Recombinant Human Mitogen-activated protein kinase kinase kinase 3 (MAP3K3)

中文名稱：

人MAP3K3重組蛋白
貨號：

CSB-YP013425HU
規格：
來源：

Yeast
其他：

中文名稱：

人MAP3K3重組蛋白
貨號：

CSB-EP013425HU
規格：
來源：

E.coli
其他：

中文名稱：

人MAP3K3重組蛋白
貨號：

CSB-EP013425HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人MAP3K3重組蛋白
貨號：

CSB-BP013425HU
規格：
來源：

Baculovirus
其他：

中文名稱：

人MAP3K3重組蛋白
貨號：

CSB-MP013425HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

MAP3K3
Uniprot No.：

Q99759
別名：

M3K3_HUMAN; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; Mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-626
氨基酸序列

MDEQEALNSI MNDLVALQMN RRHRMPGYET MKNKDTGHSN RQSDVRIKFE HNGERRIIAF SRPVKYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL DKAIDILDRS SSMKSLRILL LSQDRNHNSS SPHSGVSRQV RIKASQSAGD INTIYQPPEP RSRHLSVSSQ NPGRSSPPPG YVPERQQHIA RQGSYTSINS EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF RKSRMSRAQS FPDNRQEYSD RETQLYDKGV KGGTYPRRYH VSVHHKDYSD GRRTFPRIRR HQGNLFTLVP SSRSLSTNGE NMGLAVQYLD PRGRLRSADS ENALSVQERN VPTKSPSAPI NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP ETSKEVSALE CEIQLLKNLQ HERIVQYYGC LRDRAEKTLT IFMEYMPGGS VKDQLKAYGA LTESVTRKYT RQILEGMSYL HSNMIVHRDI KGANILRDSA GNVKLGDFGA SKRLQTICMS GTGMRSVTGT PYWMSPEVIS GEGYGRKADV WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL RRIFVEARQR PSAEELLTHH FAQLMY
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
基因功能參考文獻：
1. MEKK3 KO reduced both EMT and cell migration, the size of 3D colonies and the percentage of CD44(+)/CD24(+)/EpCAM(+) CSC, promoter recruitment of YAP/TAZ and the expression of their target genes. PMID: 29599309
2. All these suggest that the MAP3K M1P site is a potential interacting partner of MAP3K SH3 domain, which may mediate the intermolecular recognition between hPTTG1 and MAP3K. PMID: 27787230
3. Polymorphisms in MAP3K3, MMP24 and IGF1R are associated with greater height and act additively on height in children of an admixed population. PMID: 26304632
4. MAP3K3 overexpression is an independent poor prognostic indicator in ovarian carcinoma. PMID: 26997451
5. MAP3K3 may potentially not only serve as diagnostic/prognostic markers for patients with lung cancer but also provide an indicator for future investigations into immunomodulatory therapies for lung cancer. PMID: 26088427
6. studies identify gain of MEKK3 signalling and KLF2/4 function as causal mechanisms for cerebral cavernous malformations pathogenesis that may be targeted to develop new CCM therapeutics PMID: 27027284
7. High MEKK3 expression is associated with renal clear cell carcinoma. PMID: 25824786
8. MEKK3 expression was significantly higher in patients with renal clear cell carcinoma than in controls. PMID: 25388155
9. This study identified an NBR1-MEKK3 complex as a key regulator of JNK signaling and adipose tissue inflammation in obesity. PMID: 25043814
10. Our finding that Verrucous venous malformation contains a MAP3K3 mutation supports our impression that this lesion is a venous anomaly. PMID: 25728774
11. MEKK3 expression was positively correlated with survivin. PMID: 25040987
12. Inhibitors of apoptosis proteins regulate myogenic differentiation by directly suppressing MEKK2/3-MEK5-ERK5 signaling. PMID: 24975362
13. Alterations in MEKK3 expression occur in early stages of development of esophageal squamous cell carcinoma and are sustained during disease progression. PMID: 24383423
14. We provide evidence for an intimate mutual control of the IKK complex by mitogen-activated protein kinase kinase kinase 3 (MEKK3) and transforming growth factor beta activated kinase 1 (TAK1). PMID: 24418622
15. Proliferation of human IVD cells is regulated by exogenous and autocrine growth factors mainly via the MEK/ERK and PI-3K/Akt pathways. PMID: 22105580
16. The regulation of NHEJ by EGFR was only blocked when ERK was affected by siRNA but not when AKT was knocked down. These data indicate that EGFR modulates DSB repair by regulating NHEJ via MAPK signalling. PMID: 20615764
17. EGFR and MAPK are actively involved in the pathobiology of serous cystic neoplasms of the pancreas. PMID: 20495538
18. PP2A plays an important role in the termination of LPA-mediated NF-kappaB activation through dephosphorylating and inactivating MEKK3 PMID: 20448038
19. we report the identification of two regulatory phosphorylation sites at Thr-516 and Ser-520 within the kinase activation loop that is essential for MEKK3-mediated IkappaB kinase beta (IKKbeta)/NF-kappaB activation PMID: 20068038
20. The rear end acidic cluster of the p62 PB1 domain is used to organize cytosolic aggregates or speckles-associated TRAF6-p62-MEKK3 complex for control of NF-kappaB activation. PMID: 19903815
21. results established that elevated expression of mitogen-activated protein kinase kinase kinase 3(MEKK3) appears to be a frequent occurrence in breast and ovarian cancers PMID: 14662759
22. MEKK3 signaling and apoptosis is potentiated by TRAF7 PMID: 15001576
23. MEKK3 has a role in NF-kappaB activation by the chemopreventive dithiolethione oltipraz PMID: 15047705
24. RIP-dependent recruitment of MEKK3 plays a specific role in TNF-alpha signaling. PMID: 15572679
25. We find that while hKSR-2 blocks MEKK3 activation, it has little to no effect on other members of the MAP3K family, including MEKK4, TAK1, and Ras-Raf, suggesting that its effects are selective. PMID: 16039990
26. TAK1 is recruited to the TNF-R1 complex via RIP and likely cooperates with MEKK3 to activate NF-kappaB in TNF-alpha signaling PMID: 16260783
27. Serine526 is the only phosphorylation site within the activation loop of MEKK3; phosphorylation occurs in response to exogenous stimuli like osmotic stress. PMID: 16407301
28. TLR8-mediated MEKK3-dependent IKKgamma phosphorylation might play an important role in the activation of IKK complex, leading to IkappaBalpha phosphorylation PMID: 16737960
29. structural details of MEKK3 PB1 and its binding properties with mitogen-activated protein kinase kinase 5, human PB1. PMID: 17985933
30. identifies a potentially important regulatory step in MEKK3 signaling via dephosphorylation of Thr(294), which reduces 14-3-3 binding correlating with MEKK3 pathway activation PMID: 18308725
31. Specific ablation of MEKK3 in T cells of conditional knockout mice does not significantly alter thymic T cell development, but leads to a peripheral T cell homeostasis defect. PMID: 19265138
32. These results strongly suggested that Hsp90 could work as the molecular chaperone of MEKK3. PMID: 19560753
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蛋白家族：

Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
數據庫鏈接：

HGNC: 6855

OMIM: 602539

KEGG: hsa:4215

STRING: 9606.ENSP00000354927

UniGene: Hs.29282