Recombinant Enterobacteria phage T4 Recombination protein uvsY (uvsY)

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中文名稱：

Recombinant Enterobacteria phage T4 Recombination protein uvsY(uvsY)
品名簡稱：

Recombinant Enterobacteria phage T4 uvsY protein
貨號：

CSB-EP366179EDZ
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP366179EDZ could indicate that this peptide derived from E.coli-expressed Enterobacteria phage T4 (Bacteriophage T4) uvsY.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP366179EDZ could indicate that this peptide derived from E.coli-expressed Enterobacteria phage T4 (Bacteriophage T4) uvsY.
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產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

uvsY
Uniprot No.：

P04537
別名：

uvsY; Recombination protein uvsY
種屬：

Enterobacteria phage T4 (Bacteriophage T4)
蛋白長度：

Full Length
來源：

E.coli
分子量：

31.8kDa
表達區域：

1-137aa
氨基酸序列

MRLEDLQEELKKDVFIDSTKLQYEAANNVMLYSKWLNKHSSIKKEMLRIEAQKKVALKARLDYYSGRGDGDEFSMDRYEKSEMKTVLSADKDVLKVDTSLQYWGILLDFCSGALDAIKSRGFAIKHIQDMRAFEAGK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Intact Enterobacteria phage T4 Recombination protein uvsY (uvsY) cDNA (1-137aa) with an N-terminal 6xHis--SUMO-tag was expressed in the E.coli. The forming protein is the Recombinant full-length Enterobacteria phage T4 uvsY protein. The purity of this protein is greater than 90% as determined by SDS-PAGE. Under reducing conditions, the SDS-PAGE gel showed a molecular weight band of about 33 kDa. And this uvsY protein also underwent the validation by the LC-MS/MS analysis. This uvsY protein is in-stock so that there is no waiting period for product preparation. It may find uses for specific antibody synthesis or in the studies of homologous recombination (HR).

UVSY is the phage T4 recombination mediator protein (RMP) that is crucial for efficient HR in bacteriophage T4 and is the functional analog of the eukaryotic Rad52 protein. HR has important roles in repairing double-stranded DNA (dsDNA) break repair, restarting the stalled replication forks, and recombination-dependent replication. UVSY is specifically recruited to ssDNA–Gp32 complexes and promotes the dissociation of Gp32, thus stimulating the DNA-dependent ATPase activity of recombinase UVSX and facilitating the strand exchange.
Datasheet & COA：

Please contact us to get it.

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功能：

Plays a role in viral DNA synthesis by promoting enzymatic activities of UvsX recombinase, by promoting UvsX-ssDNA filament assembly, and by helping UvsX to displace bound gp32 from ssDNA.
基因功能參考文獻：
1. UvsY globally stabilizes UvsX-ssDNA complexes, consistent with an increase in the apparent equilibrium binding affinity, K(ss)omega, of the UvsX-ssDNA interactions. PMID: 16634631
2. results support a mechanism in which the formation of competent presynaptic filaments requires a hand-off of ssDNA from UvsY to UvsX, with the efficiency of the hand-off controlled by the relative ssDNA-binding affinities of the two proteins. PMID: 19244311
數據庫鏈接：

KEGG: vg:1258547