Recombinant Arabidopsis thaliana FAD-linked sulfhydryl oxidase ERV1 (ERV1)
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中文名稱:擬南芥ERV1重組蛋白
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貨號(hào):CSB-EP810165DOA-B
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說明書:
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規(guī)格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:ERV1
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Uniprot No.:
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別名:ERV1; At1g49880; F10F5.3FAD-linked sulfhydryl oxidase ERV1; AtErv1; EC 1.8.3.2; Mitochondrial sulfhydryl oxidase ERV1
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種屬:Arabidopsis thaliana (Mouse-ear cress)
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蛋白長度:full length protein
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表達(dá)區(qū)域:1-191
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氨基酸序列MGEKPWQPLL QSFEKLSNCV QTHLSNFIGI KNTPPSSQST IQNPIISLDS SPPIATNSSS LQKLPLKDKS TGPVTKEDLG RATWTFLHTL AAQYPEKPTR QQKKDVKELM TILSRMYPCR ECADHFKEIL RSNPAQAGSQ EEFSQWLCHV HNTVNRSLGK LVFPCERVDA RWGKLECEQK SCDLHGTSMD F
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蛋白標(biāo)簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點(diǎn)詳情
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功能:FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Oxidizes thioredoxin in vitro. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space. Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state.
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基因功能參考文獻(xiàn):
- Plant Erv1, unlike its homologs in fungi and animals, can promote protein import and oxidative protein folding in the IMS independently of the oxidoreductase Mia40. PMID: 29117860
- Results describe the structure of Erv1, a sulfhydryl oxidase that contains a Cys-X4-Cys shuttle disulfide and oxidizes thioredoxin in vitro PMID: 16893552
- Mutants of AtErv1 lacking each of the three individual cysteine pairs were studied to determine the involvement of the respective disulfide groups in these reactions PMID: 19017646
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亞細(xì)胞定位:Mitochondrion.
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數(shù)據(jù)庫鏈接:
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