Recombinant Human DNA-binding protein inhibitor ID-3 (ID3)

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中文名稱：

Recombinant Human DNA-binding protein inhibitor ID-3 (ID3)
品名簡稱：

Recombinant Human ID3 protein
貨號：

CSB-EP010969HU
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

ID3
Uniprot No.：

Q02535
別名：

Class B basic helix-loop-helix protein 25;Helix-loop-helix protein HEIR-1;ID-like protein inhibitor HLH 1R21;Inhibitor of DNA binding 3;Inhibitor of differentiation 3
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

31.2 kDa
表達區(qū)域：

1-119aa
氨基酸序列

MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELTPELVISNDKRSFCH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
基因功能參考文獻：
1. This review will discuss the current understanding of ID3 in excess fat accumulation and the potential for Estrogenic Endocrine Disruptors to influence susceptibility to obesity or metabolic disorders via ID3 signaling PMID: 29507860
2. Taken together, we found that Id3+ and CTLA-4+ endometrial cells were significantly higher in women with repeated implantation failure and recurrent miscarriage, suggesting the negative roles of these angiogenesis and immune tolerance markers involving in regulating endometrium receptivity. PMID: 28224680
3. The interactions between ID3 and the DNA damage mediator protein MDC1 suggest that ID3 participates in the DNA damage signaling pathway to maintain genomic stability. PMID: 29026069
4. Immunohistochemical detection of inhibitor of DNA binding 3 mutational variants in mature aggressive B-cell lymphoma. PMID: 26992947
5. The results of the present study revealed that overexpression of Id3 may serve as a novel strategy for inhibiting cisplatinsensitive lung cancer. PMID: 27176047
6. Upon Id3 transfection, A549 cells displayed decreased migratory and invasive capabilities, however, co-transfection of miRId3 and Id3 into A549 cells reversed the Id3-induced inhibitions of migratory and invasive capabilities. PMID: 26384138
7. ID3 has a role in the acquisition of molecular stem cell-like signature in microvascular endothelial cells PMID: 25665868
8. our data provide evidence that ID3 may play a critical role in regulating vascular endothelial cell survival and development of microvascular lesions induced by persistent environmental pollutants such as PCB153. PMID: 25090023
9. ID3 plays a potential role in the underlying regulatory mechanisms of metabolic health in human obesity. [Review] PMID: 25239768
10. Epigenetic changes in ID3, in combination with experiences of maltreatment, may confer risk for depression in children. PMID: 24655651
11. Data indicate association of inhibitor of differentiation 3 (ID3) single nucleotide polymorphism rs11574 directly with coronary artery pathology. PMID: 24603695
12. Importantly, the distinct ID3 expression patterns in acute leukemias (AL) indicate a specific deregulation of ID3 in the various types of AL and may support subtyping of AL. PMID: 24292846
13. the combined immunohistochemical detection of VPREB3 and ID3 is applicable to the routine diagnostic in case of mature aggressive B-cell lymphomas. PMID: 24493312
14. ID3 mutations are recurrent events in double-hit B-cell lymphomas. PMID: 24222112
15. High ID3 expression is associated with medulloblastoma seeding and is a poor prognostic factor, especially in patients with Group 4 tumors PMID: 23768125
16. The findings suggested that cooperation between ID3 inactivation and immunoglobulin-MYC gene translocation is a hallmark of Burkitt lymphomagenesis. PMID: 23143595
17. Overexpression of Id3 markedly promoted the proliferation and invasive capacity of MCF-7 cells; however, these effects were significantly suppressed by the overexpression of FHL2. PMID: 22882857
18. Overexpression of Id3 triggered apoptosis in A549 lung adenocarcinoma cells, implicating Id3 in negative control of tumor growth. PMID: 22151756
19. EGFR-AKT-Smad signaling promotes formation of glioma stem-like cells and tumor angiogenesis by ID3-driven cytokine induction. PMID: 21975932
20. The ID3 protein is expressed in prostate cancer, and is elevated with the increase of Gleason score. PMID: 21837949
21. the data establish dysregulation of the Id/bHLH axis as an early and sustained feature of ductal pathogenesis PMID: 21498546
22. The level of Id-3 protein expression was associated with the malignant potential of gastric tumors. PMID: 20080245
23. ID3 plays a role as an apoptosis inducer in response to X-ray irradiation via the regulation of endogenous beta-catenin level. PMID: 21030215
24. In serum samples of lung cancer patients, galectin 1-mediated Id3 induces expression of interleukin (IL)-10 in functionally altered signal pathways of monocytes and monocyte-derived dendritic cells. PMID: 21191065
25. Results provide novel evidence that Id3 is an atheroprotective factor and link a common SNP in the human ID3 gene to loss of Id3 function and increased carotid intima-media thickness. PMID: 20185798
26. nuclear Id protein acts by sequestering pools of transiently diffusing bHLH protein to prevent reassociation with chromatin domains PMID: 12952978
27. Fluvastatin did not regulate p21 and p27, but down-regulated Id3 and p53 slightly. PMID: 15370294
28. Overexpression of Id3 enhances expression of ICAM-1 and E-selectin, and induces angiogenic processes such as transmigration, matrix metalloproteinase-2 and -9 expression, and tube formation in cultured vascular endothelial cells. PMID: 15494533
29. ultraviolet radiation-induced apoptosis of immortalized keratinocytes is at least in part due to Id3 upregulation in these cells PMID: 16449966
30. estrogen-induced tube formation in vascular endothelial cells requires the presence of Id3, a member of the helix-loop-helix family of transcriptional factors and estrogen increases Id3 phosphorylation via a redox-dependent process PMID: 18281048
31. RhoA/Rho-associated kinase signaling plays positive and negative roles in myogenic differentiation, mediated by MRTF-A/Smad-dependent transcription of the Id3 gene in a differentiation stage-specific manner PMID: 18477564
32. There is a mechanism whereby reactive oxygen species upregulation of Id3 relieves repression of bax via E-box-binding factors. PMID: 19054058
33. RNA interference demonstrated that Id3 regulates differentiation and cell cycle (increased Neuro-D6 and p21 mRNA) in neuroblastoma cells PMID: 19477940
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亞細胞定位：

Nucleus.
組織特異性：

Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
數(shù)據(jù)庫鏈接：

HGNC: 5362

OMIM: 600277

KEGG: hsa:3399

STRING: 9606.ENSP00000363689

UniGene: Hs.76884