16195251
47. N-CoR and TRbeta cooperate in the regulation of the TSHbeta gene and this ligand-dependent repression is mediated by the LXXLL motif in N-CoR PMID: 16216492
48. SMRT and N-CoR corepressors are involved in transcriptional regulation by both agonist- and antagonist-bound AR and regulate the magnitude of hormone response, at least in part, by competing with coactivators. PMID: 16373395
49. Results provide evidence to show that the N-CoR/HDAC3 co-repressor complex is involved in the aberrant transcription regulation in PML-RARalpha-expressing cells. PMID: 16730330
50. RB7 and butyrate induce dissociation of HDAC3 (but not HDAC1 or HDAC2) and its adaptor protein NCoR. PMID: 16849648
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亞細胞定位：

Nucleus.
蛋白家族：

N-CoR nuclear receptor corepressors family
數據庫鏈接：

HGNC: 7672

OMIM: 600849

KEGG: hsa:9611

STRING: 9606.ENSP00000268712

UniGene: Hs.462323