CD99 Recombinant Monoclonal Antibody

說明書

中文名稱：

CD99重組抗體
貨號：

CSB-RA004973A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in Jurkat whole cell lysate
All lanes CD99 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 KDa
Observed band size: 28 KDa

IHC image of CSB-RA004973A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Overlay histogram showing Jurkat cells stained with CSB-RA004973A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CD99重組單克隆抗體（CSB-RA004973A0HU）是針對人類CD99抗原設計的高特異性檢測工具，適用于多種實驗場景。CD99是一種在細胞膜表面廣泛表達的跨膜糖蛋白，參與調控細胞黏附、遷移及免疫細胞活化等生物學過程，尤其在T淋巴細胞發育和某些血液系統疾病中具有重要研究價值。本抗體經ELISA、蛋白質印跡（WB）、免疫組化（IHC）和流式細胞術（FC）等多平臺嚴格驗證，在WB實驗中可識別天然和變性狀態下的靶蛋白，推薦使用稀釋比例為1:500-1:5000；IHC實驗在石蠟包埋組織切片中可清晰顯示膜定位信號，推薦稀釋比例1:50-1:500；流式檢測則能有效識別細胞表面表達的CD99分子。該重組抗體采用單克隆技術生產，具有優異的批次間一致性和低交叉反應性，適用于腫瘤生物學研究、免疫細胞分型分析及病理樣本中CD99表達模式的檢測，可為探究細胞遷移機制、免疫調控網絡及血液系統疾病相關研究提供可靠工具。
Uniprot No.：

P14209
基因名：

CD99
別名：

CD99; MIC2; MIC2X; MIC2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD antigen CD99
反應種屬：

Human
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9A5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.
基因功能參考文獻：
1. Besides confirming the importance of CD99, findings indicate that polymorphic variations in this gene may affect either development or progression of EWS. PMID: 27792997
2. CD99 is a marker of acute myeloid leukemia and myelodysplastic syndrome stem cells PMID: 28123069
3. CD99 expression is increased in patients with active Inflammatory bowel disease, and positively correlated with disease activity. PMID: 27866429
4. CD99 counteracts EWS-FLI1 in controlling NF-kappaB signaling through the miR-34a, which is increased and secreted into exosomes released by CD99-silenced Ewing sarcoma cells. PMID: 26616853
5. these observations suggest that CD99 is involved in the regulation of CD1a transcription and expression by increasing ATF-2. PMID: 27094031
6. CD99 was also paranuclear positive in 4 of 11 (36%) small cell lung carcinomas PMID: 26782152
7. High expression of EMMPRIN plays a crucial role in breast cancer cell proliferation, matrigel invasion and tumor formation. PMID: 27002769
8. Data show that CD99 antigen expression increased during in vitro differentiation of germinal center B cells and was highest in plasma cells (PCs). PMID: 26522646
9. Our results suggest that the CD34/CD25/CD123/CD99(+) LAIP is strictly associated with FLT3-ITD-positive cells. PMID: 25957287
10. The results highlight an important role of CD99 in the differentiation and activity of human osteoblasts in physiological and pathological conditions. PMID: 26000312
11. CD99 can suppress CD98-mediated assembly of pro-tumorigenic signaling complexes through its ability to dephosphorylate focal adhesion kinase. PMID: 26172215
12. CD99 expression in astrocytomas of different malignant grades might contribute to the infiltrative ability and support the importance of CD99 as a potential target to reduce infiltrative astrocytoma capacity in migration and invasion. PMID: 24797829
13. The dAbd C7 induced rapid and massive EWS cell death through Mdm2 degradation and p53 reactivation. Mdm2 overexpression as well as silencing of p53 in p53wt EWS cells decreased CD99-induced EWS cell death PMID: 25501132
14. CD99 expression in nasal lobular capillary haemangioma. PMID: 24702676
15. dermatofibromastrongly expresses CD99 in a diffuse pattern that may serve as evidence in distinction from dermatofibrosarcoma PMID: 24247571
16. CD99 induces ERK activation, increasing its membrane-bound/cytoplasmic form rather than affecting its nuclear localization. PMID: 24677094
17. The new antibody scFvC7 recognizes the CD99 extracellular domain. PMID: 22335486
18. Report purification of human antibody scFv anti-CD99 for blocking monocyte transendothelial migration. PMID: 24798881
19. Solid pseudopapillary neoplasm of the pancreas exhibits a very unique immunostaining pattern for CD99 that is present in all cases. PMID: 24094957
20. we have shown that forced expression of wild-type CD99 in osteosarcoma cells induces downregulation of genes such as ROCK2 PMID: 23644663
21. CXCL16, inversely correlated with CD99 expression in Hodgkin Reed-Sternberg (H/RS) cells. PMID: 23743627
22. It is assumed that the expression of CD99 plays an important role in the pathogenesis of psoriasis, in the processes of emigration of leukocytes, and in their tropism toward to the epidermis PMID: 24341229
23. we show that miR-30a-5p provides an essential link between EWS-FLI1 and CD99, two major biomarkers in Ewing sarcoma PMID: 22986530
24. CD99 is highly expressed in anaplastic large-cell lymphoma, and showed high rate of co-expression with ALK. PMID: 22781601
25. PVR resides in the recently identified lateral border recycling compartment, similar to PECAM and CD99. PMID: 23333754
26. We report an unusual pattern of paranuclear dot-like expression of CD99 in 14 cases of Merkel cell carcinoma, two of which did not express CK20. PMID: 23145531
27. CD99-induced HSP70 expression in B and T lymphocytes. PMID: 23152061
28. Data suggest that CD99 expression in lymph node/germinal center biopsies at time of diagnosis is not prognostic marker for survival in patients with diffuse large B-cell lymphoma treated with rituximab-CHOP immunochemotherapy. PMID: 22864685
29. the membrane protein CD99 was identified as a novel stromal factor with clinical relevance. The results support the concept that stromal properties have an important impact on tumour progression. PMID: 22392539
30. upregulation of CD99 in H/RS cells induces terminal B-cell differentiation, which may provide a novel therapeutic strategies for cHL PMID: 22020966
31. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) and CD99 are critical in lymphatic transmigration of human dendritic cells. PMID: 22189791
32. NF-kappaB2 exhibits the major inhibitory role in the transcription at the CD99 promoter PMID: 22083306
33. High CD99 is associated with central primitive neuroectodermal tumors and Ewing's sarcoma PMID: 21267687
34. Data show that CD99 combined with E-cadherin/beta-catenin and CD10 can be used as a relatively specific expression profile of SPTs. PMID: 21775056
35. Our study has identified for the first time that pancreatic solid-pseudopapillary neoplasm exhibits a unique dot-like staining pattern for CD99. PMID: 21566515
36. CD99 positivity was significantly associated with advanced stage (p < 0.01), higher risk group according to the International Prognostic Index risk score (p < 0.01) and non-germinal center B cell-like type (p = 0.01). PMID: 21196719
37. Protein expression and gene promoter hypermethylation of CD99 in transitional cell carcinoma of urinary bladder PMID: 20217126
38. CD99 showed no preferential staining of Atypical fibroxanthoma, spindle cell malignant melanoma or sarcomatoid carcinoma/spindle cell squamous cell carcinoma PMID: 20184665
39. CD99 may play a physiologic role in the clonal deletion processes necessary for B-lymphoid selection PMID: 20453109
40. Analysis of a panel of human EWS cells revealed an inverse correlation between CD99 and H-neurofilament expression, as well as an inverse correlation between neural differentiation and oncogenic transformation. PMID: 20197622
41. Results suggest that type I is the major isoform of CD99 expressed in non-neoplastic gastric mucosa and gastric adenocarcinomas. PMID: 12172043
42. Functional involvement of src and focal adhesion kinase in a CD99 splice variant-induced motility of human breast cancer cells PMID: 12216109
43. engagement triggers the exocytic transport of ganglioside GM1 and the reorganization of actin cytoskeleton PMID: 12681511
44. inhibition by MHC class I engagement or apoptosis and upregulation of T cell receptor and MHC molecules in human thymocytes and T cell line induced by CD99 PMID: 12832073
45. CD99 epitopes plays a distinct role in T cell biology, especially in T cell apoptosis. PMID: 14623115
46. CD99 type II acts as a negative regulator in the neural differentiation of precursor cells that might occur during nerve system development PMID: 14646598
47. Solitary sclerotic fibroma is a fibrotic lesion with cells positive for CD34 and O13. O13 expression in SF has not been previously described. PMID: 14744088
48. our findings indicate that CD99 functions occur through reorganization of cytoskeleton and identify actin and zyxin as the early signaling events driven by CD99 engagement. PMID: 15184883
49. solution structure of cytoplasmic domain of human CD99 type I shows that it has a hairpin shape anchored by two flexible loops PMID: 15359120
50. cyclophilin A binds CD99 and may be either a signaling mediator or a signaling regulator for CD99 PMID: 15388255
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

CD99 family
數據庫鏈接：

HGNC: 7082

OMIM: 313470

KEGG: hsa:4267

STRING: 9606.ENSP00000370588

UniGene: Hs.653349

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