Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 6 (EBNA6), partial

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中文名稱：

Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 6 (EBNA6), partial
品名簡稱：

Recombinant Epstein-Barr virus EBNA6 protein, partial
貨號：

CSB-EP365875EFA
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

EBNA6
Uniprot No.：

P03204
別名：

Epstein-Barr nuclear antigen 3C;Epstein-Barr nuclear antigen 4B
種屬：

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
蛋白長度：

Partial
來源：

E.coli
分子量：

37.3 kDa
表達區域：

146-410aa
氨基酸序列

MAARQRLQDIRRGPLVAEGGVGWRHWLLTSPSQSWPMGYRTATLRTLTPVPNRVGADSIMLTATFGCQNAARTLNTFSATVWTPPHAGPREQERYAREAEVRFLRGKWQRRYRRIYDLIELCGSLHHIWQNLLQTEENLLDFVRFMGVMSSCNNPAVNYWFHKTIGNFKPYYPWNAPPNENPYHARRGIKEHVIQNAFRKAQIQGLSMLATGGEPRGDATSETSSDEDTGRQGSDVELESSDDELPYIDPNMEPVQQRPVMFVSR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays an essential role for the activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA6 probably contributes to a negative autoregulatory control loop. Alternatively, EBNA6 also regulates the transcription of the EBV oncogene LMP1 in a cell cycle-dependent manner. Modulates the activity of several host proteins involved in cell cycle regulation including host cyclin A, MYC, RB, p21 and p27 mainly through binding to the host SCF(SKP2) complex. Inhibits the promoter of host H2AX and targets H2AX to proteasomal degradation in order to promote latency and cell proliferation.
基因功能參考文獻：
1. Our results provide novel insights into the function of EBNA3C on cell progression by regulating the cyclin D2 protein and raise the possibility of the development of new anticancer therapies against EBV-associated cancers. PMID: 29997218
2. EBNA3B restrains the oncogenic capacity of EBV, so it acts as a tumour suppressor.[review] PMID: 26428372
3. EBNA3C is necessary to overcome an EBV-driven increase in p16(INK4a) expression and concomitant block to proliferation 2-4 weeks post-infection. PMID: 23436997
4. The p73-mediated apoptotic pathway is inhibited in the presence of EBNA3C. PMID: 24314664
5. During latency, out of a subset of viral genes expressed, EBNA-3C is one of the essential antigens required for in vitro primary B-cell transformation. PMID: 23464371
6. This study provided formal proof that when EBV represses BIM transcription in B cells, expression of both EBNA3A and EBNA3C is necessary for the assembly of PRC2 on the BIM promoter and the deposition of the epigenetic repressive mark H3K27me3. PMID: 22584624
7. EBNA3BKO virus caused highly proliferative, monomorphic B cell lymphomas in humanized mice. EBV+ human lymphoma samples had EBNA3B mutations. EBNA3B is a virus-encoded tumor suppressor. Loss promotes immune evasion & lymphomagenesis. PMID: 22406538
8. we have unveiled key cellular pathways repressed by EBNA 3C that are likely to contribute to the ability of EBV-immortalized cells to modulate immune responses, adhesion, and B-lymphocyte migration to facilitate persistence in the host. PMID: 22357270
9. EBNA3C gene variations are geographically restricted rather than tumor-specific polymorphisms. PMID: 22302288
10. we report that EBNA3C directly interacts with Gemin3 through its C-terminal domains PMID: 22174681
11. Epstein-Barr virus nuclear antigen 3C facilitates G1-S transition by stabilizing and enhancing the function of cyclin D1. PMID: 21347341
12. EBNA3C negatively regulate p53-mediated functions by interacting with ING4 and ING5. PMID: 21177815
13. EBV nuclear antigen 3C (EBNA3C) is an essential transcription factor for EBV transformed lymphoblast cell line growth, regulating 550 genes, 106 of which are recognized in protein networks. PMID: 21173222
14. EBNA3C interacts with Gadd34, activating the upstream component of the unfolded protein response UPR (eIF2alpha phosphorylation) while preventing downstream UPR events (XBP1 activation and ATF6 cleavage). PMID: 20040105
15. EBNA3C forms a stable complex with Rb in cells when the proteasome machinery is inhibited and interacts with Rb in vitro, mapping to a conserved domain at the terminus of EBNA3C PMID: 16352731
16. findings show that both EBNA3C and Rhesus EBNA3C bind to a conserved region of mammalian cyclins, regulate pRb stability, and modulate SCF(Skp2)-dependent ubiquitination PMID: 16364389
17. current knowledge of functions of EBNA 3C, the roles played by the different domains in these functions, and summary of recent work that provides the first structural and functional analysis of the putative bZIP domain of EBNA 3C [review] PMID: 16611138
18. The effects of the EBV nuclear antigen 3C on the activities of Nm23-H1 via GATA-1 and Sp1 are reported. PMID: 16631833
19. results indicate that EBNA-3C directly activates the expression of LMP-1 and LMP-2B but is unlikely to significantly regulate EBNA expression via Cp under normal growth conditions.[EBNA-3C] PMID: 16956945
20. identification of a number of potential EBNA-3B- and EBNA-3C-regulated genes in the context of EBV-infected lymphoblastoid cell lines PMID: 17005691
21. Epstein-Barr virus nuclear protein EBNA3C is required for cell cycle progression and growth maintenance of lymphoblastoid cells. PMID: 17159137
22. EBNA3C can directly regulate the G2/M component of the host cell cycle machinery through ATM/ATR and Chk2, allowing for the release of the checkpoint block PMID: 17409144
23. In the absence of Bim, EBNA3A and EBNA3C appear to provide no survival advantage in Burkitt's lymphoma cells. PMID: 17653091
24. EBNA-6/EBNA-3C binds to a MRPS18-2 protein, and targets it to the nucleus; EBNA-6 may play a major role in the entry of EBV infected B cells into the S phase by binding to and raising the level of nuclear MRPS18-2, protein. PMID: 18391203
25. EBNA3C can modulate Necdin-associated functions in association with the metastasis suppressor protein Nm23-H1. PMID: 19116252
26. EBNA3C regulation of transcription through RBP-Jkappa is critical to maintaining lymphoblastoid cell growth PMID: 19237563
27. Epstein-Barr virus nuclear antigen 3C, one of the EBV latent antigens essential for the B-cell immortalization in vitro, interacts directly with p53 PMID: 19394062
28. The authors confirmed that EBNA3C upregulates TCL1 and discovered that EBNA3C upregulates TCL1 through RBP-Jkappa, indicating a central role for EBNA3C interaction with RBP-Jkappa in mediating cell gene transcription. PMID: 19776126
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亞細胞定位：

Host nucleus. Host nucleus matrix. Note=Associated with the nuclear matrix.
蛋白家族：

Herpesviridae EBNA-6 family
數據庫鏈接：

KEGG: vg:3783763